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Serum amyloid protein A and procalcitonin two-in-one determination kit and preparation method

A technology of serum starch and procalcitonin, which is applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of strong interference and high intensity, and achieve the effects of high sensitivity, fast response and easy operation

Pending Publication Date: 2017-06-20
WEIHAI NEOPROBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the usual fluorescence measurement, because the test sample contains a variety of fluorescent components, the background fluorescence (scattered light caused by colloidal particles and solvent molecules in the sample and non-specific fluorescence emitted by proteins and other compounds in the serum) has a large intensity and interference. Strong, become the bottleneck of large-scale promotion of fluorescence analysis

Method used

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  • Serum amyloid protein A and procalcitonin two-in-one determination kit and preparation method
  • Serum amyloid protein A and procalcitonin two-in-one determination kit and preparation method
  • Serum amyloid protein A and procalcitonin two-in-one determination kit and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The various components of the test paper card in the serum amyloid A / procalcitonin two-in-one assay kit can be prepared by the following measures:

[0043] 1. Preparation of sample pad 2:

[0044] Soak the glass fiber membrane in the treatment solution containing 1.0% Triton X-100, 2.5% BSA, 0.15M Tris buffer, pH7.5, soak at 4°C for 4 hours, then place it in an oven and dry it at 37°C 2 hours. 2. Preparation of binding pad 3 for absorbing fluorescent microsphere-labeled antibody:

[0045] Soak the glass fiber membrane in 150mM Tris-HCL treatment solution (containing 1.0% Triton X-100, 2.5% BSA, pH7.4), soak at 4°C for 2 hours, then take it out of a 37°C oven and dry it for 4 hours, and set it aside. Place the glass fiber membrane on the Bio-DotXYZ3050 three-dimensional spraying platform, and use the Bio-Jet Quanti300 non-contact micro-quantitative nozzle to mix the two conjugated complexes of serum amyloid A and procalcitonin labeled with rare earth fluorescent micros...

Embodiment 2

[0054] Embodiment 2: accuracy test

[0055] Select the above-mentioned test paper card and fluorescent immunochromatography analyzer (model: NEO-007), the setting of the parameters of the fluorescent immune analyzer: after setting the process parameters of the test paper card on the fluorescent immune analyzer, take the above-mentioned assembled test paper card , respectively use 5, 20, 40, 60, 80, 100, 200mg / L SAA, 0.1, 1, 5, 10, 20, 30, 40ng / mL PCT calibrator, measure with test paper card, get each calibration The fluorescence intensity value of the product is input into the parameters of the analyzer to complete the setting of the parameters of the analyzer.

[0056] Main testing materials: clinical samples obtained from relevant hospitals, a total of 300 latex-enhanced immunoturbidimetric value samples, including 100 serum samples, 100 plasma samples, 100 whole blood samples, serum amyloid A / procalcitonin The content distribution intervals are: SAA: 5.00-200mg / L, PCT: 0.1...

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Abstract

The invention relates to a serum amyloid protein A and procalcitonin two-in-one determination kit and a preparation method. The kit is provided with a test paper card, and the test paper card is characterized by being provided with a PVC plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad from top to bottom in order. Specifically, rare earth Eu<3+> fluorescent microsphere respectivel labelled anti-serum amyloid protein A and anti-procalcitonin two monoclonal antibody-microsphere coupled compounds are adsorbed on the combination pad, the rare earth fluorescent microsphere has a diameter of 150nm, the rare earth fluorescent microsphere contains rare earth lanthanide Eu<3+>, is stable under a ground state, and can emit fluorescence with a wavelength of 615nm under the action of a 337nm excitation light source. The monoclonal antibodies are purified mixed monoclonal antibodies, and are respectively derived from monoclonal antibody cell lines directed at 2-6 different serum amyloid proteins A and procalcitonin epitopes. The kit has the advantages of simple operation, quick response, high sensitivity and strong specificity, etc.

Description

[0001] Technical field: [0002] The invention relates to the technical field of fluorescent immunochromatography in medical immunology, in particular to a rapid and accurate detection of serum amyloid A and procalcitonin in serum, plasma and whole blood samples, which are related to infection and inflammation. A two-in-one assay kit for the quantitative analysis of serum amyloid A and procalcitonin and its preparation method. [0003] Background technique: [0004] Serum amyloid A (SAA) belongs to the acute phase protein of the human body and exists at a low level in the blood. When the human body is infected by bacteria or viruses, it rises rapidly after inflammation or active lesions or tissue damage. It can rise rapidly during the acute phase of inflammation or infection and decline rapidly during the recovery phase of the disease. Studies have shown that elevated serum SAA can be detected in diseases such as bacteria, fungi, virus infection, atherosclerosis, cardiovascula...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/558G01N33/577G01N33/533G01N21/64
CPCG01N21/6428G01N33/533G01N33/558G01N33/577G01N33/68G01N2021/6439G01N2400/02G01N2400/16
Inventor 王有志王鹏浩李红江
Owner WEIHAI NEOPROBIO
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