Method for extracting and separating succinic acid from fermentation liquid by using macroporous adsorption resin

Abstract

The invention relates to a method for extracting and separating succinic acid from fermentation liquid by using macroporous adsorption resin. The method includes the following steps that 1, succinic acid fermentation liquid is pretreated to remove solid particles and macromolecule impurities in the fermentation liquid and obtain pretreatment liquid; 2, the pretreatment liquid is regulated to be acidic and then delivered to an adsorption column loaded with the polar macroporous adsorption resin, and the polar macroporous adsorption resin is used for adsorption of succinic acid in the pretreatment liquid; 3, an eluting agent is introduced in the adsorption column for eluting to obtain succinic acid eluent; 4, the succinic acid eluent is regulated to be acidic, then concentration, crystallization, filtration and drying are conducted in sequence, and succinic acid is obtained. Compared with the prior art, the polar macroporous adsorption resin is used for extracting and separating high-impurity succinic acid from the succinic acid fermentation liquid, the process is mild in condition, energy consumption is low, operation is simple and convenient, few solvent is consumed, the process is short, and large-scale commercial production is easy to achieve.

Description

technical field [0001] The invention belongs to the technical field of biological separation engineering, and relates to a method for extracting and separating succinic acid from fermentation liquid by using a macroporous adsorption resin. Background technique [0002] Succinic acid, also known as succinic acid, is an important platform compound that can be derived from various bulk chemicals such as butanediol, tetrahydrofuran and γ-butyrolactone. The preparation method of succinic acid includes chemical synthesis method and microbial fermentation method. Among them, the chemical synthesis method needs to consume a large amount of petrochemical raw materials, and the energy consumption and cost are high; the microbial fermentation method uses biological resources with low cost and greenhouse gas carbon dioxide as raw materials. , under suitable conditions, succinic acid is produced by bacteria or other microorganisms through a specific metabolic pathway, which has many adva...

AI Technical Summary

Problems solved by technology

In the technical scheme announced by the above-mentioned patent, D3520 type, H103 type non-polar macroporous adsorption resin or D301 type weakly basic anion exchange resin are used as auxiliary separation means, and the pigment in the fermentation broth is subjected to one-time separation at a higher temperature. Adsorption and removal improves the purity of the product to a certain extent, but due to the complex types of impurities in the fermentation broth, in addition to pigments, it also contains a large amount of protein, inorganic salts, sugars and various microbial metabolites, etc., it is difficult to use a single type At the same time, the resin completely adsorbs and removes all the impurities therein, the impurity removal effect is often poor, and the product quality is poor, and this process has no concentration and enrichment effect on the active ingredient (succinic acid) in the fermentation broth, that is, decolorization The concentration of succinic acid in the fermentation broth before and after is basically unchanged. Therefore, if the concentration of succinic acid in the fermentation broth itself is low, the energy consumption in the subsequent evaporation and concentration process will be high, which is not conducive to large-scale commercial production.