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Method for screening molecular markers related to spirodela oligorrhiza root growth

A molecular marker, root growth technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effect of improving biological treatment efficiency and fast detection speed

Active Publication Date: 2017-09-26
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection method of the invention has the advantages of rapidity, accuracy, sensitivity, etc., and can intuitively detect the genotypes of different individuals of the rusty spot, so as to quickly obtain the polymorphism map of the genetic variation of the functional gene microsatellite locus of the rusty spot, the microsatellite Markers can be applied to the fields of genetic variation and population genetic diversity analysis of rust spot moth, but there is still room for improvement in the detection speed of microsatellite markers

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  • Method for screening molecular markers related to spirodela oligorrhiza root growth
  • Method for screening molecular markers related to spirodela oligorrhiza root growth

Examples

Experimental program
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Effect test

Embodiment 1

[0015] The method for screening the molecular markers related to the growth of Pygnus chinensis includes clone culture, genomic DNA extraction, design of microsatellite primers, PCR amplification, and data statistics and analysis.

[0016] The composition and concentration of the nutrient solution for plant clone culture are: nitrogen 0.3~0.4g / L, phosphoric anhydride 0.1~0.15 g / L, potassium oxide 0.16~0.23 g / L, magnesium oxide 0.01~0.015 g / L and sulfur 0.012 ~0.02 g / L. The above-mentioned nutrient solution can comprehensively provide the macroelements and trace elements required for the growth of P. chinensis, the asexual reproduction speed is fast, the probability of gene mutation is low, and a large number of plants with the same gene can be obtained, and the sample size is sufficient.

[0017] The plant clones were cultured indoors in a light incubator for 50-70 days, the culture conditions were 22.5-23.5°C, the humidity was 73-83%, the light intensity was 1800-2200lux, and...

Embodiment 2

[0022] The method for screening the molecular markers related to the growth of Pygnus chinensis includes clone culture, genomic DNA extraction, design of microsatellite primers, PCR amplification, and data statistics and analysis.

[0023] The composition and concentration of the nutrient solution for plant clone cultivation are: nitrogen 0.32 g / L, phosphoric anhydride 0.12 g / L, potassium oxide 0.19 g / L, magnesium oxide 0.012 g / L and sulfur 0.016 g / L. The above-mentioned nutrient solution can comprehensively provide the macroelements and trace elements required for the growth of P. chinensis, the asexual reproduction speed is fast, the probability of gene mutation is low, and a large number of plants with the same gene can be obtained, and the sample size is sufficient.

[0024] The plant clones were cultivated indoors for 60 days in a light incubator, the culture conditions were 23°C, humidity 78%, light intensity 2000lux, and light-dark time 16:8h. Under the above-mentioned ...

Embodiment 3

[0029] The method for screening the molecular markers related to the growth of Pygnus chinensis includes clone culture, genomic DNA extraction, design of microsatellite primers, PCR amplification, and data statistics and analysis.

[0030] The composition and concentration of the nutrient solution for plant clone cultivation are: nitrogen 0.32g / L, phosphoric anhydride 0.12g / L, potassium oxide 0.2g / L, magnesium oxide 0.013g / L and sulfur 0.017g / L. The above-mentioned nutrient solution can comprehensively provide the macroelements and trace elements required for the growth of P. chinensis, the asexual reproduction speed is fast, the probability of gene mutation is low, and a large number of plants with the same gene can be obtained, and the sample size is sufficient.

[0031] The number of roots / piece, total root length / piece and average root length / piece were counted for each plant (leaves connected) in different culture lines.

[0032] Microsatellite primers are: Sp16; F: GGATC...

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Abstract

The invention discloses a method for screening molecular markers related to spirodela oligorrhiza root growth. The method for screening the molecular markers related to the spirodela oligorrhiza root growth, disclosed by the invention, comprises the following steps: cloning body culture, genome DNA extraction, microsatellite primer design, PCR amplification and data statistics and analysis. The method disclosed by the invention has the benefits that DNA sequences of a large amount of microsatellite primer-marked spirodela oligorrhiza can be quickly obtained, the microsatellite marking detection speed is high, and spirodela oligorrhiza Sp16, Sp51 and Sp55 genetic marker loci can be quickly obtained to show a polymorphic map with high genetic variation. The total root length / piece and the average root length / piece of the spirodela oligorrhiza are significantly related to Sp16, Sp51 and Sp55 (P is less than 0.001). The effect of the genotype variation on spirodela oligorrhiza growth and pollutant purification capacity is understood, so that the biological treatment efficiency of wastewater is improved.

Description

technical field [0001] The invention relates to the technical field of DNA molecular markers, in particular to a method for screening molecular markers related to the growth of P. Background technique [0002] Rich, highly heterozygous and good stability, co-dominant inheritance following Mendelian segregation law, easy PCR amplification, etc., have become widely used DNA markers in recent years, and are often used in family pedigree authentication and gene linkage analysis of biological resources , genetic map construction, germplasm identification, population genetic diversity and many other research fields. [0003] In the natural environment, P. spp. basically reproduces asexually. When the environmental conditions are suitable, one generation can be cloned in 2 days. The genetic diversity of the population is low. [0004] The prior art provides a variety of detection methods for satellite markers, for example, Chinese Invention Authorized Patent Document A Rapid Detec...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 徐娜娜徐嘉俊朱旭辉
Owner ZHEJIANG OCEAN UNIV
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