Kit capable of detecting human serum folate and metabolites thereof through tandem mass spectrometry

Abstract

The invention provides a kit capable of detecting human serum folate and metabolites thereof at the same time. The kit comprises a calibration material, a quality control material, an internal standard liquid, a dissociation agent, a dissociation agent diluent, a mobile phase A liquid, a mobile phase B liquid, a reconstitution fluid, a 96-pore microplate, an operating instruction and a packaging box for separating and intensively packaging these reagent bottles or tubes. The content of main folic acid in a human body is quantitatively detected by using a liquid chromatography-tandem mass spectrometry with high specificity and accuracy, the current folic acid state is reflected and the clinic detection quality is improved; an operation error caused by a corresponding self-made reagent can be avoided; and meanwhile, the operation convenience and the result stability can be improved.

Description

technical field [0001] The invention relates to quantitative detection of folic acid, a method for detecting folic acid and its metabolites in human serum by liquid chromatography tandem mass spectrometry, and a finished kit for detecting folic acid in serum. Background technique [0002] Folic acid (FA), also known as vitamin M and vitamin B9, is an important vitamin B family. Its chemical name is pteroyl glutamic acid, which is composed of p-aminobenzoic acid, pteridine, and glutamic acid residues. Natural folic acid exists mainly in the form of polyglutamic acid. The human body cannot directly synthesize folic acid, and it is mainly absorbed through food. The absorbed folic acid is mainly stored in the liver in the form of tetrahydrofolate, and also exists in other tissues such as the intestinal wall and bone marrow. Folic acid is reduced to physiologically active tetrahydrofolate under the action of reduced coenzyme II (NADPH), folate reductase and vitamin C, and then m...

AI Technical Summary

Problems solved by technology

[0004] The traditional clinical detection of folic acid substances mainly includes traditional methods such as microbial assay, isotope radioimmunoassay, ion capture method, time-resolved fluorescence method, chemiluminescence method and related kits, but the main problems are as follows: 1. These methods are all Therefore, the matrix effect cannot be ruled out and the instability of the compound is ignored, and the difference between different platforms is large, which is likely to cause different results on different platforms; second, this type of method does not accurately detect multiple indicators separately. Folic acid and its active folic acid metabolites can not better explain the metabolism of folic acid in the human body and the relationship between it and the health status of the body

In addition, there are currently technical methods that can detect genes related to folic acid metabolism, but this method detects related gene loci, which can be used for etiological analysis of folic acid metabolism ability, but cannot be used for clinical identification of patients with folic acid deficiency

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