Method for utilizing various vinasse for preparing albumen powder and polypeptide powder
A technology of polypeptide powder and protein powder, which is applied in protein food processing, protein food ingredients, protein-containing food ingredients, etc., can solve the problems of not specifying how to deal with protein residue, staying in the laboratory research stage, and difficult implementation. Achieve the effects of reducing uncontrollable risks, improving refining efficiency and product purity, and increasing added value
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Embodiment 1
[0031] Follow the steps below to prepare distiller's grain polypeptide and protein powder, the steps are as follows:
[0032] (1) Pretreatment of distiller's grains: select yellow distiller's grains that have no mildew, sour smell and obvious qualitative change, and have been processed by fermentation process, and wash to remove residual alcohol;
[0033] (2) Pulverization: After the pretreated solid slag is coarsely pulverized, finely pulverized, and sieved, the slurry and slag are separated; during sieving, the mesh number of the screen is 60 mesh, and the fineness of the material after fine pulverization is 300 mesh.
[0034] (3) One-time enzymatic hydrolysis: adjust the slurry concentration to 10%, pH to 5.5, material temperature to 90°C, add high-temperature amylase, the addition amount is 0.2‰g / g dry matter, stir for 0.5h, and 85°C after the reaction Inactivate the enzyme for 10 minutes;
[0035] (4) Microbial fermentation: adding yeast dry powder to the reaction soluti...
Embodiment 2
[0042] Follow the steps below to prepare distiller's grain polypeptide and protein powder, the steps are as follows:
[0043] (1) Distiller's grains pretreatment: select distiller's grains that have no mildew, sour smell and obvious qualitative change, and have been processed by fermentation process, and wash to remove residual alcohol;
[0044] (2) Pulverization: After the pretreated solid slag is coarsely pulverized, finely pulverized, and sieved, the slurry and slag are separated; during sieving, the mesh number of the screen is 60 mesh, and the fineness of the material after fine pulverization is 300 mesh.
[0045] (3) One-time enzymatic hydrolysis: adjust the slurry concentration to 15%, pH to 6.0, and material temperature to 95°C, add high-temperature amylase at an amount of 0.4‰g / g dry matter, stir for 1 hour, and extinguish at 85°C after the reaction Enzyme 10min;
[0046] (4) Microbial fermentation: add dry yeast powder to the reaction solution obtained in step (3), ...
Embodiment 3
[0053] Follow the steps below to prepare distiller's grain polypeptide and protein powder, the steps are as follows:
[0054] (1) Pretreatment of distiller's grains: select brewer's grains that have no mildew, sour smell and obvious qualitative change, and have been treated by fermentation process, and wash to remove residual alcohol;
[0055] (2) Pulverization: After the pretreated solid slag is coarsely crushed, finely crushed, and sieved, the slurry and slag are separated; during sieving, the mesh number of the screen is 80 mesh, and the fineness of the material after fine crushing is 300 mesh.
[0056] (3) One-time enzymatic hydrolysis: adjust the slurry concentration to 20%, pH to 6.5, and material temperature to 100°C, add high-temperature amylase at an amount of 0.5‰g / g dry matter, stir for 2 hours, and extinguish at 85°C after the reaction Enzyme 10min;
[0057] (4) Microbial fermentation: add dry yeast powder to the reaction solution obtained in step (3), the yeast u...
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