Streptococcus salivarius strain and application thereof

A technology of Streptococcus salivarius and Streptococcus mutans, which is applied in the direction of bacteria, medical preparations containing active ingredients, biochemical equipment and methods, etc., can solve the problems of inapplicable anterior teeth treatment, etc., to broaden industrialized resources and reduce The effect of forming quantity and broadening the application prospect

Pending Publication Date: 2017-11-07
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few drugs under control at present, and silver ammonium nitrate is one of the few. Silver ammonium nitrate is a kind of antiseptic and bactericidal drug,

Method used

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  • Streptococcus salivarius strain and application thereof
  • Streptococcus salivarius strain and application thereof
  • Streptococcus salivarius strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1Screening, identification and preservation of Streptococcus salivarius strains

[0032] The streptococcus salivarius (Streptococcus salivarius) strain in the present invention is obtained by the following way: take 1 mL of oral saliva of normal people with a sterile pipette, serially dilute with sterile physiological saline, and spread the diluted solution evenly on sterile On the M17 agar medium (purchased from OXOID LTD., UK), anaerobic culture at 37°C for 24h, the colonies with smooth and obvious protrusions were selected, and transferred to the M17 liquid medium containing 1% (w / v) sucrose , cultured anaerobically at 37° C. for 24 hours, centrifuged the fermentation broth at 9000 rpm for 10 minutes, and collected the precipitated part (bacteria) and the supernatant part respectively. After the precipitated part was washed twice with sterile distilled water, a small amount of thalline was picked with an inoculation needle and planted on a BHI agar plate (p...

Embodiment 2

[0036] Example 2 Inhibitory effect of Streptococcus salivarius CGMCC NO.13308 on the growth and biofilm formation of Streptococcus mutans

[0037] 1. Materials and methods

[0038] (1) Preparation of seeds (fermentation strains):

[0039] Preparation of Streptococcus salivarius CGMCC No.13308 seed liquid: Dissolve the lyophilized powder of Streptococcus salivarius CGMCC No.13308 with a small amount of sterile distilled water, and use an inoculation loop to get a ring and streak it on M17 solid medium (purchased from OXOID Co. , UK), anaerobic culture at 37°C for 24 hours, and a single colony was picked with an inoculation loop and put into 10 mL M17 liquid medium (purchased from OXOID Co., UK), and the colony was evenly dispersed in the liquid medium by using a vortex shaker, 37 Take out after 24 hours of anaerobic culture at ℃, inoculate in M17 liquid medium (purchased from OXOID Co., UK) with 2% (v / v) inoculum, after 24 hours of anaerobic culture at 37 ℃, centrifuge the c...

Embodiment 3

[0047] Example 3 Inhibitory effect of Streptococcus salivarius CGMCC No.13308 on the growth and biofilm formation of Streptococcus mutans

[0048] 1, material and method: with embodiment 2

[0049] 2. The inhibitory effect of Streptococcus salivarius CGMCC No.13308 on the growth and biofilm formation of Streptococcus mutans

[0050] Streptococcus salivarius seeds were aseptically inoculated in M17 liquid medium containing 5% (w / v) sucrose at an inoculum size of 1% (v / v), and cultured anaerobically at 42° C. for 24 hours to obtain a fermentation broth.

[0051] The fermentation broth was centrifuged at 10000 g for 10 min, and the precipitated part (bacteria) and the fermentation supernatant part were taken respectively. Wherein the precipitation part was washed twice with sterile distilled water, and its inhibitory activity to Streptococcus mutans (expressed as the diameter of the inhibition zone) was determined by the above-mentioned spot method. Wherein the fermentation s...

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Abstract

The invention provides a Streptococcus salivarius strain whose preservation number is CGMCC NO. 13308. The invention further provides application of the Streptococcus salivarius CGMCC NO. 13308 in preparation of a Streptococcus mutans growth inhibitor and a Streptococcus mutans biomembrane formation inhibitor. The new Streptococcus salivarius CGMCC NO. 13308 is disclosed for the first time, cells of the Streptococcus salivarius CGMCC NO. 13308 have the function of inhibiting growth of Streptococcus mutans, and the M17 fermented supernatant fluid can obviously reduce the formation amount of Streptococcus mutans biomembrane. By means of the Streptococcus salivarius strain and its application, the application prospects and industrialized resources of Streptococcus salivarius in the aspect of dental caries are broadened.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Streptococcus salivarius strain and its application in the preparation of Streptococcus mutans growth and biofilm formation inhibitors. Background technique [0002] Dental caries, commonly known as caries, tooth decay, is a bacterial disease, can be secondary to pulpitis and periodontitis, and even cause alveolar bone and jaw inflammation. If it is not treated in time, the lesion will continue to develop, forming cavities, and eventually the crown will be completely destroyed and disappeared. The final result of its development is tooth loss. Dental caries is characterized by high incidence and wide distribution. The disease is the main common disease of the oral cavity and one of the most common human diseases. The World Health Organization has listed it as one of the three major diseases of human beings, along with tumors and cardiovascular diseases. [0003] There...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/744A61P1/02C12R1/46
CPCA61K2035/115C12N1/205C12R2001/46
Inventor 吴正钧于亚男韩瑨冯华峰
Owner BRIGHT DAIRY & FOOD
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