Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quantum dot immunochromatographic test strip for detecting nitrofurantoin metabolite, preparation method and application thereof

A technology of nitrofurantoin metabolites and immunochromatographic test paper, which is applied in the field of immunoassay, can solve the problems of complex operation, high equipment requirements, and low sensitivity, and achieve the effect of simple operation, high sensitivity, and simple storage

Inactive Publication Date: 2017-11-03
CHONGQING NORMAL UNIVERSITY
View PDF10 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies and defects of the prior art, the present invention utilizes the fluorescence characteristics of multi-wavelength excitation of quantum dots, high-intensity fluorescence emission, narrow emission peak, symmetrical peak shape, and good luminescence stability to provide a novel quantum dot for detecting nitrofurantoin metabolites. The preparation method and application of dot immunochromatographic test strips, according to the relationship between the fluorescent properties of quantum dots and the concentration of nitrofurantoin metabolites, detect metabolites on test strips, and realize the quantification of metabolites detected by quantum dot test strips Detection to solve the problems of low sensitivity, complex operation and high equipment requirements in the existing technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quantum dot immunochromatographic test strip for detecting nitrofurantoin metabolite, preparation method and application thereof
  • Quantum dot immunochromatographic test strip for detecting nitrofurantoin metabolite, preparation method and application thereof
  • Quantum dot immunochromatographic test strip for detecting nitrofurantoin metabolite, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 detects the preparation of the test paper of nitrofurantoin metabolite

[0039] 1 Preparation of microporous fiber membrane

[0040] 1.1 React the nitrofurantoin metabolite with p-carboxybenzaldehyde to prepare the nitrofurantoin metabolite hapten; couple the nitrofurantoin metabolite hapten to the carrier protein to prepare the nitrofurantoin metabolite hapten-carrier protein conjugate; use the nitrofurantoin metabolite hapten - Immune mice with carrier protein conjugates, fuse and screen mouse spleen cells and mouse myeloma cells to obtain hybridoma cell lines secreting nitrofurantoin metabolite-specific monoclonal antibodies, and obtain nitrofurantoin metabolite-specific monoclonal Antibody (anti-NPAHD McAb).

[0041] 1.2 Add 0.120g of CdCl to the round bottom flask 2 .2.5H 2 Dissolve O in 100mL three-distilled water, pass nitrogen gas to deoxygenate 20-30 mim, add 72.7μL thioglycolic acid and dissolve in 200mL three-distilled water, adjust the pH val...

Embodiment 2

[0052] Embodiment 2 detects the application of nitrofurantoin metabolite test paper

[0053] 1 sample pretreatment

[0054] Take (5.0±0.05)g homogeneous sample into a 50mL polystyrene centrifuge tube, add 5mL 10% trichloroacetic acid, then add 100μL derivatization reagent, shake fully for 3min, place at 60°C for 1.5h; take it out and add 1mL 0.5 mol / L dipotassium hydrogen phosphate solution, 1.5mL 2mol / L sodium hydroxide solution and 10mL ethyl acetate, shake for 10s, for samples with high fat content, shake slightly for 8-10 times; centrifuge at room temperature (20-25°C) over 3000g 5min; put 8mL of ethyl acetate into a 10mL dry glass test tube, dry it in a water bath at 50-60°C under nitrogen flow, add 1mL of n-hexane, vortex for 30s with a vortexer, and then add 0.6mL of 0.2mol / L phosphate For the buffer solution, vortex for 10 seconds to mix well; centrifuge at room temperature (20-25°C) for 5 minutes above 3000g, remove the upper organic phase, and take 100 μL of the low...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention provides a quantum dot immunochromatographic test strip for detecting a nitrofurantoin metabolite. The test strip is formed by sequentially sticking a sample pad, a microporous fiber membrane, a reaction membrane and an absorbent pad onto a supporting plate, wherein a nitrofurantoin metabolite-specific antibody-quantum dot probe is arranged on the microporous fiber membrane. A detection method using the quantum dot fluorescent immunochromatographic test strip has the advantages of high sensitivity, strong specificity, low cost, simple operation, short detection time, applicability for various units, simple storage, long shelf life, qualitative and quantitative detection and the like; through use of a nitrofurantoin metabolite-specific monoclonal antibody with highly specificity, the reliability of a detection result is ensured; the method for detecting the nitrofurantoin metabolite by using the test strip is simple, convenient, rapid and accurate.

Description

technical field [0001] The invention relates to the field of immune detection, in particular to a quantum dot immunochromatographic test paper for detecting nitrofurantoin metabolites, a preparation method and an application thereof. technical background [0002] Nitrofurantoin (Nitrofurantion), belonging to nitrofuran antibiotics, is a broad-spectrum antibiotic with good antibacterial effect and pharmacokinetic properties. The drug is effective against most Gram-positive bacteria, Gram-negative bacteria, fungi and All pathogens such as protozoa can be killed. Because of its low price and good effect, it was once widely used in aquaculture. However, long-term studies have proved that nitrofuran drugs and metabolites can cause cancer, gene mutation and teratogenic side effects in experimental animals. Many countries have banned the use of such drugs in treatment and feed. In 1995, the EU comprehensively banned the use of nitrofuran antibacterial drugs, and the limit value of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/577G01N33/558
Inventor 乐涛孙琦张磊张志浩
Owner CHONGQING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com