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Method for extracting beta-glucan from black highland barley bran

A technology for highland barley bran and glucan is applied in the field of deep processing and comprehensive utilization of grain bran and extraction of beta-glucan, which can solve the problems of complicated purification method and low glucan extraction rate, and achieves the improvement of extraction rate. , the effect of good source of raw materials

Active Publication Date: 2017-08-04
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the above-mentioned technical defects, the main purpose of the present invention is to provide an extraction method that significantly improves the extraction rate of dextran and simplifies the purification steps, so as to solve the problems of low extraction rate of dextran and complicated purification methods

Method used

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  • Method for extracting beta-glucan from black highland barley bran
  • Method for extracting beta-glucan from black highland barley bran
  • Method for extracting beta-glucan from black highland barley bran

Examples

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Effect test

Embodiment 1

[0031] Weigh 50g of black highland barley bran, pass through a 60-mesh sieve after crushing, add 500ml of ethanol (85%), and reflux at 85°C for 2h. In order to achieve the inactivation of endogenous glucanase in raw materials, and play the role of degreasing, decoloring, and removing small molecules. Dry overnight at 40°C to obtain pretreated black highland barley bran powder. The pretreatment preliminarily eliminated some impurities in the subsequent extraction of black barley bran β-glucan, which improved the purity of β-glucan.

[0032] Take by weighing 40g black highland barley bran pretreatment powder, in the black highland barley bran pretreatment powder according to mass volume ratio (w / v) be 1:10 add solvent deionized water, promptly add the deionized water of 400ml , heated in a water bath at 90°C, added high temperature resistant α-amylase and stirred evenly until the iodine-potassium iodide solution did not show blue color. Adjust the pH value to 5 with 1M hydroch...

Embodiment 2

[0034] The method of pretreating black highland barley bran to obtain pretreatment powder is as described in Example 1. Take 30g of pretreated black highland barley bran powder, add 300ml of deionized water as a solvent, heat in a water bath at 90°C, add high temperature resistant α-amylase and stir evenly until the iodine-potassium iodide solution does not show blue. Adjust the pH value to 5 with 1M hydrochloric acid, add xylanase to it, bathe in water at 50-60°C for 30 minutes, and then adjust the pH value to 7 with 1M NaOH. Make up deionized water until the ratio of solid to liquid is 1:10, add NaOH to the mixed solution after removing starch and xylan to a concentration of 1%, extract at room temperature for 2 hours, and keep stirring. After centrifugation at 4000r for 20min, the residue was discarded, and the supernatant was taken for later use. Adjust the pH value of the supernatant to 4.5 with 2M hydrochloric acid, let stand at 4°C for 6 hours, centrifuge at 4000r for ...

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Abstract

The invention discloses a method for extracting beta-glucan from black highland barley bran. The technical process of the method comprises the following steps: preprocessing the black highland barley bran, removing starch and xylan, extracting an alkaline solution, removing proteins, carrying out dialysis on an extracting solution, carrying out concentration and ethanol precipitation, and carrying out redissolving and freeze drying on precipitates, so as to obtain black highland barley bran beta-glucan. By virtue of a combined effect of an enzymatic method and the alkaline solution, the yield of beta-glucan is effectively increased; and by carrying out dialysis and ethanol precipitation, small-molecular impurities and oligosaccharide impurities are effectively removed. According to an extraction process of the method, the yield and purity of beta-glucan are effectively increased, and ethanol can be recycled, so that the extraction cost is lowered; and the adopted raw material is waste, namely the black highland barley bran, produced during the production and processing of black highland barley, so that the economic value of the black highland barley bran is realized.

Description

technical field [0001] The invention relates to the deep processing and comprehensive utilization of grain bran in the field of food processing, in particular to a method for extracting beta-glucan by using black highland barley bran as a raw material. Background technique [0002] Highland barley (Hordeum vulgare Linn.var.nudum Hook.f.) is a cereal crop of the genus Barley in the family Poaceae. It is also called naked barley, barley, and rice barley because of the separation of the inner and outer glumes and the naked grain. It is mainly produced in high-cold and high-altitude areas such as Tibet, Qinghai, Sichuan, and Yunnan in China. Highland barley has a wide range of medicinal and nutritional values. According to the records of "Supplements to Materia Medica", highland barley is used as medicine with a "salty taste and cool nature". The content of β-glucan in highland barley ranks first among wheat crops in the world, with an average content of 6.57%. The content of β...

Claims

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Application Information

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IPC IPC(8): C08B37/02
CPCC08B37/0003C08B37/0024
Inventor 吴艳徐中香艾连中张汇胡浩李季楠
Owner SHANGHAI JIAO TONG UNIV
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