Preparation method for microencapsulated biopesticide
A biopesticide and microencapsulation technology, which is applied in botany equipment and methods, preservation of human or animal bodies, biocides, etc., can solve the problems of prolonging the time of drug effect, short period of drug effect, and specific prevention and control objects, etc. problems, to achieve the effect of avoiding pesticide residues, reducing crop diseases and insect pests, and broad-based control
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example 1
[0019]First weigh 800g Tenebrio molitor, place it in a clean breeding box for starvation treatment for 20 hours, then rinse it twice with deionized water, and soak it in 500mL of 1.5% sodium chloride solution for 1 hour to obtain pretreated Tenebrio molitor. Transfer the pretreated Tenebrio molitor into a freeze-drying box, and freeze-dry it at -55°C for 10 hours to obtain freeze-inactivated dry Tenebrio molitor; then weigh 80 g of the above-mentioned freeze-inactivated dried Tenebrio molitor, put it into a tissue masher and pulverize it, Pass through a 60-mesh sieve, mix the sieved Tenebrio molitor powder with 800mL methanol, put it into a Soxhlet extractor, and use a 350W ultrasonic wave to assist extraction for 40 minutes, then filter, collect the filter residue, and obtain Tenebrio molitor residue, and then decompress the filtrate Distill to 20% of the volume of the stock solution to obtain Tenebrio molitor extract oil, which is set aside; then mix the above Tenebrio molito...
example 2
[0022] First weigh 900g Tenebrio molitor, place it in a clean breeding box for starvation treatment for 22 hours, then rinse twice with deionized water, soak in 650mL mass fraction of 1.5% sodium chloride solution for 1 hour, and obtain pretreated Tenebrio molitor, Transfer the pretreated Tenebrio molitor into a freeze-drying box, and freeze-dry it at -50°C for 11 hours to obtain freeze-inactivated dry Tenebrio molitor; then weigh 90 g of the above-mentioned freeze-inactivated dried Tenebrio molitor, put it into a tissue masher and pulverize it, Pass through a 60-mesh sieve, mix the sieved Tenebrio molitor powder with 900mL methanol, put it into a Soxhlet extractor, and extract it with 350W ultrasonic ultrasound for 45 minutes, then filter, collect the filter residue, and obtain Tenebrio molitor residue, and decompress the filtrate Distill to 25% of the volume of the stock solution to obtain the Tenebrio molitor extract oil, and set aside; then mix the above Tenebrio molitor re...
example 3
[0025] First weigh 1000g Tenebrio molitor, put it in a clean feeding box and starve it for 24 hours, then rinse it with deionized water for 3 times, then soak it in 800mL of 1.5% sodium chloride solution for 2 hours to get pretreated Tenebrio molitor, Transfer the pretreated Tenebrio molitor into a freeze-drying box, and freeze-dry it at -45°C for 12 hours to obtain freeze-inactivated dry Tenebrio molitor; then weigh 100 g of the above-mentioned freeze-inactivated dried Tenebrio molitor, put it into a tissue masher and pulverize it, Pass through a 60-mesh sieve, mix the sieved Tenebrio molitor powder with 1000mL methanol, put it into a Soxhlet extractor, and use 350W ultrasonic ultrasound to assist extraction for 50 minutes, then filter, collect the filter residue, and obtain Tenebrio molitor residue, and then decompress the filtrate Distill to 30% of the volume of the stock solution to obtain Tenebrio molitor extract oil, which is set aside; then mix the above Tenebrio molitor...
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