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Tissue engineering stent based on low-temperature rapid modeling and preparation method thereof

A tissue engineering scaffold, fast technology, applied in tissue regeneration, coating, medical science, etc., can solve the problems of excessive pore size and irregular shape of pure PLGA scaffolds, and achieve good biomechanical properties, high water content, and easy retention cell effect

Active Publication Date: 2017-05-17
杭州弘新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a composite excipient-based low-temperature rapid prototyping tissue engineering scaffold and its preparation for the defects of the existing fused deposition molding pure PLGA scaffolds with too large or too small apertures and irregular shapes. method

Method used

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  • Tissue engineering stent based on low-temperature rapid modeling and preparation method thereof
  • Tissue engineering stent based on low-temperature rapid modeling and preparation method thereof
  • Tissue engineering stent based on low-temperature rapid modeling and preparation method thereof

Examples

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preparation example Construction

[0055] Therefore, the preparation method of the tissue engineering scaffold based on low temperature rapid prototyping provided by the first embodiment of the present invention comprises the following steps:

[0056] (1) Dissolving PLGA in an organic solvent to obtain a PLGA solution, wherein the mass ratio of PLGA to organic solvent is 1:6-1:8 (for example, 1:6, 1:7 or 1:8). Preferably, the weight average molecular weight (Mw) of PLGA is 80000~200000gmol -1 (e.g. 80000, 100000, 120000, 150000 or 200000 gmol -1 ), more preferably 100000gmol -1 ). The low-temperature printing method of the present invention is especially suitable for the weight-average molecular weight (Mw) of 80,000-100,000 gmol -1 The PLGA material can avoid the change of material properties caused by high temperature heating. The organic solvent used in this step is preferably but not limited to: chloroform, dichloromethane, chloroform, dimethylsulfoxide, dimethylformamide or tetrahydrofuran.

[0057] (...

Embodiment 1

[0085] 1. Set the weight average molecular weight (Mw) to 100000gmol -1 PLGA and chloroform were mixed according to a mass ratio of 1:7 to obtain a PLGA solution.

[0086] 2. Add NaCl particles to the PLGA solution to obtain a printing paste, wherein the mass ratio of PLGA to NaCl particles is 1:1, and the NaCl particles are NaCl particles filtered through a 400-mesh screen.

[0087] 3. Put the printing slurry into the nozzle of the fused deposition modeling three-dimensional printer, and prepare for printing at room temperature such as 24°C and an air pressure of 200kPa.

[0088] 4. Set the fiber diameter to 300 μm and the fiber spacing to 300 μm, and print out a cylindrical scaffold body containing NaCl particles at a speed of 4 mm / s. The cylinder has a diameter of 9 mm and a thickness of 2 mm.

[0089] 5. Dry the stent body in an oven at 37°C for 48 hours.

[0090] 6. Soak the dried stent body in water for 48 hours, and dry to obtain the stent body after NaCl dissolves. ...

Embodiment 2 to 10

[0092] Examples 2 to 10 were carried out in substantially the same manner as Example 1 except for the contents of Table 1 below.

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Abstract

The invention relates to a tissue engineering stent based on low-temperature rapid modeling and a preparation method thereof. The preparation method comprises the following steps: mixing a polylactic acid-glycolic acid copolymer and an organic solvent in a mass ratio of 1: 6 to 1: 8 to prepare a PLGA (polylactic acid-glycolic acid) solution, and adding sodium chloride granules to obtain a printing slurry, wherein the mass ratio of the sodium chloride granules to the PLGA is 1: 2 to 2: 1; setting the fiber diameter to be 200-300 microns, setting the fiber spacing to be 300-350 microns, printing out a stent body containing the sodium chloride granules at a speed of 3-6mm / s, and removing the organic solvent and the sodium chloride to obtain the tissue engineering stent based on the low-temperature rapid modeling. According to the invention, through addition of an excipient, low-temperature printing of a PLGA material is achieved, and the defect of a too large or too small aperture size caused by high-temperature fused printing of a PLGA stent is overcome; the prepared PLGA stent is moderate in aperture size, can easily store cells, and has good biomechanical properties.

Description

technical field [0001] The invention relates to the technical field of biomaterials and tissue engineering, in particular to a tissue engineering scaffold based on low-temperature rapid prototyping and a preparation method thereof. Background technique [0002] Traditional tissue engineering scaffold preparation technologies include: fiber bonding technology, solution shallow casting / particle filtration technology, gas foaming technology, phase separation technology, emulsion freeze-drying technology, etc. However, these traditional techniques have common shortcomings: inconsistent pore size, irregular shape of pores, insufficient connectivity between pores, and poor repeatability. In contrast, as a rapid prototyping technology, the tissue engineering scaffold prepared by 3D printing technology has unique advantages: personalized scaffold, adjustable pore size, controllable porosity, good pore connectivity, and the shape of the hole can be designed , and construct complex s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/38A61L27/34A61L27/56A61L27/58C08J9/28C08J3/075
CPCA61L27/34A61L27/3834A61L27/3852A61L27/446A61L27/56A61L27/58A61L2430/06C08J3/075C08J9/28C08J2377/02C08L67/04C08L77/02
Inventor 余家阔王少杰朱钰方董亮
Owner 杭州弘新生物科技有限公司
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