Preparation method of pancreatin

Abstract

The invention relates to the field of biological medicines, and discloses a preparation method of pancreatin. The preparation method comprises the following steps: taking freshly frozen raw materials, namely, freshly frozen pancreas and / or duodenum; carrying out slicing and freeze-drying, namely, slicing the freshly frozen raw materials, and drying the slices by adopting a freeze-drying machine till the water content in the raw materials is less than 8%; carrying out grinding for obtaining powder, namely, grinding the freeze-dried raw materials into freeze-dried powder; carrying out degreasing, namely, degreasing the freeze-dried powder, and carrying out draining to obtain degreased freeze-dried powder; carrying out activating and extracting, namely, adding an activating agent and an extracting solvent into the degreased freeze-dried powder, carrying out stirring for extracting at 0-5 DEG C for 3-6 h, thus obtaining an extracted solution; and carrying out precipitating, namely, carrying out freeze centrifugation on the extracted solution at 0-5 DEG C, taking the supernate, carrying out salting-out, and carrying out separating to obtain pancreatin sediment; carrying out squeezing and drying, namely, squeezing the pancreatin sediment till the pancreatin sediment is block-shaped, thus obtaining pancreatin blocks; drying the pancreatin blocks, and grinding the dried pancreatin blocks to obtain pancreatin powder. According to the preparation method of pancreatin, the yield of the pancreatin product is relatively high, the consumption of the solvent is greatly reduced, the activity of trypsin, amylopsin and pancrelipase in the prepared pancreatin is relatively high, and the yield of pancreatin is relatively high.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a preparation method of pancreatin. Background technique [0002] Pancreatin is a digestive drug recorded in the pharmacopoeias of various countries. It is a mixture of various enzymes extracted from animal pancreas. The main components are trypsin, pancreatic lipase and pancreatic amylase. Pancreatin is mainly used for indigestion, loss of appetite, and digestive disorders caused by liver and pancreatic diseases. It is also suitable for congenital pancreatic insufficiency, abdominal surgery, and pancreatic insufficiency caused by traumatic pancreatectomy. In addition, pancreatin contains a variety of active substances, which can be used as basic raw materials to extract a variety of biochemical drugs, such as pancreatic kininogen, elastase, asparaginase, chymotrypsin, carboxypeptidase, etc., which have great industrial value . [0003] At present, pancreatin is mainly produced throu...

AI Technical Summary

Problems solved by technology

(1) This patent directly uses thawed fresh pancreas or duodenum as raw material, which is activated and extracted after shaving and refining. At this time, the pancreas slurry contains a lot of water, so it is directly activated and extracted. Extraction requires a large amount of activator and extraction solvent, which increases production costs; (2) The pancreas slurry contains a large amount of oil because it has not been degreased. At this time, the activator and extraction solution are directly added for extraction. The existence of oil is not conducive to The penetration of the activator and the extract affects the extraction efficiency, and it is not conducive to the low-temperature extraction to protect the activity of trypsin; (3) the organic solvent acetone is used as the extraction solvent and the precipitation solvent when the trypsin is precipitated, and the patent is still extracted. After the trypsin is degreased with an organic solvent (acetone), the consumption of the organic solvent is large, and the organic solvent will inevitably affect the activity of the trypsin, and the amount of waste liquid is large; (4) the trypsin product in this patent The main index components trypsin activity is only about 3500u / g, pancreatic amylase activity is only about 100000u / g, pancreatic lipase activity is only about 42000u / g, the activity is low