Compound staining solution of piroplasma blood smear and staining method of blood smear

A dyeing method and blood smear technology, applied in the biological field, can solve the problems of affecting insect bodies, complex dyeing methods, and long time, and achieve the effect of short time, good dyeing effect, and outstanding effect

Inactive Publication Date: 2017-10-27
托克逊县夏乡畜牧业服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is mentioned in the relevant literature that blood smears are used to stain the parasites. The staining method is too complicated, takes a long time, and the staining effect is not ideal, and there are many staining impurities, which affect the observation of parasites.

Method used

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  • Compound staining solution of piroplasma blood smear and staining method of blood smear
  • Compound staining solution of piroplasma blood smear and staining method of blood smear
  • Compound staining solution of piroplasma blood smear and staining method of blood smear

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1, a kind of composite dye solution of Piroplasma blood smear, described composite dye solution comprises composite dye solution I and compound dye solution II; Described composite dye solution I is made of Wright's dye 2g, Giemsa pigment 1.7 g. Composed of 50ml glycerin and 1000ml methanol. The specific preparation method is: add 2g of Wright's dye and 1.7g of Giemsa pigment into a mortar, add 50ml of glycerin to grind into a uniform paste, add 1000ml of methanol, grind and collect the upper dye solution for storage Put it in a brown bottle; the composite dye solution II is composed of 2.3 g of anhydrous sodium dihydrogen phosphate, 0.3 g of anhydrous potassium dihydrogen phosphate and 1000 ml of distilled water. The specific preparation method is: anhydrous dihydrogen phosphate Sodium 2.3g and anhydrous potassium dihydrogen phosphate 0.3g are dissolved in 1000ml of distilled water to prepare a phosphate buffer solution with a pH value of 6.5-6.8.

Embodiment 2

[0013] Embodiment 2, a kind of staining method of piriformis blood smear, this staining method comprises the following steps:

[0014] 1. Use a pipette to absorb 20 μl of blood drop with piriformis, and drop it onto the end of slide A. Take another piece of slide B with smooth edges as a pusher, place it in the center of the blood drop, and let the blood flow along the slide. Spread the edge of B evenly, and then place slide B at 45° on the blank space of slide A and push the slide back, leaving a thin layer of blood film on slide A; place it at room temperature after successful push to make the blood film Dry, pay attention to the thickness of the blood film when smearing, the distribution of red blood cells should be uniform, to ensure that the red blood cells do not accumulate, and at the same time there should be no gaps on the blood film, spare;

[0015] 2. Place the blood smear prepared in step 1 at room temperature for 5 minutes. After the blood is completely air-dried,...

Embodiment 3

[0017] Embodiment 3, the blood smear prepared by using the above technical method is as attached figure 1 , attached figure 2 and attached image 3 As shown, the piriformis can be clearly observed under the microscope.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a compound staining solution of a piroplasma blood smear. The compound staining solution comprises a compound staining solution I and a compound staining solution II, wherein the compound staining solution I comprises 2g Swiss powder, 1.7g giemsa pigment, 50ml glycerol and 1000ml methanol; and the compound staining solution II comprises 2.3g anhydrous sodium dihydrogen phosphate, 0.3g anhydrous monopotassium phosphate and 1000ml distilled water. The staining method comprises the following steps: I, blood smear preparation, II, secondary treatment of the blood smear, and III, staining in the compound staining solution. The compound staining solution and the staining method have a better piroplasma staining effect without the help of high-end instruments and equipment, are economical and practical and have a very excellent effect; the required time is shorter; and no impurity interferes with observation of a piroplasma body.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a composite dye solution for blood smears of piriformis and a dyeing method for the blood smears. Background technique [0002] Piroplasmosis (piroplasmosis), also known as "pyroplasmosis" or "blood sporidiosis". is a group of hard-transmitted blood parasitic protozoa diseases, including Theileria ( Theileria app ) and Babesia ( Babesia app ) is the general term for blood protozoan diseases caused by It is more common in cattle, horses, sheep and other domestic animals, rodents and other wild animals. Clinically, symptoms such as persistent fever, anemia, dyspnea, jaundice, etc. appear in this disease. If the diagnosis and treatment are not timely, the mortality rate is extremely high. It is widely prevalent and causes huge losses to animal husbandry and the national economy. According to the statistics of the United Nations Food and Agriculture Organization (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/28
CPCG01N1/2813G01N1/30G01N2001/302
Inventor 海尼木古力·艾合买提张杨巴音查汗·盖力克刘世芳郭庆勇谢小婉王盼举闻秀秀宋瑞其
Owner 托克逊县夏乡畜牧业服务中心
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