Method for replacing process water and nitrogen source in bio-based chemical production with biogas slurry
A technology for bio-based chemicals and production processes, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of high cost of water and nitrogen sources, and achieve low production costs, high output value, and low prices Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] This example describes the analysis of corn stover components.
[0032] The raw materials used in this technological method include corn stover. After the sample is naturally dried, it is pulverized and passed through a 20-80 mesh sieve and stored at -20 ° C for subsequent use. Component analysis samples were prepared according to NREL-LAP001, and the contents of cellulose, hemicellulose, and lignin components of the raw materials were determined according to standard methods. The results of component determination are shown in Table 1.
[0033] Table 1 Analysis of components of corn stover
[0034]
Embodiment 2
[0036] This example describes the NaOH pretreatment of corn stover.
[0037] Take the corn stalks dried at 105°C overnight and add 5 g of corn stalks to 11 groups of hydrothermal reactors at a solid-to-liquid ratio of 1:10, and add 0.67 g of NaOH solid (1.34%, w / v), add biogas slurry with concentrations of 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% respectively, with a total volume of 50 mL (such as shown in Table 2). Stir well, place the hydrothermal reaction kettle in an oven, treat at 140 °C for 6 h, transfer to a centrifuge tube after treatment, centrifuge at 4,500 rpm for 5 min for solid-liquid separation, and use 3,5-dinitrosalicylic The concentration of reducing sugar in the supernatant was determined by acid (DNS) colorimetric method, and the concentration of corn stover treated with biogas slurry was determined with the yield of reducing sugar as the index.
[0038] Table 2 Effects of different biogas slurry concentrations on the yield of reducing sug...
Embodiment 3
[0042] This example describes the enzymatic hydrolysis (continuous enzymatic hydrolysis) of pretreated corn stover.
[0043] Take 0.3 g of corn stalks dried at 105 °C under the optimal pretreatment conditions into a 50 ml centrifuge tube, add 30 ml of pH 4.8 0.05M sodium citrate buffer, 350 μL of cellulase, and at 50 °C, 120 rpm. Vibration treatment for 72 h. After the treatment, the centrifuge tube was placed in a 100°C water bath to inactivate the enzyme activity to terminate the reaction. The centrifuge tube was centrifuged at 4,500 rpm for 3 min. The concentration of reducing sugar in the supernatant was determined by DNS method. (HPLC) to determine the sugar components in the enzymatic hydrolyzate, and the results of the component content determination are shown in Table 3.
[0044] Table 3 The effect of cellulase on the yield of reducing sugar by hydrolysis of corn stover pretreated with biogas slurry
[0045]
[0046] The effect of cellulase on the yield of reducing...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More