Supercharge Your Innovation With Domain-Expert AI Agents!

A kind of preparation method of biological enzyme

A biological enzyme and enzymatic hydrolysis technology, which is applied in the preparation of biological enzymes and in the field of biological enzymes, can solve the problems of low nutrient retention and low added value of products, and achieve the effects of high biological activity, easy operation and wide application

Active Publication Date: 2020-07-31
INST OF PROCESS ENG CHINESE ACAD OF SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems that fruits are difficult to store, the added value of products prepared by traditional processing methods is low, and the retention of nutrients is low, the present invention aims to provide a method for preparing biological enzymes from fruits, which has simple steps and high purity of biological enzymes. high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The raw material is freshly picked Shenzhou peaches, 100kg.

[0051] (1) Pretreatment: Take 100kg of freshly picked Shenzhou peaches as a measurement basis, process the peaches with a peach crushing and beating machine, add 1000kg of water, and use a colloid mill at 0-20°C to grind the peach pulp into fine pieces Pulp, get about 1090kg of peach pulp;

[0052] (2) Enzymolysis: adding 180 g of a mixture of lignin peroxidase and manganese peroxidase to the peach pulp obtained in step (1), adjusting the pH to 4.5-5.0, and stirring at 35° C. for 8 hours;

[0053] (3) Solid-liquid separation: use a filter centrifuge to separate the enzymolyzed mixture obtained in step (2) from solid-liquid, collect the clear liquid, and obtain 1050 kg of permeate;

[0054] (4) Membrane separation 1: using an ultrafiltration membrane with a molecular weight cut-off of 70kDa to perform ultrafiltration on the clear liquid obtained in step (3), collect the permeate, and obtain 850 kg of permeate...

Embodiment 2

[0059] The raw material is peaches that have been stored for 3 days after picking.

[0060] (1) Pretreatment: Take 100kg of peaches that have been placed for 3 days after picking as a measurement basis, use a peach crushing and beating machine to process the peaches, add 1000kg of water, and use a colloid mill to beat the peach pulp at 0-20°C into pulp, obtain peach pulp of about 1085kg;

[0061] (2) Enzymolysis: add 100 g of laccase mixture to the peach pulp obtained in step (1), adjust the pH to 3.5, and stir at 60° C. for 10 h;

[0062] (3) Solid-liquid separation: use a plate-and-frame filter press to separate the enzymolyzed mixture obtained in step (2) from solid-liquid, and collect the clear liquid through tubular centrifugation to obtain 1045 kg of permeate;

[0063] (4) Membrane separation 1: using an ultrafiltration membrane with a molecular weight cut-off of 65kDa to perform ultrafiltration on the clear liquid obtained in step (3), collect the permeate, and obtain ...

Embodiment 3

[0068] The raw material is peaches that have been stored for 3 days after picking.

[0069] (1) Pretreatment: Take 100kg of peaches that have been placed for 3 days after picking as a measurement basis, process the peaches with a peach crushing and beating machine, add 100kg of water, and use a colloid mill at 0°C to beat the peach pulp into a pulp shape, about 190kg of peach pulp was obtained;

[0070] (2) Enzymolysis: add 100 g of lignin-degrading enzyme to the peach pulp obtained in step (1), adjust the pH to 2, and stir at 10°C for 20 hours;

[0071] (3) Solid-liquid separation: use a plate and frame filter press to separate the enzymolyzed mixture obtained in step (2) from solid-liquid, and collect the clear liquid through tubular centrifugation to obtain 165 kg of permeate;

[0072] (4) Membrane separation 1: using an ultrafiltration membrane with a molecular weight cut-off of 50kDa to perform ultrafiltration on the clear liquid obtained in step (3), collect the permeat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a biological enzyme preparation method which includes the steps: (1) pretreatment: beating cleaned fruits into pulp; (2) enzymolysis: adding enzyme into fruit pulp in the step (1), adjusting pH (potential of hydrogen), stirring mixture, performing solid-liquid separation, and collecting liquid supernatant; (3) membrane separation: performing ultra-filtration treatment twice on the liquid supernatant acquired in the step (2) by the aid of an ultra-filtration membrane, collecting permeate liquid in the first ultra-filtration treatment, performing second ultra-filtration treatment on the permeate liquid, and collecting trapped liquid; (4) spray drying: performing spray drying on the trapped liquid acquired in the step (3) to obtain dried frozen products, namely biological enzyme. According to the preparation method, the biological enzyme of various fruits can be extracted, and extracted biological enzyme is high in yield and biological activity. The preparation method can specially extract biological enzyme of peaches, so that extracting effect is best, and the yield and the biological activity of the biological enzyme are the highest.

Description

technical field [0001] The invention belongs to the field of food processing, and relates to a method for preparing biological enzymes, in particular to a method for preparing biological enzymes such as cellulase and pectin enzymolysis by extracting peaches as raw materials step by step. Background technique [0002] Peach (Prunus persica) is the fruit of the genus Prunus in the family Rosaceae. Its shape includes oval, wide oval or oblate, its color changes from light greenish white to orange yellow, and its pulp is white, light greenish white, yellow, orange yellow or The red, juicy flesh of the peach is fragrant, sweet or tart. Peach pulp is rich in glucose, fructose, protein, vitamins, carotene, calcium, phosphorus, iron and other ingredients; peach has a long history of cultivation in my country and is very popular in domestic and foreign markets. [0003] Due to the thin skin of peaches, the picking period is generally in the high temperature season and the harvesting...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N9/26C12N9/18
CPCC12N9/18C12N9/2408C12N9/2437C12N9/2445C12Y301/01011C12Y302/01004C12Y302/01015C12Y302/01021C12Y302/01091
Inventor 张贵锋孔英俊苏志国
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com