Preparation method for microencapsulated biopesticide

A biopesticide and microencapsulation technology, which is applied in botany equipment and methods, preservation of human or animal bodies, biocides, etc., can solve the problems of prolonging the time of drug effect, short period of drug effect, and specific prevention and control objects, etc. problems, to achieve the effect of avoiding pesticide residues, reducing crop diseases and insect pests, and broad-based control

Inactive Publication Date: 2017-05-17
汪逸凡
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem mainly solved by the present invention: for the existing biopesticides, which are specific for prevention and control, do not have broad efficacy, and at the same time have a short period of drug effect, and the existing biopesticides have poor storage stability and short shelf life. The defect is to provide a preparation method of microencapsulated biopesticides. The present invention first freezes and inactivates Tenebrio molitor to make powder, collects the filtrate and filter residue after ultrasonic extraction, and distills the filtrate under reduced pressure to obtain Tenebrio molitor containing pheromones. Extract the oil, sterilize the filter residue and inoculate Bacillus thuringiensis for fermentation, then filter the primary treatment fermentation liquid, purify and separate it by isoelectric point precipitation to obtain parasporal crystals, and then use it and Tenebrio molitor extract oil as the core material, Chitosan and soybean protein isolate are used as wall materials, and after shear emulsification treatment and spray drying, microencapsulated biopesticides can be obtained. The microencapsulated biopesticides prepared by the present invention are combined with paraspore crystals with broad-effective control effects and containing Insect pheromones and Tenebrio molitor oil act synergistically, and are wrapped with broad-spectrum antibacterial chitosan, which can effectively prolong the drug effect time, and have good storage stability, which is convenient for transportation and long-term storage, and can effectively prevent and control plant diseases and insect pests

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019]First weigh 800g Tenebrio molitor, place it in a clean breeding box for starvation treatment for 20 hours, then rinse it twice with deionized water, and soak it in 500mL of 1.5% sodium chloride solution for 1 hour to obtain pretreated Tenebrio molitor. Transfer the pretreated Tenebrio molitor into a freeze-drying box, and freeze-dry it at -55°C for 10 hours to obtain freeze-inactivated dry Tenebrio molitor; then weigh 80 g of the above-mentioned freeze-inactivated dried Tenebrio molitor, put it into a tissue masher and pulverize it, Pass through a 60-mesh sieve, mix the sieved Tenebrio molitor powder with 800mL methanol, put it into a Soxhlet extractor, and use a 350W ultrasonic wave to assist extraction for 40 minutes, then filter, collect the filter residue, and obtain Tenebrio molitor residue, and then decompress the filtrate Distill to 20% of the volume of the stock solution to obtain Tenebrio molitor extract oil, which is set aside; then mix the above Tenebrio molito...

example 2

[0022] First weigh 900g Tenebrio molitor, place it in a clean breeding box for starvation treatment for 22 hours, then rinse twice with deionized water, soak in 650mL mass fraction of 1.5% sodium chloride solution for 1 hour, and obtain pretreated Tenebrio molitor, Transfer the pretreated Tenebrio molitor into a freeze-drying box, and freeze-dry it at -50°C for 11 hours to obtain freeze-inactivated dry Tenebrio molitor; then weigh 90 g of the above-mentioned freeze-inactivated dried Tenebrio molitor, put it into a tissue masher and pulverize it, Pass through a 60-mesh sieve, mix the sieved Tenebrio molitor powder with 900mL methanol, put it into a Soxhlet extractor, and extract it with 350W ultrasonic ultrasound for 45 minutes, then filter, collect the filter residue, and obtain Tenebrio molitor residue, and decompress the filtrate Distill to 25% of the volume of the stock solution to obtain the Tenebrio molitor extract oil, and set aside; then mix the above Tenebrio molitor re...

example 3

[0025] First weigh 1000g Tenebrio molitor, put it in a clean feeding box and starve it for 24 hours, then rinse it with deionized water for 3 times, then soak it in 800mL of 1.5% sodium chloride solution for 2 hours to get pretreated Tenebrio molitor, Transfer the pretreated Tenebrio molitor into a freeze-drying box, and freeze-dry it at -45°C for 12 hours to obtain freeze-inactivated dry Tenebrio molitor; then weigh 100 g of the above-mentioned freeze-inactivated dried Tenebrio molitor, put it into a tissue masher and pulverize it, Pass through a 60-mesh sieve, mix the sieved Tenebrio molitor powder with 1000mL methanol, put it into a Soxhlet extractor, and use 350W ultrasonic ultrasound to assist extraction for 50 minutes, then filter, collect the filter residue, and obtain Tenebrio molitor residue, and then decompress the filtrate Distill to 30% of the volume of the stock solution to obtain Tenebrio molitor extract oil, which is set aside; then mix the above Tenebrio molitor...

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PUM

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Abstract

The invention discloses a preparation method for microencapsulated biopesticide, and belongs to the technical field of pesticide preparation. The preparation method comprises the following steps: first, freezing and inactivating yellow mealworms into powder; performing ultrasonic extraction, and respectively collecting a filtrate and a filter residue; distilling the filtrate under reduced pressure to obtain pheromone-containing yellow mealworm extracting oil; sterilizing the filter residue, performing inoculation with bacillus thuringiensis for fermentation, and then, purifying and separating primarily treated fermenting solution which is obtained by filtering through an isoelectric precipitation method to obtain parasporal crystals; taking the parasporal crystals and the yellow mealworm extracting oil as a core material, and taking chitosan and soybean protein isolate as wall materials, performing shearing and emulsifying treatment, and performing spray-drying to obtain the microencapsulated biopesticide. Through a synergistic effect of the parasporal crystals with a broad-spectrum preventing and controlling effect and the yellow mealworm oil containing insect pheromones, the microencapsulated biopesticide prepared with the preparation method is coated with chitosan having the broad-spectrum antibacterial property, so that the pesticidal effect action time can be effectively prolonged; the storage stability is high; the microencapsulated biopesticide is convenient to transport and store for a long time; diseases and insect pests of plants can be effectively prevented and controlled.

Description

technical field [0001] The invention discloses a preparation method of microencapsulated biological pesticide, which belongs to the technical field of pesticide preparation. Background technique [0002] The application of chemical pesticides, especially organic synthetic chemical pesticides is a major symbol of the development of human civilization, which greatly protects and develops the productivity of human society. In comparison, artificially synthesized chemical pesticides have the advantages of strong toxicity, quick effect, and the ability to quickly inhibit the occurrence and spread of pests and diseases in a large area. Exposed, there are many problems such as pesticide residues, pesticide resistance, environmental pollution, non-target biological damage and chronic toxicity. Residual pesticides enter the human body through the food chain and gradually accumulate, which can easily induce cancer and other difficult diseases, and even cause casualties. [0003] At ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N25/28A01P7/04
CPCA01N25/28A01N63/10A01N2300/00
Inventor 汪逸凡宋豪薛蕾
Owner 汪逸凡
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