Method for preparing high-activity ginsenoside by solid dynamic fermentation technology

A ginsenoside, dynamic fermentation technology, applied in the field of ginsenoside, can solve the problems of cumbersome process, low conversion rate of low-glycosyl saponin, complicated operation and the like

Inactive Publication Date: 2017-11-03
肖永坤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the use of complex bacteria for fermentation, the optimal culture conditions for each type of bacteria are different, and it is also difficult to ensure that the number ratio of each type of bacteria is consistent in each culture process, which greatly increases the difficulty of the culture process and the fermentation products are uncertain. , many by-products, low conversion rate of low glycosyl saponins, etc.
It is

Method used

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  • Method for preparing high-activity ginsenoside by solid dynamic fermentation technology
  • Method for preparing high-activity ginsenoside by solid dynamic fermentation technology
  • Method for preparing high-activity ginsenoside by solid dynamic fermentation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Preparation of fermented bacteria seeds: Take 300g of ginseng root and crush it to 10-20 mesh, mix it with 900g of wheat bran, add 1L of water, stir evenly, put it into a triangular flask, sterilize at 120°C for 20 minutes, and put it into the research team for screening after cooling down A kind of Aspergillus niger, which was produced, was cultured at 28°C for 7-8 days to obtain fermented seeds.

[0062] Large-scale fermentation: Take 50kg of ginseng root, crush it to 10-20 mesh, mix it with 50kg of wheat bran, 20kg of soybean meal, and 80kg of water, put it into the fermentation tank, stir evenly, steam heat to 100°C for sterilization, and cool it down rapidly after 10 minutes to 40°C, and then insert the prepared seeds. The fermenter is always fed with filtered sterile air, the temperature of the interlayer of the fermenter is set at 30°C, and after 30 hours of heat preservation, the product temperature is kept between 28-30°C. After 10 days of fermentation, the fe...

Embodiment 2

[0064] Fermented seeds were prepared according to the method in Example 1.

[0065] Take 30kg of ginseng root, crush it to 10-20 mesh, mix it with 70kg of wheat bran, 20kg of Sophora japonica flower, and 70kg of water, put it into a fermenter, stir evenly, steam heat to 100°C for sterilization, and after 10 minutes, rapidly cool to 40°C ℃, and then insert the prepared seeds. The fermenter is always fed with filtered sterile air, the temperature of the interlayer of the fermenter is set at 30°C, and after 30 hours of heat preservation, the product temperature is kept between 28-30°C. After 12 days of fermentation, the fermentation product is rich in F2 and CK, such as figure 2 shown.

[0066] Then add 500L methanol to the fermenter, extract 3 times at 50°C for 3 hours each time, concentrate the extract under reduced pressure at 25~70°C and -0.01Mpa~1Mpa until no methanol remains, add 50L cyclohexane to degrease twice, Put the degreased solution on a 30LAB-8 macroporous adso...

Embodiment 3

[0068]In Example 2, 116 kg of the slag soaked in methanol was extracted, and 400 L of water was added to it, and the ginsenoside hydrolase was extracted at 70° C. for 6 hours, extracted twice, and the extracts were combined, concentrated at 55° C. to reach 52 Brix, and 11.8 kg of active ginseng was obtained. Hydrolase solution. Get F2 10g obtained in Example 2, dissolve in 200mL water, add 20mL acetic acid, mix with 200g active ginseng hydrolase solution, react at 80°C for 4h, add 800mL deionized water, the product is Rh2 group saponin, i.e. 20( R)-Rh2, 20(S)-Rh2, Rk2 and Rh3, and saponins. Extracted 3 times with water-saturated n-butanol, combined the n-butanol layers, evaporated to dryness to obtain 5.8g of powder, and the TLC detection results were as follows: Image 6 shown.

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Abstract

The invention discloses a method for preparing high-activity ginsenoside by a solid dynamic fermentation technology and particularly relates to a high-activity ginseng product prepared by using plant tissues of panax species as a solid culture medium, inoculating with a single aerobic strain which is selected and can generate ginsenoside hydrolase and carrying out dynamic fermentation and rich in C-K and F2. The ginseng product is extracted and separated to obtain a C-K and F2 mixed saponin or monomer saponin; or a mixture or a monomer of the C-K, Rh2 and diol saponins can be further prepared by using the hydrolase extracted from the high-activity ginseng product. According to the method, high-activity underglycosylated ginsenoside can be obtained by directly using the plant tissues of the panax species as a raw material for fermenting without preparation of original diol saponin PPD (Paraphenylenediamine) or special preparation of panaxsaponin glucosidase. The method has the advantages of few process steps, low production cost, no pollution and suitability for industrial production.

Description

technical field [0001] The invention relates to the field of ginsenosides, in particular to a method for preparing highly active ginsenosides through solid dynamic fermentation technology. Background technique [0002] The ginseng plants grown in my country are mainly ginseng from Northeast China, Sanqi ginseng from Yunnan and Guangxi, and American ginseng from Shandong and Jilin. There are also a small amount of bamboo ginseng and pearl ginseng being planted and used. Its main activity depends on the saponins. Saponin is a kind of glycoside connected by glycoside and non-sugar ligand through glycosidic bond. According to the difference between glycosyl and ligand and the position of glycosidic bond, saponin is divided into different types. Different types of saponins are physically and The chemical properties are different. Can be divided into panaxadiol saponins (PPD) according to the difference of structure, such as Rb1, Rb2, Rb3, Rc, Rd, Rg3, Rg5, Rh2, Rh3, F2, C-K etc...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61K36/25A23L33/105A61K8/9789A61Q19/00
CPCA23L33/105A61K8/97A61K36/25A61K36/258A61K2236/19A61K2236/333A61K2236/39A61K2236/51A61K2800/85A61Q19/00
Inventor 肖永坤王东明栾桂花
Owner 肖永坤
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