Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of carbon nanocage fluorescent probe and application thereof in Fe<3+> detection

The technology of fluorescent probe and carbon nano-cages is applied in the field of preparation and application of fluorescent probe, which can solve the problems of difficult operation and complicated preparation method, and achieve the effects of low cost, simple and convenient operation, and simple and controllable preparation process.

Active Publication Date: 2017-07-21
DALIAN POLYTECHNIC UNIVERSITY
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the preparation methods of carbon nanocages mainly include chemical vapor deposition, template method and plasma polymerization method, etc. The preparation methods are complicated and difficult to operate.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of carbon nanocage fluorescent probe and application thereof in Fe&lt;3+&gt; detection
  • Preparation method of carbon nanocage fluorescent probe and application thereof in Fe&lt;3+&gt; detection
  • Preparation method of carbon nanocage fluorescent probe and application thereof in Fe&lt;3+&gt; detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Preparation of carbon nanocage fluorescent probe

[0046]The conger eel baked at 180°C for 30 minutes was soaked in absolute ethanol with 1ml of ethanol per gram of fish for 15 hours to roughly extract the carbon nanocages; until the ethanol was completely removed by rotary evaporation, three Chloromethane was redissolved, extracted three times, and degreased until the aqueous phase solution was clear and transparent. The crude aqueous phase solution was subjected to G25 dextran gel column chromatography, and the collected fluorescence intensity was higher than 80 (excitation wavelength = 330nm, emission wavelength = 390nm ) part, freeze-dried to obtain a powdery substance.

[0047] It can be detected by a transmission electron microscope that it has a hollow carbon nanocage structure with multiple ring-shaped shell layers and a diameter of less than 100 nm.

[0048] The carbon nanocage fluorescence yield is measured by a known formula, the formula is: Φ ...

Embodiment 2

[0051] Embodiment 2: Preparation of carbon nanocage fluorescent probe

[0052] The conger eel that had been baked at 280°C for 15 minutes was soaked in absolute ethanol with 1ml of ethanol per gram of fish for 12 hours to roughly extract the carbon nanocages; until the ethanol was completely removed by rotary evaporation, three Chloromethane was redissolved, extracted three times, and degreased until the aqueous phase solution was clear and transparent. The crude aqueous phase solution was subjected to G25 dextran gel column chromatography, and the collected fluorescence intensity was higher than 80 (excitation wavelength = 330nm, emission wavelength = 390nm ) part, freeze-dried to obtain a powdery substance.

[0053] It can be detected by a transmission electron microscope that it has a hollow carbon nanocage structure with multiple ring-shaped shell layers and a diameter of less than 100 nm.

[0054] The carbon nanocage fluorescence yield is measured by a known formula, the...

Embodiment 3

[0057] Embodiment 3: Preparation of carbon nanocage fluorescent probe

[0058] Soak the whole turbot roasted at 230°C for 30 minutes in absolute ethanol, leaching with 1ml of ethanol per gram of fish for 12 hours, and extract the carbon nanocages roughly; until the ethanol is completely removed, use Trichloromethane redissolution, extraction three times, degreasing, until the aqueous phase solution is clear and transparent, the crude aqueous phase solution is subjected to G25 dextran gel column chromatography, and the collected fluorescence intensity is higher than 80 (excitation wavelength=330nm, emission wavelength= 390nm) part was lyophilized to obtain a powdery substance.

[0059] It can be detected by a transmission electron microscope that it has a hollow carbon nanocage structure with multiple ring-shaped shell layers and a diameter of less than 100 nm.

[0060] The carbon nanocage fluorescence yield is measured by a known formula, the formula is: Φ 1 = Φ 2 I 1 A 2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
wavelengthaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a preparation method of a carbon nanocage fluorescent probe. The specific steps include: taking fish as the raw material for baking, soaking the baked fish in anhydrous ethanol and performing extraction; collecting the liquid phase after extraction treatment, and performing rotary evaporation to remove ethanol; conducting trichloromethane redissolving and extraction for degreasing, carrying out extraction repeatedly until the aqueous phase becomes clear and transparent, collecting the aqueous phase, performing chromatography on a G25 sephadex column, collecting the part with fluorescence intensity of greater than or equal to 80, excitation wavelength of 330nm and emission wavelength of 390nm, and performing freeze-drying, thus obtaining a powdery substance, i.e. the carbon nanocage fluorescent probe. The invention also provides application of the carbon nanocage fluorescent probe in Fe<3+> detection. The carbon nanocage fluorescent probe prepared by the method provided by the invention has a hollow structure and has a multi-layer annular shell layer, and has the advantages of high detection sensitivity and stability, long fluorescence lifetime, and high quantum yield. The Fe<3+> concentration detection method provided by the invention has a minimum detection limit up to 3.96 microM.

Description

technical field [0001] The invention relates to the technical field of preparation and application of fluorescent probes, in particular to a preparation method of a carbon nanocage fluorescent probe and its application in Fe 3+ application in detection. Background technique [0002] Carbon nanocages (Carbon nanonages, CNCs) are hollow carbon nanomaterials covered by graphite layers. Most of the CNC surface structure is similar to porous carbon, which has a unique hollow pore structure, so its physical and chemical properties are relatively unique, such as high specific surface area, strong corrosion resistance, good electrical conductivity, good biocompatibility, etc., and can be used in many fields , including drug carriers, data storage, environmental purification, capacitor materials, lithium batteries, hydrogen storage materials, protective proteins and catalytic enzymes, etc. So far, the preparation methods of carbon nanocages mainly include chemical vapor deposition,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/15C09K11/65G01N21/64
CPCC01P2004/04C01P2004/64C09K11/65G01N21/6428
Inventor 谭明乾毕景然王海涛宋玉昆丛爽宋勋禹李加齐曹林
Owner DALIAN POLYTECHNIC UNIVERSITY