Kit capable of detecting human serum folate and metabolites thereof through tandem mass spectrometry

A technology for detecting kits and serum folic acid, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of inconsistency, ignoring the instability of compounds, and large differences between different platforms, so as to simplify the experimental operation steps and facilitate clinical detection Fast and reduce the effect of operation error

Inactive Publication Date: 2017-06-13
GUANGZHOU DARUI BIOTECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The traditional clinical detection of folic acid substances mainly includes traditional methods such as microbial assay, isotope radioimmunoassay, ion capture method, time-resolved fluorescence method, chemiluminescence method and related kits, but the main problems are as follows: 1. These methods are all Therefore, the matrix effect cannot be ruled out and the instability of the compound is ignored, and the difference between different platforms is large, which is likely to cause different results on different platforms; second, this type of me

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  • Kit capable of detecting human serum folate and metabolites thereof through tandem mass spectrometry
  • Kit capable of detecting human serum folate and metabolites thereof through tandem mass spectrometry
  • Kit capable of detecting human serum folate and metabolites thereof through tandem mass spectrometry

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Embodiment 1

[0035] Embodiment 1: kit components

[0036]

[0037]

Embodiment 2

[0038] Embodiment 2, kit supporting operation method

[0039] 1. Preparation of reagent components:

[0040] (1) Calibrators (A, B, C, D, E, F) and quality controls (C1, C2): at room temperature, accurately measure 1000 μL of purified water into each bottle of calibrators or quality controls, to The freeze-dried powder is completely dissolved (not less than 15 minutes), and set aside.

[0041] (2) Internal standard: at room temperature, accurately measure 1000 μL of purified water into a bottle of internal standard until the lyophilized powder is completely dissolved (not less than 15 minutes), and set aside. In the embodiment of the present invention, the internal standard has included 13 C5 folic acid and d3 5-methyltetrahydrofolate are mixed internal standards and do not need to be prepared separately.

[0042] (3) Working fluid

[0043] a) Internal standard working solution: dilute the internal standard stock solution 200 times according to internal standard: mobile phas...

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Abstract

The invention provides a kit capable of detecting human serum folate and metabolites thereof at the same time. The kit comprises a calibration material, a quality control material, an internal standard liquid, a dissociation agent, a dissociation agent diluent, a mobile phase A liquid, a mobile phase B liquid, a reconstitution fluid, a 96-pore microplate, an operating instruction and a packaging box for separating and intensively packaging these reagent bottles or tubes. The content of main folic acid in a human body is quantitatively detected by using a liquid chromatography-tandem mass spectrometry with high specificity and accuracy, the current folic acid state is reflected and the clinic detection quality is improved; an operation error caused by a corresponding self-made reagent can be avoided; and meanwhile, the operation convenience and the result stability can be improved.

Description

technical field [0001] The invention relates to quantitative detection of folic acid, a method for detecting folic acid and its metabolites in human serum by liquid chromatography tandem mass spectrometry, and a finished kit for detecting folic acid in serum. Background technique [0002] Folic acid (FA), also known as vitamin M and vitamin B9, is an important vitamin B family. Its chemical name is pteroyl glutamic acid, which is composed of p-aminobenzoic acid, pteridine, and glutamic acid residues. Natural folic acid exists mainly in the form of polyglutamic acid. The human body cannot directly synthesize folic acid, and it is mainly absorbed through food. The absorbed folic acid is mainly stored in the liver in the form of tetrahydrofolate, and also exists in other tissues such as the intestinal wall and bone marrow. Folic acid is reduced to physiologically active tetrahydrofolate under the action of reduced coenzyme II (NADPH), folate reductase and vitamin C, and then m...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 吴英松董志宁周健伟龙允麟
Owner GUANGZHOU DARUI BIOTECH
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