Compound staining solution of piroplasma blood smear and staining method of blood smear

A dyeing method and blood smear technology, applied in the biological field, can solve the problems of affecting insect bodies, complex dyeing methods, and long time, and achieve the effect of short time, good dyeing effect, and outstanding effect
CN107300495AInactive Publication Date: 2017-10-27托克逊县夏乡畜牧业服务中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
托克逊县夏乡畜牧业服务中心
Publication Date
2017-10-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a compound staining solution of a piroplasma blood smear. The compound staining solution comprises a compound staining solution I and a compound staining solution II, wherein the compound staining solution I comprises 2g Swiss powder, 1.7g giemsa pigment, 50ml glycerol and 1000ml methanol; and the compound staining solution II comprises 2.3g anhydrous sodium dihydrogen phosphate, 0.3g anhydrous monopotassium phosphate and 1000ml distilled water. The staining method comprises the following steps: I, blood smear preparation, II, secondary treatment of the blood smear, and III, staining in the compound staining solution. The compound staining solution and the staining method have a better piroplasma staining effect without the help of high-end instruments and equipment, are economical and practical and have a very excellent effect; the required time is shorter; and no impurity interferes with observation of a piroplasma body.
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Description

technical field

[0001] The invention belongs to the field of biological technology, and in particular relates to a composite dye solution for blood smears of piriformis and a dyeing method for the blood smears. Background technique

[0002] Piroplasmosis (piroplasmosis), also known as "pyroplasmosis" or "blood sporidiosis". is a group of hard-transmitted blood parasitic protozoa diseases, including Theileria ( Theileria app ) and Babesia ( Babesia app ) is the general term for blood protozoan diseases caused by It is more common in cattle, horses, sheep and other domestic animals, rodents and other wild animals. Clinically, symptoms such as persistent fever, anemia, dyspnea, jaundice, etc. appear in this disease. If the diagnosis and treatment are not timely, the mortality rate is extremely high. It is widely prevalent and causes huge losses to animal husbandry and the national economy. According to the statistics of the United Nations Food and Agriculture Organization (...

Claims

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