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Heparin sodium color removal method

A technology of sodium heparin and solution, which is applied in the field of color removal of sodium heparin, can solve the problems of lower product quality and yield, structural damage of heparin sodium, and ineffective removal, etc., and achieve the goal of improving product quality, reducing dosage, and reducing damage Effect

Inactive Publication Date: 2017-07-21
GUANGYUAN HAIPENG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the raw material for the production of heparin sodium in China is mainly pig small intestine. The color of heparin sodium is directly related to protein impurities and iron in blood. These impurities will form metal bridges with heparin sodium. Use ordinary protein removal methods or directly add metal The removal effect of the complexing agent is not obvious, leading to the steps of direct color removal in the refining process, such as the commonly used potassium permanganate oxidation, hydrogen peroxide oxidation or the combination of the two, the use of oxidants is relatively large, which is harmful to heparin The structure of sodium is destroyed, reducing the quality and yield of the product

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0024] The present embodiment provides a method for decolorizing sodium heparin, comprising the following steps:

[0025] Step 1. Dissolution: first add water to the dissolving container, open the steam valve, heat, turn on the stirring, put in the crude heparin sodium to dissolve, control the temperature at 45°C during the dissolution process, and obtain the feed liquid after the crude heparin sodium is completely dissolved. Finally, the concentration of heparin sodium solution in the feed liquid is 4200IU / ml;

[0026] Step 2. Add sodium nitrite with a mass concentration of 0.3% to the feed liquid obtained in step 1 and stir for 20 minutes, then adjust the pH of the solution to 9.5 to 10.0 to obtain a degradation solution, add sodium borohydride to the degradation solution and stir for 2 hours, and adjust the pH of the solution to 3.4 , stirred for 20 minutes, irradiated the degradation solution with ultraviolet light, adjusted the pH of the solution to 7.1, and adjusted the ...

Embodiment 2

[0033] The present embodiment provides a method for decolorizing sodium heparin, comprising the following steps:

[0034] Step 1. Dissolution: first add water to the dissolving container, open the steam valve, heat, turn on the stirring, put in the crude heparin sodium to dissolve, control the temperature at 60°C during the dissolution process, and obtain the feed liquid after the crude heparin sodium is completely dissolved. Finally, the concentration of heparin sodium solution in the feed liquid is 8500IU / ml;

[0035] Step 2. Add sodium nitrite with a mass concentration of 1.5% to the feed solution obtained in step 1 and stir for 20 minutes, adjust the pH of the solution to 10.0 to obtain a degradation solution, add sodium borohydride to the degradation solution and stir for 5 hours, adjust the pH of the solution to 3.6, and stir For 20 minutes, irradiate the degradation solution with ultraviolet light, adjust the pH of the solution to 7.6, and adjust the salinity of the fee...

Embodiment 3

[0042] The present embodiment provides a method for decolorizing sodium heparin, comprising the following steps:

[0043] Step 1. Dissolution: first add water to the dissolving container, open the steam valve, heat, turn on the stirring, put in the crude heparin sodium to dissolve, control the temperature at 50°C during the dissolution process, and obtain the feed liquid after the crude heparin sodium is completely dissolved. Finally, the concentration of heparin sodium solution in the feed liquid is 8000IU / ml;

[0044] Step 2. Add sodium nitrite to the feed solution obtained in step 1 and stir for 20 minutes, adjust the pH of the solution to 9.8 to obtain a degradation solution, add sodium borohydride to the degradation solution and stir for 4 hours, adjust the pH of the solution to 3.5, stir for 20 minutes, and degrade by ultraviolet light irradiation liquid, adjust the pH of the solution to 7.4, and adjust the salinity of the feed liquid to 2.7°;

[0045] Step 3, adding di...

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Abstract

The invention discloses a heparin sodium color removal method and relates to the technical field of heparin sodium purification method. The heparin sodium color removal method comprises the following steps that water is added for dissolution, sodium nitrite is added to regulate the PH value and salinity of the solution, the solution is degraded through ultraviolet lamp irradiation, diatomite having the concentration of 1% is added, then filtration is performed, 0.45-0.8% of ethylenediamine tetraacetic acid and 2.5% of sodium chloride solid are added in the filtrate, then ethanol with the alcoholic strength of 75 degrees or above is added, suction filtration, dewatering and drying are performed to obtain a heparin sodium product. Metal bridges between heparin sodium and protein impurities and an iron element can be effectively destroyed, a heparin sodium structure is also not destroyed, and the heparin sodium is small in activity damage, stable in quality and high in efficiency.

Description

technical field [0001] The invention relates to the technical field of heparin sodium purification methods, in particular to a heparin sodium decolorization method. Background technique [0002] Heparin Sodium is a mucopolysaccharide sulfate anticoagulant. Heparin sodium is the sodium salt of aminodextran sulfate extracted from the intestinal mucosa of pigs or cattle, and belongs to mucopolysaccharides. Studies in recent years have proved that heparin sodium also has the effect of lowering blood lipids. [0003] Heparin sodium is the most widely used anticoagulant clinically, and it appears in the form of complexes with proteins in vivo. This complex has no anticoagulant activity, and it can only exhibit anticoagulant activity if it is isolated alone. Heparin sodium is widely distributed in the biological world, and exists in higher mammals, such as lung tissue and intestinal tissue of pigs, cattle, and sheep. Common separation methods for heparin sodium include enzymati...

Claims

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Application Information

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IPC IPC(8): C08B37/10
CPCC08B37/0075
Inventor 李海生
Owner GUANGYUAN HAIPENG BIOLOGICAL TECH
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