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Method for extracting and continuously enriching polysaccharides from culture media for cordyceps militaris

A technology of Cordyceps militaris polysaccharides and Cordyceps polysaccharides, which is applied in the fields of medicine and health care and food processing, can solve the problems of easy damage to high-level structures, complex composition of crude polysaccharides, and difficulty in ensuring product quality and curative effect, so as to reduce energy consumption and protect Effects of activity, saving resources and cost

Inactive Publication Date: 2017-06-09
XUZHOU HONGYU AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional methods of extracting polysaccharides from Cordyceps polysaccharides, such as water extraction and alcohol precipitation, etc., can obtain crude polysaccharides with complex compositions and a wide range of molecular weights. Moreover, the high-level structures of polysaccharides are easily destroyed after the action of ethanol, and it is difficult to guarantee the quality of each batch of products. Therefore, it is of great significance to effectively extract and enrich the polysaccharides with biological activity to control the quality of Cordyceps polysaccharide products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Cut the Cordyceps militaris rice medium harvested from the same species into slices, and dry them in a vacuum drying oven at 70°C for 4 times for 2 hours each time until the water content of the medium slices is ≤5%; The prepared Cordyceps militaris rice culture medium slices with water content ≤5% are crushed with a hammer mill and passed through a 100-mesh sieve to obtain the Cordyceps militaris rice culture coarse powder, which is sent to the cyclone by the air stream to obtain the first level of airflow crushing After harvesting the isolate and the secondary isolate, use the 1:3 ratio of the material and the grinding ball for planetary ball milling for 1 hour, and pulverize 3 times to obtain the Cordyceps militaris rice medium superfine powder with uniform particle size, particle size distribution of 60μm, powder content ≤3%; After adding 10 times the proportion of water to the above medium superfine powder, first extract at 60℃ for 4h, then add cellulase, amylase and...

Embodiment 2

[0030] Cut the Cordyceps militaris rice culture medium of the same species harvested with Cordyceps militaris into slices, and dry them in a vacuum drying oven at 80℃ three times for 2 hours each time until the water content of the culture medium slices is ≤5%; The prepared Cordyceps militaris rice culture medium slices with water content ≤5% are crushed with a hammer mill and passed through a 100-mesh sieve to obtain the Cordyceps militaris rice culture coarse powder, which is sent to the cyclone by the air stream to obtain the first level of airflow crushing Separate and secondary separator, after harvesting, use a 1:2 ratio of materials and grinding balls to pulverize by planetary ball milling for 1 hour, and pulverize 3 times in total to obtain Cordyceps militaris rice medium superfine powder with uniform particle size, particle size distribution of 50μm, powder content ≤3%; After adding 15 times the water to the above medium superfine powder, first extract at 60°C for 3h, t...

Embodiment 3

[0032] Cut the Cordyceps militaris rice medium of the same species harvested with Cordyceps militaris into slices, and dry them in a vacuum drying oven at 90°C for 3 times for 1.5 hours each time, until the water content of the medium slices is ≤5%; Crush the prepared Cordyceps militaris rice culture medium with a water content of ≤5% with a hammer mill and pass through a 100-mesh sieve to obtain the Cordyceps militaris rice culture coarse powder, which is sent to the cyclone by air flow to obtain airflow crushing. After harvesting the first-level and second-level separators, use a 1:2 ratio of materials and grinding balls to pulverize by planetary ball milling for 1 hour, and pulverize 3 times in total to obtain the ultrafine powder of Cordyceps militaris rice medium with uniform particle size. The particle size distribution is 50μm. The content is less than or equal to 3%; after adding 20 times the proportion of water to the above medium superfine powder, first extract at 80℃ ...

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Abstract

The invention discloses a method for extracting and continuously enriching polysaccharides from culture media for cordyceps militaris. The method includes steps of drying the rice residual culture media for the cordyceps militaris; carrying out superfine grinding; carrying out enzymatic hydrolysis by the aid of biological multi-enzyme systems; carrying out centrifugal filtration; controlling backflow; carrying out multifunctional membrane concentration and enrichment; carrying out spray drying; carrying out qualitative analysis and quantitative analysis. The method has the advantages that the method for quickly, efficiently and economically separating the cordyceps polysaccharides is created; the cordyceps polysaccharides are prepared by the aid of functional membrane separation technologies and green extraction technologies such as enzyme processes, accordingly, the purity and the quality of the cordyceps polysaccharides can be guaranteed, the cordyceps polysaccharides can be quickly enriched and concentrated, and the biological activity of the cordyceps polysaccharides can be guaranteed; the waste culture media for the cordyceps militaris can be effectively utilized, and accordingly resources and the costs can be saved; the method is suitable for large-scale industrial production; the content of the cordyceps polysaccharides in polysaccharide concentrated liquid obtained by the aid of the method by means of separation can reach 80% at least, and the product recycle rate can reach 85% at least.

Description

Technical field [0001] The invention belongs to the technical fields of medicine and health care and food processing, and specifically belongs to a method for extracting and continuously enriching polysaccharides from a culture medium of Cordyceps militaris. Background technique [0002] Cordyceps militaris [Cordyceps militaris (Fr) Link.], also known as Cordyceps militaris, Cordyceps militaris, belongs to Ascomycotina, Sclerotium, Sphaeropsis, Claviceps, Cordyceps. It is a model species of Cordyceps and is widely distributed. It is recognized and accepted by scholars from all over the world. It has been bred into a complete pedestal under artificial conditions. Cordyceps militaris has a higher content of cordyceps polysaccharides and cordycepin and other effective ingredients, and its unique pharmacological effects It has attracted increasing attention from the pharmaceutical industry. Because of the above advantages of Cordyceps militaris, it has become the leader of the Chine...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/006C08B37/0003
Inventor 高兆建杨杰李同祥曹泽虹李勇沈彬彬曹建冬顾强
Owner XUZHOU HONGYU AGRI TECH
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