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A kind of biological tissue matrix material, preparation method and application thereof

A biological tissue matrix and tissue material technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as tissue regeneration that affects the anti-infection ability of products, product instability, damage to three-dimensional structure, etc., so as to reduce the risk of occurrence , improve efficiency, improve the effect of bonding strength

Active Publication Date: 2018-02-06
BEIJING BIOSIS HEALING BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, Biodesign Surgisis products use common hybrid pigs as animal sources, and individual differences in the genetic background of hybrid animals lead to instability and inconsistency in different batches of products
On the other hand, the molding process used by Biodesign Surgisis products compresses the spatial structure of the SIS matrix material, destroys the natural three-dimensional structure of ECM, and affects the product's ability to resist infection and promote tissue regeneration

Method used

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  • A kind of biological tissue matrix material, preparation method and application thereof
  • A kind of biological tissue matrix material, preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0047] This embodiment relates to a method for preparing an animal-derived implantable biological patch, including the following steps:

[0048] (1) Primary treatment of raw materials:

[0049] Take porcine or bovine small intestinal submucosa tissue material (porcine small intestinal submucosa tissue material is also called porcine small intestinal submucosa SIS) and divide it into a specified size of 8 cm in width and 15 cm in length, remove useless tissues (such as lymphoid tissue), and rinse with tap water for 1- 3 times, and then rinse with purified water until there is no stain on the surface, place the rinsed porcine or bovine small intestinal submucosa tissue material in a water filtration device such as a filter for more than 5 minutes, and drain the water. When taking the small intestine, measure the filtered small intestine with a volume measuring device such as a measuring cylinder.

[0050] (2) Virus inactivation:

[0051] Use peracetic acid-ethanol solution to ...

Embodiment 2

[0059] This embodiment relates to a method for preparing an animal-derived implantable biological patch, including the following steps:

[0060] (1) Primary treatment of raw materials:

[0061] Take pig or bovine small intestinal submucosa tissue material and divide it into specified size 5cm wide and 15cm long, remove useless tissue (such as lymphoid tissue), rinse with purified water until there is no stain on the surface, and wash the pig or bovine small intestinal submucosa material Put it in a water filter device such as a strainer for more than 5 minutes, and drain the water. When taking the small intestine, measure the filtered small intestine with a volume measuring device such as a measuring cylinder.

[0062] (2) Virus inactivation:

[0063] Use peracetic acid-ethanol solution to soak porcine or bovine small intestinal submucosa tissue material for virus inactivation, and this process can be carried out in a stainless steel barrel. The concentration of peracetic a...

Embodiment 3

[0071] The manufacturing method involved in the present invention may also include the following steps:

[0072] (6) Fixed molding:

[0073] Carry out on the mould, said mould includes needle base plate and pressure frame, need to select different molds according to different specifications and sizes, the pig or bovine small intestinal submucosa matrix material prepared in step (5) is spread on the needle base plate , placing the pressure frame on the pig or bovine small intestine submucosa matrix material, the size of the pressure frame can be the final cut size or wider, and relatively fixing the needle base plate and the pressure frame.

[0074] (7) Vacuum freeze drying:

[0075] It is carried out in a vacuum freeze-drying machine. The freeze-drying process of the product needs to be reconfirmed according to different equipment. The mold is laid flat in the vacuum freeze-drying machine, the door of the freeze-drying chamber is closed, the circulation pump is turned on for ...

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Abstract

The invention provides a biological tissue matrix material, and a preparation method and a purpose thereof. The biological tissue matrix material is characterized by comprising an extracellular matrix, wherein the extracellular matrix comprises collagenous fiber, growth factors and fibronectin. The biological tissue matrix material is prepared from small intestinal submucosa matrix materials. The preparation method is characterized by comprising the following steps that decellularization is performed: decellularized liquid contains trypsin and PBS solution, and also contains EDTA, EDTA-2Na or EDTA-4Na; the decellularized liquid is used for treatment in multi-frequency ultrasonic environment for decellularization; the dual-frequency ultrasound at least contains two ultrasonic frequencies with different frequencies. The decellularization process technology is improved; the DNA residual quantity of the obtained product is lower; the immunogenicity is lower; the anti-infection capability is higher; the restoration capability is higher.

Description

technical field [0001] The present invention relates to the technical field of medical biomaterials, in particular to a biological tissue matrix material, a preparation method and its use; and further relates to a biological tissue matrix material after immunogen removal, a specific treatment method and specific applications. Background technique [0002] The repair materials used in medicine can be divided into four categories: the first category is non-absorbable polyester patch, polypropylene patch, expanded polytetrafluoroethylene patch; the second category is absorbable polyglycolic acid, polylactic-glycolic acid; the third category is composite patch; the fourth category is acellular extracellular matrix biological patch (ECM). [0003] It has been reported in the literature that the patch of non-absorbable material: polyester patch, the application of material to repair abdominal incisional hernia, the analysis of complications showed that the recurrence rate was 34%,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
CPCA61L27/3633A61L27/3687A61L27/3691A61L2430/40
Inventor 赵博王洪权赵延瑞李学军张晋辉
Owner BEIJING BIOSIS HEALING BIOLOGICAL TECH
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