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Oligopeptide and preparation method and application thereof

An oligopeptide and reaction solution technology, applied in the direction of peptide, tripeptide components, pharmaceutical formulations, etc., to achieve the effect of good premature ejaculation and premature ejaculation improvement

Inactive Publication Date: 2017-07-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report of peptide substances with aphrodisiac effect

Method used

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  • Oligopeptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Inoculate Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SWJS22 (General Microorganism Center of China Microbiological Culture Collection Management Committee, preservation number is CGMCC No.8425) with 1% inoculum in the fermentation medium (the composition of the fermentation medium is: 4 % soybean meal, 2% bran, 2% corn flour, 92% water), ferment and cultivate at 37-39°C for 30h. The fermentation broth was centrifuged at 4°C and 10,000 r / min at high speed for 10 minutes, and the supernatant was filtered to obtain the crude glutaminase enzyme solution.

[0019] Dissolve 400mM glutamine and 400mM arginine in 100g water, adjust the pH value of the solution to 10.0, add 1g glutaminase crude enzyme solution, react at 25°C for 12h, and then adjust the pH to 7.0 with 4mol / L hydrochloric acid Afterwards, the enzyme was extinguished at 90° C. for 10 minutes, the enzymatic reaction product was concentrated, and freeze-dried to obtain a reaction liquid containing oligo...

Embodiment 2

[0022] 1. Toxicity test

[0023] In this experiment, healthy SD rats were used, and the oligopeptide γ-Glu-γ-Glu-Arg prepared in Example 1 was orally administered in a dose gradient of 10 mg / kg and 30 mg / kg, and administered continuously for 30 days. During the period and within 1 week after stopping the drug, there were no significant effects on the animal growth status, activity diet, hematology, blood biochemistry, organ tissue structure, and urine routine, which indicated that the clinical drug was safe and reliable.

[0024] 2. Mice mating test

[0025] Mouse mating test. Take 25 Kunming mice in each group, including 5 males and 20 females. Each group was housed in 5 mouse cages. Each male mouse was housed in the same cage as 4 female mice. They were then divided into blank group (distilled water), oligopeptide γ-Glu-γ-Glu-Arg (10 mg / kg, based on mouse body weight) and arginine group. The male rats in each group were intragastrically administered, once in the morning...

Embodiment 3

[0035] 77 outpatients with ED were randomly divided into groups, including 38 cases in the treatment group and 39 cases in the control group; there was no significant difference between the two groups in age, course of disease, erectile function scores, and severity of illness, which were comparable. The diagnostic criteria refer to the international diagnostic principles of ED, that is, under sexual stimulation, the man continuously achieves or maintains a penile erection with sufficient hardness to complete satisfactory sexual intercourse, and the course of the disease is more than 3 months, and the International Erectile Function Score Questionnaire (IIEF-5) ≤ 21 points, diagnosed as ED.

[0036] Exclusion criteria (1) Those with diabetes; (2) Those with serious heart, liver, kidney, hematopoietic system diseases; (3) Those with obvious hyperplasia of the prostate or those with PSA > 4ug / ml; ( 4) Mentally ill patients; (5) Those who have recently taken other related drugs; ...

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Abstract

The invention discloses oligopeptide and a preparation method and application thereof. An amino acid sequence of the oligopeptide is gamma-Glu-gamma-Glu-Arg, and the oligopeptide has the advantages that the safety is high, any toxic or side effect is avoided, and the function of relieving erectile dysfunction and prospermia is better. The preparation method specifically comprises the following steps of mixing glutamine and arginine, dissolving into water, adding glutaminase or gamma-glutaminase of which the mass accounts for 0.001 to 0.4%(w / w) of the solution, using 10mol / l sodium hydroxide solution to adjust the pH (potential of hydrogen) value of the solution to 7.0 to 10.0, reacting for 3 to 12h at the temperature of 25 to 50 DEG C, using 4mol / l hydrochloric acid to adjust the pH value to 7 to 8, and deactivating enzyme for 10min at the temperature of 90 DEG C.

Description

technical field [0001] The invention relates to a peptide with physiological activity and a preparation method thereof, in particular to a gamma-glutamyl peptide mixture with an aphrodisiac effect and a preparation method thereof. Background technique [0002] Erectile dysfunction (ED) is also known as impotence. It generally refers to the inability of the penis to achieve an erection in most cases, or the inability to maintain an erection and perform sexual intercourse even though it can be erected. It is the most common male sexual dysfunction in clinical practice. the trend of. Especially in modern life, people's fast pace of life, high mental stress, overeating, partial eclipse and alcoholism, etc., can easily cause male sexual dysfunction and impotence symptoms. According to statistics, 52% of men aged 40 to 70 suffer from ED in varying degrees. Its treatment can be divided into non-invasive and invasive treatment two categories, non-invasive treatment refers to psyc...

Claims

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Application Information

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IPC IPC(8): C07K5/027A61K38/06A61P15/10A23L33/18
CPCC07K5/0215A61K38/00
Inventor 崔春杨娟
Owner SOUTH CHINA UNIV OF TECH
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