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Preparation method of amygdalin

A kind of technology of amygdalin and bitter almond, which is applied in the field of preparation of amygdalin, can solve the problems of low sample yield, high pollution, low production efficiency, etc., achieve large single-time preparation, retain biological activity, and avoid sample loss Effect

Active Publication Date: 2017-09-01
UNO SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low yield of samples prepared by macroporous resin column chromatography, normal phase column chromatography and reversed phase column chromatography, etc., the preparation volume is small, the pollution is large, the time is long, and the cost is high, so it is impossible to carry out large-scale preparation. Moreover, due to the use of a large amount of adsorption fillers in the preparation process, the original biological activity of the sample is greatly reduced, the activity of the prepared material is reduced, and the production efficiency is low, so it is not suitable for popularization and use.

Method used

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  • Preparation method of amygdalin
  • Preparation method of amygdalin
  • Preparation method of amygdalin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Prepare amygdalin as follows:

[0035] S1. Take 1.1 kilograms of bitter almond medicinal materials as raw materials, smash through a 60-mesh sieve, inactivate enzymes at 60°C in an oven for 60 minutes, add petroleum ether in a ratio of 1:3, reflux in a water bath at 70°C for 50 minutes, degreasing, pumping The filter residue was collected by filtration, and alcohol was added to dissolve the filter residue at a ratio of material-to-liquid ratio of 1:10. Ultrasonic extraction was performed at a power of 60w and a water bath of 70 ° C for 50 minutes. Filtered, and the filtrate was placed separately. Concentrated to obtain 204.9 g of amygdalin extract.

[0036] S2. Separation by normal phase column chromatography: take 27 g of the extract, use ethyl acetate-methanol with a volume ratio of 85:15 as the elution solvent and silica gel as the filler to carry out normal phase column chromatography to separate the extract of the amygdalin , before separation, the filler silica g...

Embodiment 2

[0040] Prepare amygdalin as follows:

[0041] S1. Extract preparation: directly take the extract prepared by the method described in Step 1 of Example 1.

[0042] S2. Separation by normal phase column chromatography: the same as in Example 1.

[0043] S3. Purification by high-speed countercurrent chromatography: similar to Example 1, the difference is that the volume ratio of the solvent system ethyl acetate-n-butanol-water is 0.5:5:4.

Embodiment 3

[0045] Prepare amygdalin as follows:

[0046] S1. Extract preparation: directly take the extract prepared by the method described in Step 1 of Example 1.

[0047] S2. Separation by normal phase column chromatography: the same as in Example 1.

[0048] S3. Purification by high-speed countercurrent chromatography: similar to Example 1, the difference is that the volume ratio of the solvent system ethyl acetate-n-butanol-water is 1.5:1:1.

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Abstract

The invention discloses a method for preparing amygdalin, comprising the following steps: S1, preparation of an extract: fetching a bitter apricot kernel medicinal material as a raw material, crushing and sieving, carrying out enzyme deactivation, degreasing, filtering and fetching filter residue, dissolving with alcohol, carrying out ultrasonic extraction, filtering, and concentrating a filtrate to obtain an extract mainly containing amygdalin; S2, normal phase column chromatographic separation: using ethyl acetate-methanol as an elution solvent and using silica gel as a filling material and carrying out normal phase column chromatographic separation on the extract of amygdalin, collecting the elution solvent containing amygdalin, and carrying out reduced pressure distillation to obtain an amygdalin crude product; and S3, high-speed counter-current chromatographic purification: using ethyl acetate-n butanol-water as a solvent system, and carrying out high-speed counter-current chromatographic purification on amygdalin. By the above preparation method, purity of the prepared amygdalin is high, and one-time preparation amount is large. There is no influence on activity of the sample, and there is no additional pollution. The product can be applied to medicines, cosmetic, health products and the like.

Description

technical field [0001] The invention relates to the technical field of extraction, separation and purification of effective components of traditional Chinese medicines, and in particular, the invention relates to a preparation method of amygdalin. Background technique [0002] Amygdalin is a main component of bitter almond, which has the functions of antitussive and relieving asthma, anti-tumor, lowering blood pressure and anticoagulant. Qi stagnation, intestinal dryness, constipation, edema and fullness have good clinical treatment effect. At present, most of the methods used for separation of amygdalin are macroporous resin column chromatography, combined normal-phase column chromatography and reversed-phase column chromatography, and crystallization. Due to the low yield, small preparation, large pollution, long time and high cost of preparing samples by macroporous resin column chromatography, normal-phase column chromatography and reversed-phase column chromatography, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08
CPCC07H1/08C07H15/18
Inventor 黄启吟连林生曾仑
Owner UNO SCI CO LTD
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