Method of quantitatively detecting organophosphorus pesticide by constructing colorimetric sensor based on gold nanorod etching

An organophosphorus pesticide and colorimetric sensor technology, applied in the field of biosensing, can solve problems such as increase, and achieve the effects of low cost, rich variety and convenient operation.

Inactive Publication Date: 2017-08-22
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The increased variety of colors in colorimetric sensors can significantly imp

Method used

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  • Method of quantitatively detecting organophosphorus pesticide by constructing colorimetric sensor based on gold nanorod etching
  • Method of quantitatively detecting organophosphorus pesticide by constructing colorimetric sensor based on gold nanorod etching
  • Method of quantitatively detecting organophosphorus pesticide by constructing colorimetric sensor based on gold nanorod etching

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Embodiment 1

[0040] A method for quantitatively detecting organophosphorus pesticides by constructing a colorimetric sensor based on gold nanorod etching, the detection steps are:

[0041] Steps for one-pot synthesis of gold nanorods: first, soak glass instruments such as beakers used in the experiment with aqua regia (hydrochloric acid: nitric acid = 3:1) for 24 hours. Then, to prepare the gold nanorod growth solution, 1520 μL of chloroauric acid (10 mmol / L), 222 μL of silver nitrate (20 mmol / L) and 1 mL of hydroquinone (5.26 mmol / L) were sequentially added to 34.612 mL of CTAB, and gently Shake and stand for 5 minutes, finally add 646 μL of sodium borohydride (1 mmol / L) and let stand for 12 hours. All experimental operations are carried out at 30°C. Then the resulting solution was centrifuged at 15,000×g for 20 minutes, redissolved in 40 mL of ultrapure water after centrifugation twice, and stored in a brown bottle at room temperature.

[0042] Acetylcholinesterase detection steps: Take...

Embodiment 2

[0047] A method for quantitatively detecting organophosphorus pesticides by constructing a colorimetric sensor based on gold nanorod etching, the detection steps are:

[0048] Steps for one-pot synthesis of gold nanorods: first, soak glass instruments such as beakers used in the experiment with aqua regia (hydrochloric acid: nitric acid = 3:1) for 24 hours. Then, to prepare the gold nanorod growth solution, 1520 μL of chloroauric acid (10 mmol / L), 222 μL of silver nitrate (20 mmol / L) and 1 mL of hydroquinone (5.26 mmol / L) were sequentially added to 34.612 mL of CTAB, and gently Shake and stand for 5 minutes, finally add 646 μL of sodium borohydride (1 mmol / L) and let stand for 12 hours. All experimental operations are carried out at 30°C. Then the obtained solution was centrifuged at 15000×g for 20 minutes, redissolved in ultrapure water after centrifugation twice, and stored in a brown bottle at room temperature.

[0049] Acetylcholinesterase detection steps: Take an EP tube...

Embodiment 3

[0054] A method for quantitatively detecting organophosphorus pesticides by constructing a colorimetric sensor based on gold nanorod etching, the detection steps are:

[0055] Steps for one-pot synthesis of gold nanorods: first, soak glass instruments such as beakers used in the experiment with aqua regia (hydrochloric acid: nitric acid = 3:1) for 24 hours. Then, to prepare the gold nanorod growth solution, 1520 μL of chloroauric acid (10 mmol / L), 222 μL of silver nitrate (20 mmol / L) and 1 mL of hydroquinone (5.26 mmol / L) were sequentially added to 34.612 mL of CTAB, and gently Shake and stand for 5 minutes, finally add 646 μL of sodium borohydride (1 mmol / L) and let stand for 12 hours. All experimental operations are carried out at 30°C. Then the obtained solution was centrifuged at 15000×g for 20 minutes, redissolved in ultrapure water after centrifugation twice, and stored in a brown bottle at room temperature.

[0056] Acetylcholinesterase detection steps: Take an EP tube...

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Abstract

The invention belongs to the technical field of biosensing and relates to a method of detecting an organophosphorus pesticide based on a gold nanorod and a colorimetric array. The method comprises the following steps: catalytically chloridizing acetylcholine by adopting a double enzyme system to produce hydrogen peroxide, etching the gold nanorod by the hydrogen peroxide under the action of a catalysis condition, and observing a color after reaction, wherein the double enzyme system is a system containing acetylcholinesterase and choline oxidase at the same time, and the step of etching the gold nanorod by the hydrogen peroxide under the action of the catalysis condition is a step of etching the gold nanorod by the hydrogen peroxide in an Fe<2+> containing acid solution. The method can simply, rapidly, accurately, quantitatively and visually detect the organophosphorus pesticide.

Description

technical field [0001] The invention belongs to the technical field of biosensing, and relates to a method for detecting organophosphorus pesticides based on gold nanorods and a colorimetric array, in particular to a method for quantitatively detecting organophosphorus pesticides by constructing a colorimetric sensor based on gold nanorod etching. Background technique [0002] In recent years, food safety and environmental problems caused by the abuse of organophosphorus pesticides in my country have attracted more and more attention. Organophosphorus pesticides are widely used in the control of crop diseases and insect pests due to their advantages of various varieties, high insecticidal efficiency, easy decomposition, and little damage to plants. However, most organophosphorus pesticide species are highly toxic. After they enter the human body through breathing, skin and gastrointestinal absorption, they can inhibit the activity of cholinesterase in the body, because they...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 卫敏吕冰婧卫伟
Owner HENAN UNIVERSITY OF TECHNOLOGY
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