Preparation method and application of pholiota nameko active peptide
A technology of active peptides and mushrooms, applied in the field of preparation of active peptides of mushrooms, can solve the problems of not being specific to mushrooms, not involving mushroom proteins, and needing to be improved, etc., and achieving low cost, good product safety, and technology. simple effect
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Embodiment 1
[0019] Example 1 Preparation of Pleurotus ostreatus Active Peptide
[0020] (1) Pretreatment: crush the dried fruiting bodies of Pleurotus ostreatus, pass through a 60-mesh sieve, mix with distilled water at a material-to-liquid ratio of 1:20 (W / V), and make pulp, that is, Pleurotus chinensis slurry;
[0021] (2) Extraction: put the slippery mushroom slurry in an ultrasonic extractor, control the ultrasonic frequency at 500W, add laccase (10 4 U / g), adjust the pH value to 5.0, and treat at 40°C for 2 hours; then treat at 80°C for 20min, centrifuge at 6000r / min for 40min, and collect the supernatant, which is the extract of Pleurotus ostreatus;
[0022] (3) Enzymolysis I: put the extract of Pleurotus ostreatus in an enzymolysis reactor, and add neutral protease (10 5 U / g), adjust the pH value to 6.0, hydrolyze at 40°C for 4 hours; then treat at 80°C for 20min, inactivate protease, centrifuge at 6000r / min for 40min, collect the supernatant, namely enzymatic solution I;
[0023...
Embodiment 2
[0026] Example 2 Preparation of Pleurotus ostreatus Active Peptide
[0027] (1) Pretreatment: crush the dried fruiting bodies of Pleurotus ostreatus, pass through a 60-mesh sieve, mix with distilled water at a material-to-liquid ratio of 1:25 (W / V), and make a pulp, namely Pleurotus ostreatus slurry;
[0028] (2) Extraction: Put the slippery mushroom slurry in an ultrasonic extractor, control the ultrasonic frequency at 400W, add laccase (10 4 U / g), adjust the pH value to 5.5, and treat at 45°C for 1.5 hours; then treat at 90°C for 15min, centrifuge at 8000r / min for 30min, and collect the supernatant, which is the extract of Pleurotus ostreatus;
[0029] (3) Enzymolysis I: put the extract of Pleurotus ostreatus in an enzymolysis reactor, and add neutral protease (10 5 U / g), adjust the pH value to 6.5, hydrolyze at 45°C for 3 hours; then treat at 90°C for 15 minutes, inactivate protease, centrifuge at 8000r / min for 30 minutes, collect the supernatant, namely enzymatic solution...
Embodiment 3
[0033] Example 3 Preparation of Pleurotus ostreatus Active Peptide
[0034] (1) Pretreatment: Grind the dried fruiting bodies of Pleurotus chinensis, pass through a 60-mesh sieve, mix with distilled water at a material-to-liquid ratio of 1:30 (W / V), and make pulp, that is, Pleurotus chinensis slurry;
[0035] (2) Extraction: put the slippery mushroom slurry in an ultrasonic extractor, control the ultrasonic frequency at 300W, add laccase (10 4 U / g), adjust the pH value to 6.0, and treat at 50°C for 1 hour; then treat at 100°C for 10 minutes, centrifuge at 10,000r / min for 20 minutes, and collect the supernatant, which is the extract of Pleurotus ostreatus;
[0036] (3) Enzymolysis I: put the extract of Pleurotus ostreatus in an enzymolysis reactor, and add neutral protease (10 5 U / g), adjust the pH value to 7.0, hydrolyze at 50°C for 2 hours; then treat at 100°C for 10 minutes, inactivate protease, centrifuge at 10,000r / min for 20 minutes, collect the supernatant, namely enzym...
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