Natural high-polymer bioactive trauma repair material with function of releasing active ions and method for preparing natural high-polymer bioactive trauma repair material

A technology of polymer biology and natural polymer, applied in the field of biomedical materials, can solve problems such as difficult to use patch materials, failure to enter the composite phase, and damage to the structure of the polymer matrix

Active Publication Date: 2017-09-26
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the traditional way of preparing inorganic bioceramic-polymer composites includes stirring and mixing bioceramic powder with liquid polymer and casting, but this method is difficult to be used in the preparation of patch materials, because mechanical effects will destroy Structure of polymer matrix (Pharm.Res.2008,25,1222.)
Other methods include compounding the two in the form of su

Method used

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  • Natural high-polymer bioactive trauma repair material with function of releasing active ions and method for preparing natural high-polymer bioactive trauma repair material
  • Natural high-polymer bioactive trauma repair material with function of releasing active ions and method for preparing natural high-polymer bioactive trauma repair material
  • Natural high-polymer bioactive trauma repair material with function of releasing active ions and method for preparing natural high-polymer bioactive trauma repair material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] (1) Preparation of calcium silicate leaching solution:

[0064] The original solution of CS extract was diluted 64, 16, and 4 times with cell culture medium, shaken evenly at room temperature, and then sterilized through a 0.22mm filter, and stored in a refrigerator at 4°C for future use.

[0065] (2) Preparation of calcium silicate-decellularized myocardial matrix composite material:

[0066] The decellularized myocardial matrix with a diameter of 10 mm was submerged in 300 μL of calcium silicate extracts with dilution factors of 64, 16, and 4 times, respectively. Place in a cell culture incubator at 37 degrees, 5% CO 2 Soak for 48 hours under the environment. Then the composite material was taken out, placed in a minus 80 degree refrigerator to freeze, and finally freeze-dried for 24 hours.

[0067] The test results of ion release showed that ( figure 1), in each group, calcium and silicon ions could be released stably within 7 days, and the daily release amount w...

Embodiment 2

[0069] (1) Preparation of calcium silicate leaching solution:

[0070] Dilute the stock solution of CS extract with cell culture medium by 64, 16, and 4 times respectively, shake it evenly at room temperature, and then pass it through a 0.22mm filter to sterilize it, store it in a refrigerator at 4°C, and store it for later use.

[0071] (2) Preparation of calcium silicate-adcellular dermal matrix composite material:

[0072] The acellular dermal matrix with a diameter of 10 mm was submerged in 300 μL calcium silicate extraction solution with dilution factors of 64, 16 and 4 times respectively. Place in a cell culture incubator at 37 degrees, 5% CO 2 Soak for 48 hours under the environment. Then the composite material was taken out, placed in a minus 80 degree refrigerator to freeze, and finally freeze-dried for 24 hours to obtain a calcium silicate-decellularized dermal matrix composite material.

Embodiment 3

[0074] (1) Preparation of calcium silicate leaching solution:

[0075] The original solution of CS extract was diluted 4 times with cell culture medium, shaken evenly at room temperature, sterilized through a 0.22mm filter, and stored in a refrigerator at 4°C for later use.

[0076] (2) Preparation of calcium silicate-collagen membrane composite material:

[0077] Submerge the 10 mm diameter collagen membrane in 300 μL calcium silicate extract and stock solution with a dilution factor of 4. Place in a cell culture incubator at 37 degrees, 5% CO 2 Soak for 48 hours under the environment. Then the composite material was taken out, placed in a minus 80 degree refrigerator to freeze, and finally freeze-dried for 24 hours.

[0078] The test results of ion release showed that ( Image 6 ), the release of calcium and silicon ions in each group was more on the first day, which were 21.0ppm and 12.9ppm respectively, and then gradually decreased, and the average release amount and c...

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Abstract

The invention relates to a natural high-polymer bioactive trauma repair material with a function of releasing active ions and a method for preparing the natural high-polymer bioactive trauma repair material. The method includes soaking natural high-polymer materials in silicate extract liquid for a period of time; taking the natural high-polymer materials out of the silicate extract liquid and drying the natural high-polymer materials to obtain the natural high-polymer bioactive trauma repair material with the function of controllably releasing the ions. The natural high-polymer bioactive trauma repair material and the method have the advantages that the natural high-polymer bioactive trauma repair material is creatively prepared by the aid of silicate extract liquid permeating processes, aqueous solution is used as a medium, silicate components with angiogenesis activity can be uniformly dispersed inside the natural high-polymer materials, granular agglomeration can be prevented, and accordingly the natural high-polymer bioactive trauma repair material which is a bioactive composite trauma repair material can be prepared; the inorganic ions are uniformly adsorbed and distributed in high-polymer matrixes, and the ion release concentration can be controlled.

Description

technical field [0001] The invention relates to a method for preparing a natural macromolecule bioactive wound repair material with controllable release of ions by an infiltration method of a silicate bioceramic extract, belonging to the field of biomedical materials. Background technique [0002] Injuries to soft tissues or organs caused by various traumas, burns, operations, and insufficient blood supply are common clinical diseases. If the surface tissue of the human body such as the skin is damaged in a large area, it will cause local or even systemic problems such as excessive loss of water and protein, increased metabolism, and immune system imbalance; internal tissue such as the myocardium, if the necrosis of the myocardium is caused by ischemia, May deteriorate leading to heart failure, and myocardial tissue is difficult to regenerate (J. Foot Ankle Surg. 1997, 36, 2.; Am. J. Cardiol. 2012, 109, 187.). According to statistics, the cost of global trauma treatment is ...

Claims

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Application Information

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IPC IPC(8): A61L27/44
CPCA61L27/446A61L2300/412A61L2300/602A61L2430/00
Inventor 常江王晓彤
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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