Target phospholipid covered fluorescent/nuclear magnetic dual-mode probe as well as preparation and application

A phospholipid-targeting, dual-modality technology, used in general/multifunctional contrast agents, preparations for in vivo experiments, pharmaceutical formulations, etc., can solve problems such as irreversible damage, and achieve stable properties, strong operability, Meet the effect of production and application

Inactive Publication Date: 2017-06-13
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And because the lesion is usually adjacent to the central nervous system, improper operation may lead to serious irreversible damage

Method used

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  • Target phospholipid covered fluorescent/nuclear magnetic dual-mode probe as well as preparation and application
  • Target phospholipid covered fluorescent/nuclear magnetic dual-mode probe as well as preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Dissolve the fluorescent (FITC) modified DSPE (FITC-DSPE), DSPE-PEG and the targeting DSPE (the sequence of the targeting peptide is CPVTRPPR) in 1 above in chloroform solution. Add zinc-doped Fe3O4 nanoparticles (Zn-Fe 3 O 4 ), making the phospholipids and Zn-Fe 3 O 4 The mass ratio is 2:1. Place the mixed solution in a round-bottomed flask, add an appropriate amount of Q water, and mix with ultrasound. Rotary evaporation of the above mixed solution at 70°C for 15 min. The zinc-doped ferroferric oxide nanoparticles are adsorbed on the bottom of the container by a magnet, and the supernatant is removed to remove the uncoated phospholipids, and finally a fluorescent / nuclear magnetic dual-mode probe coated with targeted phospholipids is obtained.

Embodiment 2

[0031] Dissolve the fluorescent (FITC) modified DSPE (FITC-DSPE), DSPE-PEG and the targeting DSPE (the sequence of the targeting peptide is CRPAKPAR) in 1 above in chloroform solution. Add zinc-doped Fe3O4 nanoparticles (Zn-Fe 3 O 4 ), making the phospholipids and Zn-Fe 3 O 4 The mass ratio is 3:1. Place the mixed solution in a round-bottomed flask, add an appropriate amount of Q water, and mix with ultrasound. Rotary evaporation of the above-mentioned mixed solution at 60°C for 20 min. The zinc-doped ferroferric oxide nanoparticles are adsorbed on the bottom of the container by a magnet, and the supernatant is removed to remove the uncoated phospholipids, and finally a fluorescent / nuclear magnetic dual-mode probe coated with targeted phospholipids is obtained.

Embodiment 3

[0033] Dissolve the fluorescent (FITC) modified DSPE (FITC-DSPE), DSPE-PEG and the targeted DSPE (the sequence of the targeting peptide is CRGERPPR) in the above 1 in 2.5 ml of chloroform solution. Add zinc-doped Fe3O4 nanoparticles (Zn-Fe 3 O 4 ) 2.5ml colloidal solution to make phospholipids and Zn-Fe 3 O 4 The mass ratio is 5:1. Place the mixed solution in a round bottom flask, add 5 ml of Q water, and mix well with ultrasound. Rotary evaporation of the above mixed solution at 80°C for 30 min. The zinc-doped ferroferric oxide nanoparticles are adsorbed on the bottom of the container by a magnet, and the supernatant is removed to remove the uncoated phospholipids, and finally a fluorescent / nuclear magnetic dual-mode probe coated with targeted phospholipids is obtained.

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Abstract

The invention relates to a target phospholipid covered fluorescent/nuclear magnetic dual-mode probe as well as preparation and application. The preparation comprises the following steps: dissolving distearoyl phosphoethanolamine-polyethylene glycol (DSPE-PEG) and a targeting molecule-DSPE (P-DSPE) into a chloroform or cyclohexane solution; adding zinc-doped ferroferric oxide nanoparticles (Zn-Fe3O4) to enable the mass ratio of phospholipid to the Zn-Fe3O4 to range from (1 to 1) to (10 to 1); putting a mixed solution into a round bottomed flask; adding a proper amount of ultrapure water and uniformly mixing by ultrasounds; carrying out rotary evaporation on the mixed solution at 40 DEG C to 80 DEG C for 10min to 30min; putting the zinc-doped ferroferric oxide nanoparticles adsorbed by magnetism into the bottom of a container; removing supernatant and removing uncovered phospholipid; finally, obtaining target phospholipid covered nano ferroferric oxide; preparing the target phospholipid covered fluorescent/nuclear magnetic dual-mode probe. The preparation method has a simple process and high operability, and can further meet production and application.

Description

Technical field [0001] The invention relates to a fluorescent / nuclear magnetic bimodal probe wrapped by targeting phospholipids, and its preparation and application. Specifically, it relates to phospholipid-coated oil-phase nano-Zn-Fe linked to targeting peptides 3 O 4 , At the same time, it has a preparation method of fluorescence / nuclear magnetic dual-mode imaging probe. The invention has application prospects in the field of drug loading. Background technique [0002] Brain tumors have a short survival period and high mortality rate, and seriously endanger human health. The current clinically used pharmaceutical preparations and nano-medicines are not effective. Therefore, early diagnosis of tumor is particularly important. To realize the safe and complete resection of the tumor in brain surgery, it mainly depends on the precise definition of the tumor boundary. Among them, as the main type of intracranial tumors, gliomas are poorly differentiated, have strong proliferation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/18A61K49/16A61K49/14
CPCA61K49/0002A61K49/0021A61K49/0056A61K49/0093A61K49/14A61K49/16A61K49/1866
Inventor 何丹农王萍祝闪闪朱君吴颖为陶晓峰金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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