Silk fibroin sponge and preparation method thereof

A technology of silk protein and silk protein solution, which is applied in the fields of medical formula, medical science, prosthesis, etc., and can solve the problems of silk protein precipitation and uneven porous sponge

Active Publication Date: 2017-08-22
丝纳特(苏州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above technology requires that the content of high crystalline silk protein nanofibers should not be lower than 7.5%, otherwise the prepared porous sponge will be partia...

Method used

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  • Silk fibroin sponge and preparation method thereof
  • Silk fibroin sponge and preparation method thereof
  • Silk fibroin sponge and preparation method thereof

Examples

Experimental program
Comparison scheme
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preparation example Construction

[0030] Thus, the application provides the preparation method of the above-mentioned silk protein sponge, comprising the following steps:

[0031] A), the silk protein aqueous solution is sealed and cultivated and then mixed with the highly crystalline silk protein nanofiber solution to obtain a mixed solution;

[0032] B), the mixed solution is frozen and then freeze-dried to obtain silk protein sponge.

[0033] In this application, a small amount of highly crystalline silk protein nanofiber is used as an inducer, mixed with a treated silk protein solution, and then sequentially frozen and freeze-dried to obtain a silk protein sponge.

[0034] In the process of preparing the silk protein sponge, the applicant first sealed and cultivated the silk protein aqueous solution; after the sealed cultivation, the conformation of the random silk protein gradually changed to an intermediate state. The silk protein is a silk protein well known to those skilled in the art, and there is no...

Embodiment 1

[0047] (1) Slowly concentrate the ordinary silk protein solution in the range of 40-60°C to obtain a solution with a concentration of about 10%;

[0048] (2) Transfer the solution to normal temperature and continue to concentrate to about 20%, during which the silk protein transforms into metastable nanoparticles;

[0049] (3) The concentrated solution is diluted with water to 0.5%-2%, placed at 60° C. and incubated in a sealed manner until obtaining highly crystalline silk protein nanofibers.

[0050] figure 1 and figure 2 Respectively, the macroscopic and microscopic diagrams of the highly crystalline silk protein nanofibers prepared in this example, image 3 Infrared spectrum of the nanofibers prepared for this example; as can be seen from the figure, the highly crystalline silk protein nanofibers prepared in this implementation are in a solution state, the fiber diameter is between 10 and 20 nm, and the length is between 200 nm and 3 μm. The infrared spectrum shows that...

Embodiment 2

[0053] (1) After a certain concentration of common silk protein solution was sealed and incubated at 40°C for 48 hours, it was mixed with the high crystallization silk protein nanofiber solution. The mass ratio of high crystallization silk protein nanofiber to common silk protein was 1:40, and two kinds of A mixed aqueous solution of silk protein and injected into the mould;

[0054] (2) freezing the silk protein nanofiber solution injected into the mold at -7°C for 24 hours to form ice;

[0055] (3) Place the frozen ice-like substance in a lyophilizer with a cold trap temperature of -63° C., and freeze-dry for 48 hours to obtain a water-insoluble silk protein sponge.

[0056] Figure 4 and Figure 5 The high-power and low-power electron micrographs of the porous silk protein sponge material prepared in this example are respectively. It can be seen from the figure that the porous silk protein sponge prepared in this example has a pore diameter between 100 and 200 μm, and the...

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Abstract

The invention discloses silk fibroin sponge. The content of beta-sheet is lower than 30 percent by weight; the silk fibroin sponge is insoluble in water. The invention also provides a preparation method of the silk fibroin sponge, comprising the following steps: A) sealing and cultivating a silk fibroin water solution, and then mixing with a high-crystallized silk fibroin nanofiber solution to obtain a mixed solution; B) freezing the mixed solution, and then freeze-drying to obtain the silk fibroin sponge. A little amount of high-crystallized silk fibroin nanofiber is taken as an inducer and is mixed with the treated silk fibroin water solution, and a silk fibroin porous sponge material insoluble to water is directly prepared from the water solution by freezing and freeze-drying in sequence. According to the method, high-crystallized silk fibroin nanofiber induction, temperature treatment and silk fibroin assembly rate regulation and control in the freezing process are combined, the porous sponge insoluble to the water is obtained without adding any other solvent or treatment process, the technology is simple and environment-friendly, and the biocompatibility is excellent.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a silk protein sponge and a preparation method thereof. Background technique [0002] The excellent biocompatibility, mechanical properties, degradability, easy shaping, and ability to be processed in aqueous solution have made silk protein a versatile biomaterial with potential applications. Silk protein sponge with porous structure has been widely used in the repair of skin, bone, nerve, cartilage and other tissues, and achieved good results. How to use mild, stable and simple methods to prepare silk protein porous sponges, and to regulate the mechanical and biological properties of porous sponges has become an important development direction of silk protein biomaterials. [0003] At present, researchers have developed a variety of methods for preparing silk protein sponges, mainly including salting-out method and freeze-drying method; the main conformation of por...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/56A61L27/58
CPCA61L27/227A61L27/56A61L27/58A61L2400/12A61L2430/34
Inventor 祝文莉吕强
Owner 丝纳特(苏州)生物科技有限公司
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