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A kind of microbial enzymolysis soybean protein

A soybean protein and microbial enzyme technology, applied in animal feed, animal feed, additional food elements, etc., can solve the problems of lack of industrialized production equipment and supporting facilities, reduction of nutrient digestion and utilization rate, and inability to monitor production process and results. , to inhibit the growth of harmful bacteria, improve the micro-ecological balance, and improve the utilization of protein

Active Publication Date: 2018-06-19
智农健康产业科技(广东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are two problems when soybean meal is directly used as feed raw material. First, the high crude protein content leads to insufficient digestibility. Second, soybean meal contains more than 10 kinds of anti-nutritional factors, which can inhibit the digestion and absorption process in different ways. lead to decreased digestion and utilization of nutrients
[0004] After exploration and practice in recent years, so far there are still a lot of thorny problems in soybean protein fermentation technology. So far, there is no standard production process, no standard industrial production equipment and supporting facilities, and high temperature drying and inactivation, resulting in the production process and results. Monitoring, marketed products are mixed, seriously affecting the quality of soybean protein products

Method used

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  • A kind of microbial enzymolysis soybean protein

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Experimental program
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Effect test

Embodiment 1

[0050] Bacillus subtilis and Bacillus coagulans were multiplied and cultured at a ratio of 1:1. Add bean curd powder and diatomaceous earth to the cultured liquid bacteria, and dry at 35°C for 50 minutes at a low temperature to prepare a microbial bacterial agent. The microbial inoculum contains 10% soybean hull powder and 90% diatomaceous earth by weight ratio, and the total content of Bacillus subtilis and Bacillus coagulans contained therein is 1*10 8 CFU / g.

[0051] The feed raw material is fermented in a feed fermentation mixer by using a microbial agent to prepare microbial concentrated enzyme protein. The specific process is as follows:

[0052] Put 1000kg of soybean meal into the feed fermentation mixer that has been started, add 700kg of filtered water and stir for 3 minutes, so that the moisture content of the feed raw materials in the feed fermentation mixer is 50%; add 7.5kg of microbial agents into the above feed fermentation mixer. The frequency is 50HZ, the r...

Embodiment 2

[0056] Bacillus subtilis, Bacillus coagulans and yeast were multiplied and cultured at a ratio of 1:1:1. Add bean curd powder and diatomaceous earth to the cultured liquid bacteria, and dry at 37° C. for 45 minutes at a low temperature to prepare the first microbial agent. The first microbial agent contains 15% bean curd powder and 85% diatomaceous earth by weight ratio, and the total content of Bacillus subtilis, Bacillus coagulans and yeast contained therein is 1*10 9 CFU / g.

[0057]Bacillus subtilis and Bacillus coagulans were multiplied and cultured at a ratio of 1:3:1. Soybean skin powder and diatomaceous earth were added to the cultured liquid bacteria and dried at a low temperature of 37° C. for 45 minutes to prepare the second microbial bacterial agent. The second microbial inoculant contains 15% bean curd powder and 85% diatomaceous earth by weight ratio, and the total content of Bacillus subtilis, Bacillus coagulans and yeast contained therein is 1*10 9 CFU / g.

...

Embodiment 3

[0063] Soybean skin powder and diatomaceous earth were added to the bacterial solution containing Bacillus subtilis and dried at a low temperature of 40° C. for 50 minutes to prepare the first microbial bacterial agent. The first microbial agent contains 10% bean curd powder and 90% diatomaceous earth by weight ratio, wherein the total content of Bacillus subtilis is 1*10 8 CFU / g.

[0064] Add soybean hull powder and diatomaceous earth to the bacterial solution containing Bacillus coagulans, and dry at 40° C. for 50 minutes at low temperature to prepare the second microbial bacterial agent. The second microbial agent contains 10% bean curd powder and 90% diatomaceous earth by weight ratio, and the total content of Bacillus coagulans contained therein is 1*10 8 CFU / g.

[0065] The feed raw material is fermented in a feed fermentation mixer by using a microbial agent to prepare microbial concentrated enzyme protein. The specific process is as follows:

[0066] Put 500kg of r...

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Abstract

The invention relates to the technical field of feed processing and manufacturing, and discloses a microbial enzyme hydrolyzed soybean protein. The microbial l enzyme hydrolyzed soybean protein is prepared through fermentation and cold granulation of 80-90 parts by weight of soybean meal and 1-10 parts by weight of microbial concentrated enzyme proteins. The fermentation and cold granulation technology comprises the following steps: crushing and mixing the above raw materials, adding the obtained mixture into a cold granulation device, adjusting the ring mold and the compression ratio matched with the cold granulation device to carry out granulation, rising the temperature of the raw materials to 60-70 DEG C in 3-15 min, cooling the heated raw materials, and carrying out crushing and wall breaking to obtain the microbial enzyme hydrolyzed soybean protein. The microbial enzyme hydrolyzed soybean protein is prepared through the fermentation and cold granulation device, the feed raw materials and the microbial concentrated enzyme proteins are organically combined during the granulation, and mutual friction heating of a machine and the materials is directly used to provide fermentation conditions for microbial value adding and changing of macromolecular substances into micro-molecular substances, so the functionalization of the raw materials is realized.

Description

technical field [0001] The invention relates to the technical field of feed processing application, in particular to a microbial enzymatic hydrolysis of soybean protein. Background technique [0002] Worldwide, with the continuous development of the aquaculture industry, the demand for feed is increasing day by day. For a long time, the protein raw material of the feed is mainly fish meal, supplemented by bone meal, poultry meal, plasma protein meal and so on. However, fish meal is a scarce resource, and bone meal, poultry meal, plasma protein meal, etc. are restricted due to public health reasons, so that animal protein is far from meeting the growing needs of the world's feed. [0003] The source of plant protein is very rich, and among the plant proteins, soybean has the largest output. Soybean meal is a by-product obtained after extracting soybean oil from soybean. Soybean meal is rich in nutrients, with a protein content of 42%-45%. It is superior to other vegetable c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00A23K10/12A23K20/147
CPCC12P21/00
Inventor 陈建红张济培张伟方
Owner 智农健康产业科技(广东)有限公司
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