Construction of a stem cell tumor targeting system bound to the surface of drug-loaded nanoparticles
A drug-loaded nanotechnology and tumor-targeting technology, applied in the fields of biomedical technology and nanomedicine, can solve the problems of little involvement and achieve the effect of easy release curve
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Embodiment 1
[0030] Embodiment 1: Synthesis and characterization of Bio-Chi
[0031] Dissolve 90 mg of chitosan in 1% hydrochloric acid solution and swell overnight, then dilute to 20 mL with 2-morpholine sodium sulfonate buffer solution with pH=5.5. Dissolve 120 mg of biotin, 84 mg of EDC, and 56 mg of NHS in a mixed solvent of 6 mL of DMSO and DMF, and stir at room temperature for 2 h. Add the activated biotin dropwise into the stirring chitosan solution, keep stirring for 24h, and filter to remove the insoluble matter. Place in a dialysis bag and dialyze with deionized water for 2 days, then freeze-dry.
[0032] Infrared spectrum analysis was carried out on the synthesized Bio-Chi, and the results showed that at 1635.6cm -1 The stretching vibration peak of the amide bond appears at 1531.9cm-1, and the stretching vibration peak of the amide bond of the biotin imidazolone ring appears at 1531.9cm-1, indicating that biotin and chitosan have been chemically bonded (attached figure 1 ). ...
Embodiment 2
[0033] Embodiment 2: Preparation and characterization of Cur / Bio-Chi
[0034] Weigh 10mg of Bio-Chi, dissolve in 1mL of 1% acetic acid solution, and swell overnight. With stirring at room temperature, 0.25 mL of 4 mg / mL Cur ethanol solution was added dropwise into the Bio-Chi solution. Stir continuously in a water bath at 50°C for 30min, sonicate with a 50W probe for 30min, dialyze for 6h to remove the organic solvent, pass through a 0.8μm hydrophilic microporous membrane, and dilute to the required concentration with PBS.
[0035] The particle size of Cur / Bio-Chi measured by a laser particle size analyzer was 360.2±2.6nm, and the potential was 9.6±1.9mV.
[0036] Take an appropriate amount of nanoparticle solution, add an equal amount of ethanol and vortex vigorously, measure the absorbance value at λ=431nm, calculate the Cur content in the nanoparticle solution according to the standard curve, and calculate the encapsulation efficiency according to the following formula: en...
Embodiment 3
[0037] Embodiment 3: the release profile of Cur / Bio-Chi
[0038]Take 0.4mL Cur / Bio-Chi, dilute it to 2mL with 10mMPBS7.4, place it in a dialysis bag (MWCO=14,000), tie both ends tightly, and immerse in 5mL of 10mMPBS containing 0.1% Tween 80 (pH=7.4, 6.5, 5.5 )middle. The release was performed at a constant temperature of 37° C. and a rotation speed of 100 rpm. At predetermined time points 2, 4, 8, 12, 24, 36, 48, 60, 72, 96, 120, 144, 168 h, the release medium was collected and replaced with 5 mL of fresh medium. Take 1 mL of the collected release medium, add 1 mL of ethanol, vortex vigorously for 5 min, measure the absorbance value at λ=431 nm with an ultraviolet-visible spectrophotometer, calculate the release amount, and draw the release curve.
[0039] The results showed that in the environment of pH=7.4, Cur / Bio-Chi could keep releasing Cur continuously and slowly for more than 7 days; complete, faster release (attached figure 2 ).
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