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A metabolizable gold nanosphere/chicken ovalbumin composite nanophotoacoustic probe and its preparation

A gold nanosphere and albumin technology, applied in the field of tumor photoacoustic imaging detection, can solve the problems of high biological toxicity, damage, poor biocompatibility, etc., and achieve the effects of high biological inertness, high economic benefit and low cost

Inactive Publication Date: 2019-11-05
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composite probe has poor biocompatibility, high biotoxicity, and is easy to accumulate in biological tissues and cause damage.

Method used

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  • A metabolizable gold nanosphere/chicken ovalbumin composite nanophotoacoustic probe and its preparation
  • A metabolizable gold nanosphere/chicken ovalbumin composite nanophotoacoustic probe and its preparation
  • A metabolizable gold nanosphere/chicken ovalbumin composite nanophotoacoustic probe and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of a metabolizable gold nanosphere / chicken ovalbumin composite nano-photoacoustic probe comprises the following steps:

[0034] (1) Using 2-(N-morpholine)ethanesulfonic acid (MES for short) solution with a pH of 6, chicken ovalbumin molecules were prepared into a 0.5 mg / ml solution, and stirred evenly.

[0035] (2) 1ml of gold nanosphere solution with a concentration of 1mg / ml was slowly added dropwise to 2ml of chicken ovalbumin solution in step (1), and stirred for 1h at a stirring speed of 450rpm. Gold nanospheres are specifically made by the following method: 0.025mmol of tetraoctylammonium bromide and 0.06mmol of dodecylamine are dissolved in 0.5ml of toluene, then 0.053mmol of chloroauric acid aqueous solution (concentration is 0.5M) is added, Mix well, then add 0.2 mmol of ice-cold sodium borohydride, stir vigorously for half an hour, and finally add 4 ml of poor solvent ethanol, collect the precipitate to obtain dodecaline-modified gold nanospher...

Embodiment 2

[0045] The preparation of a metabolizable gold nanosphere / chicken ovalbumin composite nano-photoacoustic probe comprises the following steps:

[0046] (1) Using 2-(N-morpholine)ethanesulfonic acid (MES for short) solution with a pH of 6, chicken ovalbumin molecules were prepared into a 0.5 mg / ml solution, and stirred evenly.

[0047] (2) 0.2ml of gold nanosphere solution (preparation method is the same as in Example 1) with a concentration of 0.5mg / ml was slowly added dropwise to 2ml of chicken ovalbumin solution in step (1), stirred for 2h, and the stirring speed was 450rpm .

[0048] (3) Put the mixed solution in step (2) into a probe-type ultrasonic instrument, and perform an ultrasonic reaction for 20 minutes (ultrasonic power 400W, working time 10s, ultrasonic interval 5s), and finally obtain a gold nanosphere / chicken ovalbumin composite nanometer Hybrid systems of photoacoustic probes. During the sonication process, the solution was placed in an ice-water bath to preve...

Embodiment 3

[0053] The preparation of a metabolizable gold nanosphere / chicken ovalbumin composite nano-photoacoustic probe comprises the following steps:

[0054] (1) Using 2-(N-morpholine)ethanesulfonic acid (MES for short) solution with a pH of 6, chicken ovalbumin molecules were prepared into a 0.1 mg / ml solution, and stirred evenly.

[0055] (2) 2ml of gold nanosphere solution with a concentration of 1mg / ml (preparation method is the same as in Example 1) was slowly added dropwise to 2ml of chicken ovalbumin solution in step (1), and stirred for 0.5h at a stirring speed of 200rpm.

[0056] (3) Put the mixed solution in step (2) into a probe-type ultrasonic instrument, and ultrasonically react for 5 minutes (ultrasonic power 100W, working time 20s, ultrasonic interval 3s), and finally obtain a gold nanosphere / chicken ovalbumin composite nanometer Hybrid systems of photoacoustic probes. During the sonication process, the solution was placed in an ice-water bath to prevent the solution ...

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Abstract

The invention relates to an easily metabolized gold nano-particle ball / chicken egg albumin compound nanometer photo-acoustic probe and a preparation thereof. The compound nanometer photo-acoustic probe is formed by compounding the gold nano-particles modified with 12-ammonia and the chicken egg albumin molecules wrapping the gold nano-particles, wherein the mass ratio of the gold nano-particles to the chicken egg albumin molecules is (0.1-10):1. Compared with the prior art, the compound nanometer photo-acoustic probe provided by the invention has a photo-acoustic effect under the irradiation of 600-950nm pulse optical wave; the content of glutathione in a tumor microenvironment is higher, so that the gold nano-particle ball / chicken egg albumin compound nanometer photo-acoustic probe can be reduced in tumor tissue and can release the gold nano-particles; the gold nano-particles in small size at 5nm level can be metabolized through kidneys in body circulation, so that the gold nano-particles are prevented from accumulating and causing injury in the biological tissue.

Description

technical field [0001] The invention relates to the field of tumor photoacoustic imaging detection, in particular to a metabolizable gold nanosphere / chicken ovalbumin composite nano photoacoustic probe and its preparation. Background technique [0002] Photoacoustic imaging is an emerging biodetection technology. Because of its high spatial resolution and imaging depth, real-time dynamic imaging, and simple and convenient operation, it has great potential in clinical applications. In photoacoustic imaging tests, the signal strength of photoacoustic probes plays a key role in imaging quality, so the research and development of photoacoustic probes with super-strong photoacoustic signals has attracted extensive attention. According to the source, photoacoustic probes are divided into endogenous photoacoustic probes and exogenous photoacoustic probes. Endogenous photoacoustic probes are derived from living organisms and have good biocompatibility and low biotoxicity. Exogenous...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B5/00
CPCA61B5/0095
Inventor 刘萍李永勇任天斌董海青
Owner TONGJI UNIV
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