Rape pollen active peptide and preparation method of same

A technology of rapeseed pollen and active peptides, which is applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of waste of raw materials, human body absorption and utilization rate, nutrient-rich body utilization rate, etc., to achieve nutritional value and absorption utilization rate And the effects of improved food safety, good taste and natural color

Inactive Publication Date: 2017-07-14
赵德润 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the waste of raw materials and the low absorption and utilization rate of the human body in the extraction process of rape pollen active substances in the prior art, the present invention provides a preparation method of rape pollen active peptides, which is rich in bioactive peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1. 1. Preparation of rape pollen active peptide liquid: select 20 kg of fresh dried rape pollen of the year for ultrafine pulverization treatment; add water 10 times the weight of ultrafine pulverized rape pollen, dilute and soak for 30 minutes, stir evenly, and break evenly Adjust the PH value of rapeseed pollen after pulping to 6, add cellulase, pectinase, neutral protease, papain (1:1:1:2) for enzymatic hydrolysis, and the amount of enzyme added is 9500 per gram of rapeseed pollen Enzyme activity unit (u), temperature controlled at 50 degrees Celsius, enzymolysis for 4.5 hours; after enzymolysis, boiling water heat bath to inactivate the enzyme for 10 minutes, cooling and centrifuging, the centrifuge speed is about 9000rpm, and the rapeseed pollen active peptide liquid is obtained;

[0014] 2. Put the rape pollen active peptide solution prepared above into a stainless steel barrel and put it into a steam sterilizer, and sterilize it by steam and autoclave a...

Embodiment 2

[0017] Embodiment 2. 1. Preparation of rapeseed pollen active peptide liquid: select 25 kg of fresh dried rapeseed pollen of the year for ultrafine pulverization; add water 9 times the weight of ultrafine pulverized rapeseed pollen to dilute and soak for 30 minutes, stir evenly, and break evenly Adjust the PH value of rapeseed pollen after pulping to 4, add cellulase, pectinase, neutral protease, papain (1:1:1:2) for enzymatic hydrolysis, and the amount of enzyme added is 10000 per gram of rapeseed pollen Enzyme activity unit (u), temperature control 55 degrees Celsius, enzymolysis for 6 hours; after enzymolysis, boiling water heat bath to inactivate the enzyme for 15 minutes, cooling and centrifuging, the centrifuge speed is about 8000rpm, and the rapeseed pollen active peptide liquid is obtained.

[0018] 2. Put the rapeseed pollen active peptide solution prepared above into a stainless steel barrel and put it into a steam sterilizer, and sterilize it by steam and high pressu...

Embodiment 3 1

[0021] Example 3. 1. Preparation of rape pollen active peptide liquid: select 15 kg of fresh dried rape pollen of the year for ultrafine pulverization; add water 15 times the weight of ultrafine pulverized rape pollen, dilute and soak for 30 minutes, stir evenly, and break evenly Adjust the PH value of rapeseed pollen after pulping to 3.5, add cellulase, pectinase, neutral protease, papain (1:1:1:2) for enzymatic hydrolysis, and the enzyme dosage is 8000 pieces per gram of rapeseed pollen Enzyme activity unit (u), temperature controlled at 58 degrees Celsius, enzymolysis for 4.5 hours; after enzymolysis, boiling water heat bath to inactivate the enzyme for 12 minutes, cooling and centrifugation, the centrifuge speed is about 6000rpm, and the rape pollen active peptide liquid is obtained.

[0022] 2. Put the rapeseed pollen active peptide solution prepared above into a stainless steel barrel and put it into a steam sterilizer for 25 minutes at 115 degrees Celsius for steam autoc...

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Abstract

The invention relates to the field of traditional Chinese medicine and biotechnology and especially provides rape pollen active peptide and a preparation method of same. The preparation method includes the steps of: with rape pollen as a raw material, performing superfine pulverization, homogenation, enzymolysis, centrifugation, sterilization and ultra-filtration; and then according to production standard of oral liquids, fill-packaging the liquid to produce a dosage form of oral liquid; or according to the production standard of spray drying or freeze drying technology, processing the liquid into dry powder, and packaging the dry powder in cans to produce dosage forms of capsules and bottled freeze-dried powders. The rape pollen active peptide is greatly improved in nutritional value, absorption and utilization rate, and edible safety. The rape pollen active peptide is rich in bioactive peptides, and has the pharmacological activities of resisting arteriosclerosis, reducing blood fat and cholesterol, relieving pain, resisting irradiation, preventing and treating prostatitis, diabetes, anemia and constipation, and preventing and resisting cancer. The rape pollen active peptide is easy to digest and absorb, is rich in nutrition and is high in body utilization rate. The preparation method is rigorous and reasonable, has strong operability and is very suitable for large-scale production.

Description

technical field [0001] The invention relates to the fields of traditional Chinese medicine and biotechnology. In particular, a rape pollen active peptide prepared from rape pollen and a preparation method thereof. Background technique [0002] Rapeseed pollen is the most productive pollen in my country. It contains high flavonols. It has the functions of anti-arteriosclerosis, lowering blood fat and cholesterol, analgesia, anti-radiation, and treating constipation. It is effective in the treatment of prostatitis and can prevent prostate hypertrophy, Prostate dysfunction, but also in the treatment of varicose ulcers. Rapeseed pollen can enhance the strength of capillaries, lower blood lipids, and is effective in treating diabetes, anemia, constipation, and preventing and fighting cancer. [0003] Rapeseed pollen benefits the kidneys, strengthens the body, strengthens the waist, and contains high flavonols, which has the effects of anti-atherosclerosis, treating varicose ulce...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34A61K38/01A61P39/06A61P39/00A61P13/08A61P9/10A61P3/06A61P29/00A61P3/10A61P7/04A61P1/10A61P35/00
CPCA61K38/00C07K1/34C12P21/06
Inventor 赵德润赵振钧
Owner 赵德润
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