Compound with inhibitory activity for bacteria, plant pathogenic fungi and cancer cells, preparation method and application thereof

A technology for plant pathogenic fungi and tumor cells, which is applied to compounds with inhibitory activity against bacteria, plant pathogenic fungi and tumor cells and the fields of their preparation and use, which can solve the problems of inability to obtain active compound monomers and complex components.

Inactive Publication Date: 2017-06-13
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Unfortunately, due to the low yield of microbial secondary metabolites, the content of pure biologically active compounds in the secondary metabolites of Streptomyces is very low, generally only reaching about 0.1% to 0.2% or lower , for the F2 component with relatively good antifungal activity, the composition of the F2 component was found to be complex after analytical HPLC analysis, so the active compound monomer in the F2 component could not be obtained

Method used

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  • Compound with inhibitory activity for bacteria, plant pathogenic fungi and cancer cells, preparation method and application thereof
  • Compound with inhibitory activity for bacteria, plant pathogenic fungi and cancer cells, preparation method and application thereof
  • Compound with inhibitory activity for bacteria, plant pathogenic fungi and cancer cells, preparation method and application thereof

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Embodiment Construction

[0021] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0022] The structural formula of a compound with inhibitory activity against bacteria, phytopathogenic fungi and tumor cells provided in this example is as follows: figure 1 Shown, wherein R is OH or H.

[0023] The compound is prepared by the following method: (1), insert the Streptomyces IIR21 strain into the seed culture medium, and cultivate it for 72 hours at 28°C to obtain the seed liquid; the seed culture medium is composed of the following components, each component And the mass percentage of each component is: glucose 0.1%, soluble starch 2.4%, soybean peptone 0.3%, yeast extract 0.5%, tryptone 0.5%, soybean powder 0.5%, sodium chloride 0.54%, potassium chloride 0.02%, magnesium chloride 0.24%, sodium sulfate 0.75%, calcium carbonate 0.4%, water balance;

[0024] (2), trans...

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Abstract

The invention provides a compound monomer extracted and separated from streptomycete IIR21 strain fermentation liquor. The monomer contains a 36-element ring polyhydroxy macrolides compound; three desoxy-sugars and 1,4-naphthoquinone are contained in a side chain; the compound monomer has excellent inhibitory activity for bacteria, plant pathogenic fungi and cancer cells. The invention also provides a preparation method for the compound. The compound can be used for preparing the drug for restraining bacteria, plant pathogenic fungi and cancer cells. The bacteria include micrococcus luteus; the fungi includes rhizoctonia solani, magnaporthe oryzae NO-1 strain, magnaporthe oryzae 168 strain, pythium germs, yellow fusarium wilt, Hegu fusarium wilt, brewer yeast and candida albicans; the cancer cells include human lung cancer cells, human breast cancer cells and colon cancer cells.

Description

technical field [0001] The invention provides a compound with inhibitory activity on bacteria, plant pathogenic fungi and tumor cells, and also provides a preparation method of the compound. Background technique [0002] Streptomyces IIR21 strain was isolated from Shennongjia area, Hubei Province, and was effective against Magnaporthe grisea NO-1, Magnaporthe grisea 168, Rhizoctonia solani, Botrytis cinerea, Fusarium aminearum, Fusarium culmorum, Verticillium dahliaekleb have certain antibacterial activity. [0003] Separation and purification of biologically active compounds from the fermentation broth of Streptomyces Ⅱ R21 strain is the premise of utilizing and developing the secondary metabolites of Streptomyces Ⅱ R21 strain. Through previous studies, it was determined that the effective antibacterial active ingredients in the fermentation broth of Streptomyces Ⅱ R21 strain were mainly concentrated in the medium polar ethyl acetate site. Fermented 5L fermented liquid su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/26C07H1/06A01N43/24A61K31/357A01P1/00A01P3/00A61P31/04A61P31/10A61P35/00
CPCA01N43/24C07H1/06C07H15/26
Inventor 李晓华马婷婷
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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