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Cysteine determination ratio fluorescence sensor as well as preparation method and application thereof

A ratiometric fluorescence, cysteine ​​technology, applied in the field of organic-inorganic composite cysteine ​​ratio fluorescence sensor, can solve the problems of measurement result error, fluorescence signal data distortion, etc., achieve low detection limit, long storage time, The effect of wide detection range

Active Publication Date: 2017-08-15
BEIJING UNIV OF CHEM TECH
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Problems solved by technology

Therefore, when the fluorescence intensity is measured at a single wavelength, the fluorescence signal data is often distorted in practical applications, resulting in errors in the measurement results

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  • Cysteine determination ratio fluorescence sensor as well as preparation method and application thereof
  • Cysteine determination ratio fluorescence sensor as well as preparation method and application thereof
  • Cysteine determination ratio fluorescence sensor as well as preparation method and application thereof

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Embodiment 1

[0035] 1. Preparation of hydrotalcite precursor: 0.008mol Mg(NO 3 ) 2 ·6H 2 O, 0.04mol Al(NO 3 ) 3 9H 2 O and 0.048mol urea were dissolved in 280ml of deionized water, placed in four 90ml polytetrafluoroethylene-lined pressure reaction vessels, reacted at 110°C for 24 hours, and were centrifugally washed with deionized water until the pH value was about Drying in the air at 7 and 60°C to obtain carbonate-type anion-intercalated hydrotalcite microcrystals. Take 0.3 g of the above-mentioned carbonate anionic hydrotalcite and solid NaNO 3 63.75g dissolved in 300mL to remove CO 2 After being uniformly dispersed in deionized water, add 0.08mL of concentrated nitric acid to acidify and stir at 25°C under nitrogen atmosphere to carry out ion exchange reaction. After 48 hours remove CO with 2 The deionized hot water was centrifuged and washed to a pH of about 7, and dried at 60° C. for 12 hours to obtain a hydrotalcite intercalated with magnesium aluminum nitrate anions.

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Abstract

The invention provides a cysteine determination ratio fluorescence sensor as well as a preparation method and application thereof. According to the invention, a calcein@nuclear fast red / hydrotalcite composite thin film is obtained by virtue of a layer-by-layer self-assembly method, the fluorescence sensor applies a ratio fluorescence method, two fluorescence intensity ratios of different wavelengths are utilized for calibration, a background signal which is not interfered by an environment is provided, high optical stability of a fluorescence probe is combined with the advantages of a ratio fluorescence probe, background interference can be effectively avoided due to no influence of light source intensity and instrumental sensitivity, and the sensitivity and selectivity of the ratio fluorescence probe are greatly improved; the preparation method is simple and practical, the cost is low, and reuse can be realized; selectivity on detection of cysteine is good, a linear relationship is shown within a certain range, and the characteristics of high sensitivity, low detection limit, high efficiency and speediness are realized; and the thin film also maintains the characteristics of hydrotalcite, namely no toxicity, stability, good biocompatibility and difficulty in ageing.

Description

technical field [0001] The invention belongs to the technical field of fluorescence sensor preparation, and provides a cysteine ​​measurement ratio fluorescence sensor, a preparation method and application, in particular, an organic-inorganic composite cysteine ​​ratio fluorescence sensor is obtained by using a supramolecular self-assembly method. Background technique [0002] In the field of biochemistry, cysteine ​​is a common non-essential amino acid that contains the only thiol group in human proteins. Changes in the content of cysteine ​​in the human body often cause the occurrence of some diseases. For example, when the concentration of cysteine ​​exceeds the normal level, it will lead to atherosclerosis, coronary heart disease and cerebral infarction. Cysteine ​​is also involved in metabolism, protein synthesis, detoxification, defense and other vital activities in organisms. Therefore, efficient and rapid detection of cysteine ​​has become one of the hot topics in ...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6486G01N2021/6432
Inventor 靳兰李明哲付艳艳卫敏
Owner BEIJING UNIV OF CHEM TECH
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