Lower-serum and efficient culture medium of mycoplasma ovipneumoniae and preparation method of lower-serum and efficient culture medium

Abstract

The invention discloses a lower-serum and efficient culture medium of mycoplasma ovipneumoniae. The lower-serum and efficient culture medium is prepared from the following components: MEM, sodium pyruvate, glucose, Hank's liquid, fresh yeast leaching liquid, L-glutamine, L-cysteine, lactalbumin hydrolysate, calf thymus DNA (Deoxyribonucleic Acid), insulin, transferrin, penicillin, horse serum, phenol red and de-ionized water; the invention provides a preparation method of the lower-serum and efficient culture medium. The lower-serum and efficient culture medium of the mycoplasma ovipneumoniae, disclosed by the invention, has the beneficial effects that the content of the serum is only 5 percent and is 1 / 4 of the content of the serum in a culture medium in the prior art; the titer of mycoplasma ovipneumoniae semi-finished-product bacterium liquid prepared by the method reaches 10<10>CCU / ml and is obviously higher than that of the culture medium in the prior art. According to the lower-serum and efficient culture medium of the mycoplasma ovipneumoniae, disclosed by the invention, the sensitive stress response to a goat body, caused by heterologous serum, is alleviated, and the biosafety is improved; the titer of bacterium liquid of living bacteria is also improved and the production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of veterinary preparations, in particular to a low-serum high-efficiency medium for Mycoplasma pneumoniae ovis and a preparation method thereof. Background technique [0002] Mycoplasma ovipneumoniae (Mo) can cause interstitial pneumonia in sheep and goats. The clinical features are cough, wheezing, runny nose, emaciation, anemia, and growth retardation. In 1963, Mackay et al first isolated Mycoplasma ovis pneumoniae from the diseased lung of Scottish sheep. Mycoplasma ovis pneumoniae mainly infects sheep and goats. At present, there are reports of the incidence of sheep and goats caused by Mycoplasma ovis pneumoniae in many countries around the world, causing huge economic losses to the world sheep industry. In 1978, Chinese scholars isolated Mycoplasma ovis pneumoniae for the first time in Sichuan Province from offspring lambs of Leicester sheep introduced from New Zealand. Subsequently, many provinces a...

AI Technical Summary

Problems solved by technology

There are problems such as long culture time, low titer of live bacteria and poor reproductive ability in culturing Mycoplasma pneumoniae in the culture medium of the prior art

In addition, the serum content in the culture medium of the prior art is generally 20%. Vaccines prepared with high serum content will undoubtedly increase the allergic stress response of the heterologous serum to the sheep body, which will eventually affect the immune effect of the vaccine and increase the cost of vaccine production.

If the serum content in the culture medium of the prior art is simply reduced, the antigen titer of Mycoplasma ovis will be lowered by about 10 to 100 times, and the antigen dose required for immunization will not be reached, and the concentration multiple needs to be increased, which actually increases the production cost.

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