Preparation method of pathogenic vibrio harveyi DNA (Deoxyribonucleic Acid) template in prawn culture

Abstract

The invention discloses a preparation method of a pathogenic vibrio harveyi DNA (Deoxyribonucleic Acid) template in prawn culture. The preparation method comprises the following steps: carrying out shaking-bed culture on three test tubes with a liquid culture medium at 28 DEG C for one night; taking out the three test tubes the next day; taking one test tube as a control group and taking two test tubes as experiment groups; opening a clean worktable; firstly, carrying out ultraviolet disinfection for 20min and then putting the test tubes into the clean worktable; selecting one test tube with bacteria, which has a relatively great concentration; sucking 1ml of a bacterium culture solution into a prepared PCR (Polymerase Chain Reaction) tube by utilizing a pipette; adding an equal amount of clean water into the other PCR tube, so as to conveniently symmetrize in a centrifuging process. The preparation method disclosed by the invention is simple and has a simple preparation effect; the template can be used for effectively detecting vibrio infection in a culture water area or tissues in cultured prawns; water quality regulation and control can be carried out in advance, prawn vibrio diseases are prevented early and large-scale outbreak of diseases is prevented and controlled, so that healthy culture of the prawns is effectively improved.

Description

Technical field [0001] The present invention relates to the technical field of Vibrio harveyi, in particular to a method for preparing DNA template of pathogenic Vibrio harveyi in shrimp breeding. Background technique [0002] At present, the main disease of prawn culture (Penaeus chinensis, Penaeus japonicus, Litopenaeus vannamei) is white spot syndrome (WSSV). In 124 batches of prawn samples, the positive rate of WSSV is above 80%; the second is Arc Vibrio harveyi is the most important pathogenic factor. In 209 batches of prawn samples, the positive rate of Vibrio harveyi is more than 70%. [0003] Vibrio harveyi not only can directly cause the disease in the cultured prawns, the appendages of the prawns will turn red, the swimming feet will turn red at the earliest, the walking feet and tail limbs will also be bright red, the gill area of ​​the cephalothorax is yellow, anorexia, and motivation The phenomenon of weakening and individual weight loss will also quickly cause the in...

AI Technical Summary

Problems solved by technology

[0003] Vibrio harveyi can not only directly cause disease in cultured prawns, but the appendages of prawns turn red, the swimming feet turn red first, the legs and tail limbs are also bright red, the gill area of ​​the carapace is yellow, anorexia, motility Weakening, individual emaciation, and rapid infection of prawns in the same aquaculture water, resulting in a large-scale outbreak of red leg disease, resulting in rapid and massive death of prawns, which is very harmful