Ultrasonic-microwave assisted enzymatic extraction of green tea polyphenol and application thereof in shower gel

A green tea polyphenol, microwave-assisted technology, which is applied to medical preparations containing active ingredients, cosmetic preparations, preparations for skin care, etc., can solve problems such as weak skin protection function and skin irritation, and achieve a sufficient source of raw materials. , Increase the extraction rate, the effect of high content

Inactive Publication Date: 2017-06-09
张仕英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Shower gel is one of the most frequently used daily necessities. Traditional shower gel formulas often use artificially synthesized detergents and additives. Although modified shower gels have strong decontamination capabilities, their skin protection functions are weak and may even cause certain skin irritations. irritant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preferred ultrasonic-microwave-assisted enzyme method of the present invention extracts green tea polyphenols and its application in shower gel, comprising the following steps:

[0041] 1. Ultrasonic-microwave-assisted enzymatic extraction of green tea polyphenols, the steps are as follows

[0042] (1) After washing the green tea leaves and drying them naturally, take the dried green tea leaves and pulverize them in a pulverizer to obtain a powder with a particle size of 45 mesh;

[0043] (2) After the green tea powder and water are prepared into a suspension at a mass volume ratio of 1:20, at room temperature, use an ultrasonic-microwave extraction instrument (ultrasonic power 45W, microwave power 70W) for 20 minutes, add 1% cellulase, ultrasonic-microwave assisted enzymatic hydrolysis for 30 minutes, and inactivated enzyme in a water bath at 100°C for 10 minutes;

[0044] (3) Centrifuge the enzyme-inactivated mixed solution at 5000r / min for 30 minutes while it is...

Embodiment 2

[0062]The preferred ultrasonic-microwave-assisted enzyme method of the present invention extracts green tea polyphenols and its application in shower gel, comprising the following steps:

[0063] 1. A method for extracting polyphenols from green tea by an ultrasonic-microwave-assisted enzymatic method, comprising the following steps:

[0064] (1) After washing the green tea leaves and drying them naturally, take the dried green tea leaves and pulverize them in a pulverizer to obtain a powder with a particle size of 63 mesh;

[0065] (2) After preparing a suspension of green tea powder and water at a mass volume ratio of 1:20, treat it with an ultrasonic-microwave extraction instrument (ultrasonic power 73W, microwave power 80W) at room temperature for 15 minutes, then add 2% cellulase, ultrasonic-microwave assisted enzymatic hydrolysis for 15 minutes, and inactivated enzyme in a water bath at 100°C for 10 minutes;

[0066] (3) Centrifuge the enzyme-inactivated mixture at 75,0...

Embodiment 3

[0084] The preferred ultrasonic-microwave-assisted enzyme method of the present invention extracts green tea polyphenols and its application in shower gel, comprising the following steps:

[0085] 1. A method for extracting polyphenols from green tea by an ultrasonic-microwave-assisted enzymatic method, comprising the following steps:

[0086] (1) After washing the green tea leaves and drying them naturally, take the dried green tea leaves and pulverize them in a pulverizer to obtain a powder with a particle size of 50 mesh;

[0087] (2) After the green tea powder and water are prepared into a suspension at a mass volume ratio of 1:20, at room temperature, use an ultrasonic-microwave extraction instrument (ultrasonic power 60W, microwave power 90W) for 10 minutes, add 3% cellulase, ultrasonic-microwave assisted enzymatic hydrolysis for 20 minutes, and inactivated enzyme in a water bath at 100°C for 20 minutes;

[0088] (3) Centrifuge the enzyme-inactivated mixture at 10,000 r...

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Abstract

The invention discloses an ultrasonic-microwave assisted enzymatic extraction method of green tea polyphenol. The method comprises the following steps: grinding weighed green tea leaves to obtain powder of which the particle size is 45 to 80 meshes, preparing the green tea powder into suspension liquid with water, at room temperature, using an ultrasonic-microwave extraction instrument of which the ultrasonic power is 45 to 60W, and the microwave power is 70 to 90W, after processing for 10 to 20 min, adding 1% to 3% of cellulase, carrying out ultrasound assisted enzymatic hydrolysis for 20 to 30 min, enzyme deactivation for 10 to 20 min under the conditions of a 100 DEG C-water bath, and centrifugation for 20 to 30 min at 5000 to 10000 r/min, performing pumping filtration after cooling, collecting filtrates, carrying out vacuum concentration to obtain a concentrated solution, then adding ethyl acetate to the concentrated solution to be extracted, and carrying out vacuum concentration, freeze concentration and freeze drying on organic phases, thereby being capable of obtaining the green tea polyphenol. The invention further provides shower gel containing the green tea polyphenol and a preparation method of the shower gel. The extraction method of the green tea polyphenol provided by the invention is simple to operate and high in extraction efficiency, furthermore, the green tea polyphenol extracted by the invention is applied to the shower gel, so that free radicals on the skin surface can be removed to play a good skin protective effect.

Description

technical field [0001] The invention relates to the extraction and preparation technology of green tea polyphenols, in particular to a method for extracting green tea polyphenols by an ultrasonic-microwave-assisted enzymatic method, and further relates to an application of the ultrasonic-microwave-assisted enzymatic method to extract green tea polyphenols in a shower gel. Background technique [0002] my country is a big producer of tea, and the waste of coarse and old tea produced in the processing of tea is serious, especially green tea. Green tea polyphenols are polyphenols in green tea leaves, which have anti-oxidation, anti-aging, anti-bacterial and anti-viral effects. When used in daily chemical products, they can play a role in sterilization and anti-bacteria, and can also inhibit skin itching and prevent peeling. Because the natural antioxidant effect can reduce the use of preservatives, it has been widely valued in daily chemical products in recent years. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/34A61K8/9789A61Q19/10A61Q19/08A61Q17/00A61K8/36A61K8/37A61K8/44A61K8/60A61K8/73A61K8/86
CPCA61K8/347A61K8/361A61K8/375A61K8/44A61K8/602A61K8/735A61K8/86A61K8/97A61K2800/82A61Q17/005A61Q19/08A61Q19/10
Inventor 张仕英
Owner 张仕英
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