Lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and strains, applied in the direction of Lactobacillus, application, bacteria, etc., can solve the problems of low acid production, slow growth of lactic acid bacteria, and low quality of feed mulberry silage, achieve low cost, accelerate fermentation process, and overcome fermentation quality poor effect

Inactive Publication Date: 2017-08-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems of slow growth of lactic acid bacteria and low acid production in the silage preparation process in the prior art, which lead to low quality of feed mulberry silage, and propose a method that can quickly reduce the pH value, accelerate the fermentation process, and effectively improve the quality of feed mulberry silage. Silage-quality Lactobacillus plantarum

Method used

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  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Isolation and strain identification of plant Lactobacillus (Lactobacillus plantarum) ZS1

[0037] 1. Isolation of strains

[0038] Separation of Lactobacillus plantarum by the dilution plate method: Weigh 10 g of the feed Kuwahara under sterile conditions, place it in a conical flask filled with 90 mL of sterile distilled water, shake well, and obtain a 10-fold diluted sample suspension, and then press 10-fold The dilution method can be directly serial dilution. Select an appropriate dilution, take 0.1 mL and spread it on the MRS medium plate, incubate in a 30°C incubator for 48 hours, and count the plates. According to the characteristics of colony size, shape and color, typical colonies were picked for Gram staining microscopy and catalase test. All strains that are Gram-positive and catalase-negative can be preliminarily identified as Lactobacillus, and they are streaked and purified twice on MRS medium, inoculated into MRS solid slant medium, and stored...

Embodiment 2

[0067] Embodiment 2: the preparation of fodder mulberry silage

[0068] The preparation of feed mulberry silage comprises the following steps:

[0069] (1) Cut the slightly air-dried feed mulberry to about 2cm, and mix evenly;

[0070] (2) Add plant Lactobacillus (Lactobacillus plantarum) ZS1 to step (1) feed mulberry, add about 1.0×10 plant Lactobacillus (Lactobacillus plantarum) ZS1 per kilogram of feed mulberry 6 , and with no addition (CK) and GFG (commercial bacterial agent) as controls, each treatment was repeated 3 times;

[0071] (3) Put the feed mulberry in step (2) into polyethylene silage bags of 30cm×20cm, 100g in each bag, pump air with a vacuum sealing machine, seal, and store at room temperature for 60 days.

[0072] 1. Determination of nutritional quality of silage

[0073] The routine nutrient composition determination method of feed mulberry silage is as follows:

[0074] Determination of dry matter (DM): Utilize blast drying oven, feed mulberry silage at...

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Abstract

The invention discloses lactobacillus plantarum and application thereof, and belongs to the field of technologies for processing silage. A preservation number of the Lactobacillus plantarum ZS1 is CGMCC No.13460. The lactobacillus plantarum can be used for preparing silage and particularly can be used for preparing forage mulberry silage. The lactobacillus plantarum ZS1 and the application have the advantages that a pH (potential of hydrogen) value of the silage can be quickly lowered by the lactobacillus plantarum ZS1, fermentation procedures can be accelerated, and the silage speeds can be increased; the quality of the forage mulberry silage can be effectively improved, the quantities of contaminating microorganisms in the forage mulberry silage can be reduced, nutrition in forage mulberry can be effectively preserved, the problem of poor feed fermentation quality can be solved, and the lactobacillus plantarum can be widely used; silage effects of additives prepared from the lactobacillus plantarum ZS1 are superior to silage effects of commercially available lactic acid bacteria additives, and the additives are low in cost and easy to use and are safe.

Description

technical field [0001] The invention belongs to the technical field of silage processing, and in particular relates to plant lactobacillus and application thereof. Background technique [0002] With the increase of population and the development of industry, my country's animal husbandry has developed rapidly, and the problem of competition between humans and animals for food has become increasingly acute, which directly affects the development of animal husbandry. Therefore, the scientific and rational development of forage plant resources is the material basis for the development of animal husbandry and an important way to solve the shortage of pasture resources. Chinese researchers have bred the latest stress-resistant strain - feed mulberry, because of its rich nutrition, good palatability, easy digestion, high protein and calcium content, potential ecological value and feed value, the development prospect is very broad. Silage is yellow-green in color, sour in smell, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18A23K10/12C12R1/25
CPCC12R2001/25C12N1/205A23V2400/169
Inventor 杨富裕张颖超李东霞王学凯玉柱
Owner CHINA AGRI UNIV
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