Probiotic microcapsule with protein aggregate-polysaccharide as wall material and preparation method thereof

A technology of protein aggregates and microcapsules, which is applied in food forming, food ingredients as encapsulants, food science, etc., can solve the problems of natural protein instability and affect applications, and achieve high survival rate, good acid resistance and Effect of enteric solubility and uniform particle size distribution

Active Publication Date: 2019-03-12
SHENYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Proteins and polysaccharides are important components of food, and they are also widely used in food systems. Natural protein itself will produce some unfavorable properties and instability during processing, which will affect its application

Method used

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  • Probiotic microcapsule with protein aggregate-polysaccharide as wall material and preparation method thereof
  • Probiotic microcapsule with protein aggregate-polysaccharide as wall material and preparation method thereof
  • Probiotic microcapsule with protein aggregate-polysaccharide as wall material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Activation and cultivation of strains: the freeze-dried powder of lactic acid bacteria stored in the laboratory was inserted into an aqueous solution of 11% skim milk, and subjected to anaerobic constant temperature cultivation at 37° C. for 24 hours, and the skim milk solidified. Dilute 0.1mL curd with sterile water to prepare a suspension containing lactic acid bacteria. Separation of the bacterial suspension by streaking on a plate, anaerobic culture at 37°C for 24 hours, and continuous passage for 2-3 times in this way to fully activate it. Inoculate the activated lactic acid bacteria liquid into the MRS liquid medium, and after anaerobic constant temperature cultivation at 37°C to the logarithmic phase, centrifuge the obtained bacterial liquid at 4°C and 8000rmp for 10min, discard the supernatant, and collect the bacteria body.

[0023] (2) Configuration of microcapsule wall material solution: rice bran protein solution with a mass percentage of 15%

[0024] ...

Embodiment 2

[0028] Microcapsule preparation method is the same as example 1, change microcapsule wall material solution: rice bran protein aggregate solution

[0029] Preparation of rice bran protein aggregate solution: Dissolve a certain amount of rice bran protein in distilled water with a mass percentage of 15% and stir for 2 hours to ensure that the rice bran protein can be completely hydrolyzed, adjust the pH of the rice bran protein solution to 2, and collect the supernatant after centrifugation solution, and then heated and denatured in a water bath at 90°C for 2 hours, and then cooled rapidly in an ice bath to prevent the growth of aggregates to form a solution of rice bran protein aggregates.

[0030] The embedding rate of the obtained lactic acid bacteria microcapsules reaches 75% + 1.1%. 12 CFU / g.

Embodiment 3

[0032] Microcapsule preparation method is the same as example 1, change microcapsule wall material solution: the solution+polysaccharide solution of rice bran protein aggregate (gum arabic, dextran, chitosan)

[0033]Preparation of rice bran protein aggregate solution: Dissolve a certain amount of rice bran protein in distilled water with a mass percentage of 15%, stir for 2 hours to ensure that the rice bran protein can be completely hydrolyzed, adjust the pH of the rice bran protein solution to 2, and collect it after centrifugation The supernatant solution was heated and denatured in a water bath at 90°C for 2 hours, and after taking it out, it was quickly cooled in an ice bath to prevent the growth of aggregates, and the formed rice bran protein aggregate solution was ready for use;

[0034] The configuration of the microcapsule wall material solution: add 2% polysaccharide solution dropwise to the prepared rice bran protein aggregate solution according to the ratio (polysa...

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Abstract

The invention provides probiotic microcapsules with protein aggregate-polysaccharide as the wall material and a preparation method thereof. The preparation method is as follows: rice bran protein forms an aggregate and a polysaccharide solution at a low pH according to a certain volume ratio (1:1-10 : 1) Mix, add probiotic bacteria suspension and an appropriate amount of emulsifier (Tween-80) to the formed mixture and stir gently until a stable emulsion is formed, add 1% acetic acid under constant stirring The final pH of the solution is 3.8-4.0 in the mixed solution, and finally a curing agent (gallic acid) is added to solidify the microcapsules, and then washed with distilled water to finally obtain a probiotic microcapsule product. The present invention only needs to control the ratio of protein aggregates and polysaccharides and the crosslinking time to achieve the purpose of crosslinking. The prepared microcapsules of probiotics can improve the resistance to gastric acid, and finally release the probiotics in the intestinal tract. It is absorbed by the human body and has good storage properties.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a probiotic microcapsule product with rice bran protein aggregate-polysaccharide composite gel as wall material and a preparation method. Background technique [0002] Microcapsule technology is a technology that embeds solid, liquid or gaseous substances that are sensitive to the surrounding environment in capsules and can release them in a controlled manner in a specific environment. The size of microcapsule particles generally varies due to different processing techniques, and the particle size range is generally 0.1-1000um. Microcapsules are divided into two parts: wall material and core material. The material embedded in the microcapsule is called core material, and the material embedded outside is called wall material. Wall material is generally natural polymer material, semi-synthetic polymer material materials, fully synthetic polymer materials and inorganic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23P10/30A23L33/135A23L33/125A23L33/185
CPCA23V2002/00A23V2200/224A23V2250/5482
Inventor 肖志刚王霞高育哲王鹏李凡姝张一凡杨舒崔晓彤张文蕾
Owner SHENYANG NORMAL UNIV
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