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Construction system and method of 3D (three-dimensional) micro/nano-scale prefabricated vessel network of bone tissues

A micro-nano scale and construction method technology, applied in tissue regeneration, additive manufacturing, pharmaceutical formulations, etc., can solve problems that cannot be effectively solved, and achieve the effect of easy materials, high flexibility, and simple preparation process

Active Publication Date: 2017-10-27
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, this method cannot effectively solve the problem of prefabricated vascularized network of bone tissue at the micro-nano scale

Method used

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  • Construction system and method of 3D (three-dimensional) micro/nano-scale prefabricated vessel network of bone tissues
  • Construction system and method of 3D (three-dimensional) micro/nano-scale prefabricated vessel network of bone tissues
  • Construction system and method of 3D (three-dimensional) micro/nano-scale prefabricated vessel network of bone tissues

Examples

Experimental program
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Effect test

Embodiment 1

[0031] In this example, see Figure 1 ~ Figure 3 , a method for constructing a three-dimensional micro-nano-scale prefabricated vascular network of bone tissue, comprising the following steps:

[0032] a. Use the blend of PVA aqueous solution and chitosan solution as the sacrificial material, use absolute ethanol as the catalyst, and the aluminum alloy round shaft that can coordinate the movement as the receiving device, connect the positive electrode of the high-voltage power supply to the 3D printing nozzle, and ground The wire is connected to the rotating receiving shaft, and the fiber filament formed when there is voltage connects the nozzle to the rotating receiving shaft to form a closed path, and the direct writing process can be controlled by shortening the distance between the nozzle and the rotating receiving shaft;

[0033]b. Replace the nozzle in step a with a 20G needle, input the coding program of the sacrificial structure into the 3D printer and run it. Under th...

Embodiment 2

[0038] This embodiment is basically the same as Embodiment 1, especially in that:

[0039] In this embodiment, a method for constructing a three-dimensional micro-nano-scale prefabricated vascular network of bone tissue, using PGA as the polymer I material, includes the following steps:

[0040] a. Use the blend of PGA aqueous solution and chitosan solution as the sacrificial material, use absolute ethanol as the catalyst, and the aluminum alloy round shaft that can coordinate the movement as the receiving device, connect the positive electrode of the high-voltage power supply to the 3D printing nozzle, and ground The wire is connected to the rotating receiving shaft, and the fiber filament formed when there is voltage connects the nozzle to the rotating receiving shaft to form a closed path, and the direct writing process can be controlled by shortening the distance between the nozzle and the rotating receiving shaft;

[0041] b. Replace the nozzle in step a with a 20G needle...

Embodiment 3

[0045] This embodiment is basically the same as the foregoing embodiment 1, and the special features are:

[0046] In this embodiment, a method for constructing a three-dimensional micro-nano-scale prefabricated vascular network of bone tissue, the hydrogel uses gelatin loaded with human bone marrow mesenchymal stem cells, including the following steps:

[0047] a. This step is identical with embodiment 1;

[0048] B. this step is identical with embodiment 1;

[0049] c. After the three-dimensional micro-nano-scale sacrificial module prepared in step b is formed, coat a layer of liquid paraffin on its surface, then place it in a 37°C drying oven to dry the paraffin, and configure 15% gelatin with deionized water at the same time, Place in a 60°C water bath and stir until the gelatin particles are uniformly dissolved and the temperature drops to 30°C. At this time, add mTG enzyme (the mass ratio of gelatin to mTG enzyme is 10:1) to cross-link the gelatin solution. When prepari...

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Abstract

The invention discloses a construction system and method of a 3D (three-dimensional) micro / nano-scale prefabricated vessel network of bone tissues, which are used for the field of bio-manufacturing and used for manufacturing a micro / nano-scale prefabricated vessel access structure by combining an electrohydrodynamics direct-writing process and a subtractive manufacturing technology. A 3D shape of a required sacrificial material is prepared by promoting the sacrificial material to form sacrificial material solution with anhydrous ethanol, no harmful substance is generated in the whole process, and the material is easily available. The micro / nano-scale vessel structure is formed by virtue of printability of PVA (Polyvinyl Acetate), PGA (propylene glycol alginate) and chitosan, and the problem that the 3D micro / nano-scale vessel network cannot be obtained through biological 3D printing is solved, therefore, the system and the method have significance for solving the problem of the scale of a vessel in repair of human tissues in clinical medicine.

Description

technical field [0001] The invention relates to a system and method for constructing a three-dimensional micro-nano-scale prefabricated blood vessel network of bone tissue, which is applied in the technical field of biomanufacturing. Background technique [0002] The ultimate goal of tissue engineering is to implant artificial tissues or organs to replace diseased parts in the human body, provide different solutions for tissue or organ transplantation, and improve the quality of life and prolong life activities. Bio-3D printing technology has made great progress in regenerative tissue repair, and a large number of bio-scaffolds have been prepared for clinical application, such as skin, bone, cartilage and nerve. But there are still some technical hurdles to be overcome, one of which is the construction of 3D micro-nanoscale prefabricated vascular networks, especially for tissues and organs with smaller sizes. The vascular network plays a role in delivering nutrients to tiss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/22A61L27/38A61L27/20A61L27/16A61L27/18A61L27/50B29C64/106B29C64/20B29C64/40B33Y10/00B33Y30/00B33Y70/00
CPCA61L27/16A61L27/18A61L27/20A61L27/222A61L27/3834A61L27/50A61L27/52A61L2400/12A61L2430/02B33Y10/00B33Y30/00B33Y70/00C08L5/08C08L29/04C08L67/04
Inventor 胡庆夕孙程艳刘媛媛李帅李冬冬
Owner SHANGHAI UNIV
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