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Method for simultaneously measuring contents of gingko leaf extract and multiple components in preparation thereof

A technology of ginkgo biloba and extract, which is applied in the field of high-performance liquid chromatography detection for simultaneous determination of multi-component content in ginkgo biloba extract and its preparations, can solve the problems of not being able to detect flavonoids and phenolic acids at the same time, and achieve Low cost, good durability, easy to achieve effect

Active Publication Date: 2017-05-31
HEBEI SHINEWAY PHARMA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above two methods have fewer detectable components and cannot detect flavonoids and phenolic acids at the same time, so it is necessary to conduct more in-depth research and develop better detection techniques

Method used

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  • Method for simultaneously measuring contents of gingko leaf extract and multiple components in preparation thereof
  • Method for simultaneously measuring contents of gingko leaf extract and multiple components in preparation thereof
  • Method for simultaneously measuring contents of gingko leaf extract and multiple components in preparation thereof

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Experimental program
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Embodiment

[0037] 1. Chromatographic conditions

[0038] Chromatographic column: ACQUITY UPLC BEH C18 (1.7μm, 2.1×150mm);

[0039] Mobile phase B: methanol / acetonitrile (1:1);

[0040] Mobile phase A: ultrapure water (containing 0.1% formic acid);

[0041] Flow rate: 0.3mL / min;

[0042] Column temperature: 35°C;

[0043] Injection volume: 1μL;

[0044]Diode array detector: use 280nm ultraviolet wavelength to detect phenolic acid components, and simultaneously use 360nm ultraviolet wavelength to detect flavonoid glycoside components;

[0045] Analysis time: 35min;

[0046] Gradient elution method:

[0047]

[0048]

[0049] 2. Solution preparation

[0050] (1) Preparation of reference substance stock solution

[0051] Precisely weigh protocatechuic acid, cryptochlorogenic acid, smokylin, rutin, isoquercitrin, quercetin-3-O-glucosyl (1→2) rhamnoside, kaempferol-3-O -Rutinoside, astragalin, cosmoside, narciscin, isorhamnetin-3-O-β-D-glucoside, 3-O-{2-O-[6-O-(p-hydroxy -trans...

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Abstract

The invention relates to the field of medicament detection, and more specifically relates to a high performance liquid chromatography for simultaneously measuring the contents of gingko leaves (or extracts thereof) and multiple components in a preparation thereof. The method can be used for simultaneously measuring the contents of gingko leaves (or extracts thereof) as well as 14 flavonoid compounds and 2 phenolic acid compounds in a preparation thereof, and a new analysis method and basis are thus provided for quality control of commercial gingko leaf preparations.

Description

technical field [0001] The invention relates to the field of drug detection, more specifically, the invention relates to a high-performance liquid chromatography detection method for simultaneously determining the content of multiple components in ginkgo biloba extract and its preparation. Background technique [0002] Ginkgo is one of the oldest plants on earth. It is a plant of the Ginkgo family Ginkgo family. This tree species has strong vitality. It is not uncommon for a tree to be thousands of years old. It can be called the "old birthday star" in the plant world. It is recorded in "Compendium of Materia Medica" that Ginkgo biloba can invigorate the heart and nourish Qi, nourish the kidney and moisten the lungs, strengthen the brain and refresh the mind, and prolong life. Modern pharmacological studies have shown that Ginkgo biloba has the functions of scavenging oxygen free radicals, lowering blood fat, enhancing central nervous system function, regulating neurotransmi...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 常艳旭高秀梅田吉李晋王晖李志刚张纲郝福关秀伟
Owner HEBEI SHINEWAY PHARMA