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Biodegradable microorganism sustained-release agent as well as preparation method and application thereof

A biodegradable and microbial technology, applied in the field of microbial sustained-release agents, can solve the problems of inferior physiological adaptability of strains, high frequency and amount of bacterial injection, and inability to grow dominant bacterial groups, etc., to achieve significant sustained-release effect, High embedding rate, avoiding the effect of utilization

Inactive Publication Date: 2017-07-18
浙江天韵生态环境工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main dosing method of water purification bacteria agent is to directly add it to the polluted water body or spray it to the bottom of the river. This method has the most outstanding effect in the application of aquaculture water. The application effect on the faster river course is not outstanding enough, and the frequency and amount of bacteria injection are very high, and the cost of manpower and materials is high
In addition, due to the huge volume of river sewage and the inferior physiological adaptability of artificially introduced bacteria, the artificial dosage of bacteria is usually far from changing the structure of the main indigenous bacteria, let alone growing into the dominant bacteria (according to Calculate the number of bacteria, the amount of artificially added bacteria is usually about 0.3‰ of the total amount of native bacteria)

Method used

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  • Biodegradable microorganism sustained-release agent as well as preparation method and application thereof
  • Biodegradable microorganism sustained-release agent as well as preparation method and application thereof
  • Biodegradable microorganism sustained-release agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of carbon source slow-release body: 20g sodium citrate, 10g sodium acetate, 20g polylactic acid, 30g polystyrene and 20g cod gum (food grade, Shandong Zhongke Biotechnology Co., Ltd.) were evenly mixed to obtain mixture. Then put the mixture into the barrel of the injection molding machine, and the injection molding temperature is 140° C. After one injection molding, the carbon source slow-release body is obtained after natural cooling at room temperature.

[0032] (2) Preparation of hydrogel bacterial agent carrier containing bacterial powder: Dissolve γ-polyglutamic acid in deionized water to prepare 200 mL aqueous solution of γ-polyglutamic acid (γ-PGA) with a concentration of 100 mg / mL , the temperature was adjusted to 40°C, and stirred at constant temperature for 10 hours to fully swell and dissolve. Then the γ-polyglutamic acid solution is placed in a three-necked flask, a mixture of 1.5g of ethylene glycol glycidyl ether / pentaerythritol and bacte...

Embodiment 2

[0040] (1) Preparation of carbon source slow-release body: 30 g of sodium citrate, 20 g of sodium acetate, 20 g of polylactic acid, 10 g of polystyrene and 5 g of cod gum were evenly mixed to obtain a mixture. Then put the mixture into the cylinder of the injection molding machine, the injection molding temperature is 160°C, after one injection molding, the carbon source slow-release body is obtained after natural cooling at room temperature.

[0041] (2) Preparation of bacteria powder-containing hydrogel bacterial agent carrier: Dissolve γ-polyglutamic acid in deionized water to prepare 200 mL aqueous solution of γ-polyglutamic acid with a concentration of 200 mg / mL, and adjust the temperature at 50 ℃, stirring at constant temperature for 14 hours to make it fully swell and dissolve. Then the γ-polyglutamic acid solution is placed in a three-necked flask, a mixture of 3.0g of ethylene glycol glycidyl ether / pentaerythritol and bacterium powder of 1.0g are added, and in the mix...

Embodiment 3

[0046] (1) Preparation of carbon source slow-release body: 36.0 g of sodium citrate, 27.0 g of sodium acetate, 30.0 g of polylactic acid, 5 g of polystyrene and 5 g of curd gum were uniformly mixed to obtain a mixture. Then put the mixture into the cylinder of the injection molding machine, and the injection molding temperature is 180° C. After one injection molding, the carbon source slow-release body is obtained after natural cooling at room temperature.

[0047] (2) Preparation of hydrogel bacterial agent carrier containing bacterial powder: Dissolve γ-polyglutamic acid in deionized water to prepare 200 mL aqueous solution of γ-polyglutamic acid with a concentration of 400 mg / mL, and adjust the temperature to 60 ℃, stirring at constant temperature for 16 hours to make it fully swell and dissolve. Then the γ-polyglutamic acid solution is placed in a three-necked flask, a mixture of 5.0 g of ethylene glycol glycidyl ether / pentaerythritol and 2.0 g of bacteria powder are added...

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Abstract

The invention relates to a biodegradable microorganism sustained-release agent. The biodegradable microorganism sustained-release agent is prepared from microorganism bacterium powder, a carbon-source sustained-release body and a hydrogel bacterium agent carrier, wherein the microorganism bacterium powder and the carbon-source sustained-release body are suspended in the hydrogel bacterium agent carrier; the mass ratio of the carbon-source sustained-release body to the hydrogel bacterium agent carrier is 1 to (1 to 10000); the invention further relates to a preparation method and application of the microorganism sustained-release agent. The microorganism sustained-release agent provided by the invention has a remarkable sustained-release effect, the embedding rate of the microorganism bacterium powder is high and the sustained-release effect of the microorganism bacterium powder is remarkable; furthermore, the preparation method is simple and secondary pollution is effectively avoided; controllable release of a carbon source can be realized; the biodegradable microorganism sustained-release agent is applied to sewage management and waste of the carbon source, caused by flowing of a water body, can be effectively avoided through sustained-release of the carbon source, so that the utilization efficiency of microorganisms on the carbon source is improved and the nitrogen removal efficiency is improved; the ammonia-nitrogen reducing rate can reach 80 percent or more and the reducing rate of total nitrogen can reach 20 percent; a relatively good quality effect is realized.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a biodegradable microbial slow-release agent and its preparation method and application. Background technique [0002] Traditional river management methods are mainly based on water conservancy construction, such as dredging, water exchange, etc. These methods are not only time-consuming and laborious, but also often have insufficient long-lasting effects, and have not fundamentally solved the problem of the river. In recent years, bioremediation (especially microbial enhanced restoration) technology has outstanding performance in terms of environmental friendliness and long-term effectiveness of water treatment. Commonly used bioremediation technologies include adding microbial agents to sewage, artificial reoxygenation, water cycle, Side stream purification, planting aquatic plants, stocking aquatic animals, etc. are used to improve the self-purification ability of water...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C02F3/34C12R1/125C12R1/10C02F101/16
CPCC02F3/34C02F2101/16C12N11/04C12N11/10
Inventor 孙燕任红星刘荣华范娟
Owner 浙江天韵生态环境工程有限公司
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