Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biomarker for diagnosis and treatment of abdominal aortic aneurysms

A technology for abdominal aortic aneurysm and drug, which is applied in the field of tumor diagnosis by detecting abnormality of MGAM, and can solve the problem that the pathogenesis of abdominal aortic aneurysm is not completely clear.

Inactive Publication Date: 2017-08-04
QINGDAO MEDINTELL BIOMEDICAL CO LTD
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the specific pathogenesis of abdominal aortic aneurysm is not completely clear. Existing reports have shown that its pathogenesis is closely related to factors such as genetic factors, inflammation, protease degradation, and smooth muscle cell apoptosis. different mutations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biomarker for diagnosis and treatment of abdominal aortic aneurysms
  • Biomarker for diagnosis and treatment of abdominal aortic aneurysms
  • Biomarker for diagnosis and treatment of abdominal aortic aneurysms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Gene Chip Screening for Differentially Expressed Genes

[0060] 1. Tissue acquisition and processing: Collect 2 live specimens of the middle layer of the AAA artery wall and 2 copies of the normal infrarenal aortic control tissue. Under sterile and RNase-free conditions, cut the aortic wall at about 4 cm below the flat renal artery. Rinse with sterilized normal saline to remove blood stains, remove arterial intima and intima from the collected tissues, and quickly (<5 min) freeze them in liquid nitrogen for later use.

[0061] 2. RNA extraction and cDNA synthesis

[0062] The aneurysm tissues preserved in liquid nitrogen were placed in a ceramic mortar under the low temperature environment of liquid nitrogen and crushed into powder, added Trizol reagent to homogenate, centrifuged, and the supernatant was extracted twice with 1:1 acidic phenol-chloroform After extraction with sodium acetate and 5:1 acidic phenol-chloroform, an equal volume of isopropanol preci...

Embodiment 2

[0071] Example 2 Verification of Differentially Expressed Genes in Large Samples

[0072] The expression in abdominal aortic aneurysm tissue was selected to select gene chip prompting differentially expressed MGAM as the research target for reverse validation.

[0073] 1. Tissue acquisition and processing: According to the method in Example 1, 30 live specimens of the middle layer of the AAA artery wall and 40 normal infrarenal aortic control tissues were collected.

[0074] 2. RNA extraction

[0075] RNA extraction was carried out according to the method of Example 1.

[0076] 3. Reverse transcription

[0077] 1 μg of total RNA was reverse-transcribed to synthesize cDNA using reverse transcription buffer. Use 25μl reaction system, take 1μg total RNA for each sample as template RNA, add the following components to the PCR tube respectively: DEPC water, 5× reverse transcription buffer, 10mmol / L dNTP, 0.1mmol / l DTT, 30μmmol / l Oligo dT, 200 U / μl M-MLV, template RNA. Incubate...

Embodiment 3

[0088] Embodiment 3 interferes with MGAM gene expression

[0089] 1. Design and synthesis of interference RNA

[0090] According to the MGAM gene sequence, siRNA was designed and synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd. Shanghai Gemma Pharmaceutical Technology Co., Ltd. also provides a negative control siRNA (siRNA-NC) that has no sequence homology with the MGAM gene.

[0091] siRNA-MGAM:

[0092] The sense strand is 5'-AGAACAUUGUCAACAUUGCUU-3' (SEQ ID NO.5);

[0093] The antisense strand is 5'-GCAAUGUUGACAAUGUUCUUC-3' (SEQ ID NO.6),

[0094] 2. Culture of human aortic smooth muscle cells

[0095] T / G HA-VSMC cells (referred to as HVSMC cells) were treated with DMEM high-glucose medium containing 10% fetal bovine serum (FBS) plus penicillin 100 units / ml and streptomycin 100 μg / ml, placed at 37°C, 5% CO 2 Cultured in an incubator, the culture medium was changed every 24 hours, and the cells were passaged once every 48 hours. Cells in the logarithm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a MGAM gene as a biomarker for diagnosis of abdominal aortic aneurysms. Experimental results in the invention prove that the expression of the MGAM gene in the tissue of abdominal aortic aneurysms substantially increases compared with the expression of the MGAM gene in normal control tissue; and interference experiment results prove that the MGAM gene can influence the apoptosis of human aortic smooth muscle cells. According to the research results of the invention, a drug capable of inhibiting the expression of the MGAM gene can be developed so as to prevent and treat the abdominal aortic aneurysms clinically.

Description

technical field [0001] The invention relates to the field of tumor diagnosis and treatment, more specifically, the invention relates to a tumor diagnosis method by means of detecting MGAM abnormality; and a tumor therapeutic agent for activating MGAM gene or protein. Background technique [0002] Abdominal aortic aneurysm (AAA) refers to a disease characterized by localized expansion and bulging of the abdominal aorta due to lesion or loss of the abdominal aortic wall, with a pulsating mass as the main symptom. AAA is usually defined as the continuous expansion of the three-layer structure of the abdominal aortic arterial wall to more than 1.5 times the diameter of the abdominal aorta at the renal artery. It is a common arterial degenerative disease with a high mortality rate. At present, the specific pathogenesis of abdominal aortic aneurysm is not completely clear. Existing reports have shown that its pathogenesis is closely related to factors such as genetic factors, infl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68G01N33/574A61K45/00A61P35/00
CPCA61K45/00C12Q1/6886C12Q2600/158G01N33/57407G01N33/57484G01N2333/928
Inventor 李曙光孙锦云
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com