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Method for producing biodiesel raw materials from Botryococcus braunii

A technology of botrytis branii and biodiesel, applied in the direction of microorganism-based methods, biochemical equipment and methods, biofuels, etc., can solve the problems of low bio-oil content of microalgae, achieve rapid growth, improve quality, and increase production Effect

Inactive Publication Date: 2017-07-07
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to provide a kind of method that produces biodiesel raw material with botrytis brachyphyllum, to overcome the defect that microalgae biological oil content is low in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] (1) Inoculate the triangular shaker flask containing Botrytis vulgaris into a sterile photobioreactor, and cultivate it under light in the sterilized bacteria medium; the photobioreactor is a bubbling closed reactor, including CO 2 Gas storage bottle, gas mixing device and gas flow meter; seed medium and culture conditions are as follows: sea salt 24g / L, CH 3 COONa 0.3g / L, NaNO 3 200mg / L, NaH 2 PO 4 50mg / L, Na 2 EDTA10mg / L, FeSO 4 ·7H 2 O 2.5mg / L, Vitamin B1 0.006mg / L, Vitamin B20.00005mg / L, the light source is a fluorescent lamp, the light intensity is 8Klux, the light quality is white light, the initial pH is 6.0, the ventilation intensity is 0.2VVM, CO 2 The concentration is 0.1%, the culture temperature is 23° C., cultured for 8 days until the logarithmic phase, and the seeds are collected for the next stage of fermentation.

[0057] (2) Cell collection adopts centrifugal collection technology. Under the condition of rotating speed of 4000rmp, centrifuge at ...

Embodiment 2

[0063] The steps are the same as in Example 1, but the photoautotrophic culture conditions are: the light quality is blue light, the light intensity is 9Klux, the initial pH is 6.5, the ventilation intensity is 0.6VVM, CO 2 The concentration is 1.5%, and the culture temperature is 25°C. The oil content reaches 36%, and the CN value is 53.0.

Embodiment 3

[0065] The steps are the same as in Example 1, but the photoautotrophic culture conditions are: the light quality is white light, the light intensity is 10Klux, the initial pH is 7.5, the ventilation intensity is 1.0VVM, CO 2 The concentration is 2.0%, and the culture temperature is 24°C. The oil content reaches 42%, and the CN value is 55.0.

[0066] Beneficial effects of the present invention:

[0067] The present invention provides a method for producing high-quality biodiesel raw material with Botrytis braziliana, in which Botrytis braziliana vulgaris is used as the algae species, using relatively mild light and light quality, sufficient nutrition, suitable nitrogen-to-phosphorus ratio, and suitable Temperature and other conditions can promote the rapid growth of microalgae cells, the accumulation of oil and the stability and improvement of oil quality;

[0068] The present invention can not only improve the oil output of Botrytis branii, but also effectively improve the q...

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Abstract

The invention discloses a method for producing biodiesel raw materials from Botryococcus braunii. The method includes the steps of (1), seed culture, (2), seed collection, (3), photoautotrophic culture, (4), microalgae harvest and (5) grease extraction. The method is used for culturing algae cells capable of controlling the oil content and the high-grease quality through introduction of indexes for representing the biodiesel quality, such as a cetane number and a ratio of unsaturated fatty acid to saturated fatty acid in terms of a culture condition and a culture technology of microalgae, and the grease content of the finally obtained algae cells is above 30%, a CN value is stabilized above 50, and the ratio of the unsaturated fatty acid to the saturated fatty acid between 1.5 and 2.5. The method is capable of effectively controlling the output and the quality of biodiesel from source raw materials and meets the application requirements of microalgae biodiesel industrialization.

Description

technical field [0001] The invention belongs to the fields of environmental protection and new technology energy, and in particular relates to a method for producing high-quality biodiesel raw material with botrytis branii. Background technique [0002] As a substitute for petrochemical diesel, microalgae biodiesel should meet the requirements for the use of petrochemical diesel, that is, its physical and chemical properties should be close to those of petrochemical diesel to ensure its fuel characteristics. The indicators to measure the properties of biodiesel mainly include: density, kinematic viscosity, flash point, distillation range, low temperature performance, carbon residue, ash, cetane number, methanol content, fat content, phosphorus content, glycerin content, iodine value and free fatty acid etc., in which the combustion performance of biodiesel was evaluated by cetane number. Combustion performance is one of the most important qualities of biodiesel, while cetan...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/12C12R1/89
CPCC12N1/12C12P7/6463Y02E50/10
Inventor 孙洪磊李建忠齐泮仑何皓雪晶张家仁孙立明李萌萌张佳侯丹
Owner PETROCHINA CO LTD
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