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Microbial preparation for improving tobacco quality

A microbial preparation and quality technology, applied in the field of microorganisms, can solve the problems of poor ability to improve aroma and tobacco quality, unable to reduce the content of harmful substances, and poor ability to degrade nitrite, so as to speed up the reproduction speed, reduce fermentation costs, The effect of enhancing the activity

Active Publication Date: 2017-11-24
湖北京晟生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese patent technology "A Composite Aroma-Producing Microorganism and Its Preparation Method and Application" discloses a microorganism that improves aroma. The microbial preparation is used in tobacco stems and cannot reduce harmful substances such as nitrite and fused-ring aromatics in tobacco. content; the patented technology "a microbial agent for tobacco and its preparation method and application" discloses a microbial agent prepared from Candida utilis and Bacillus subtilis, which has a certain ability to degrade fused-ring aromatic hydrocarbons ability, but the ability to degrade nitrite is poor, and the ability to improve aroma and tobacco quality is poor, so it is not suitable for promotion and use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The microbial preparation used to improve the quality of tobacco consists of Lactobacillus plantarum fermentation broth and Trichoderma viride fermentation broth mixed uniformly at a volume ratio of 3:2, then 1wt% cellulose is added, cultured at 30°C for 12h, and then added 3 times the volume The Bacillus pumilus fermented liquid, mix evenly, and get it.

[0035] The plantarum lactobacillus fermentation broth is prepared according to the following steps:

[0036] Lactobacillus plantarum seed solution (1 × 10 8 cfu / ml) according to the inoculation amount of 8% volume ratio, transferred to the fermenter for 36 hours to obtain the Lactobacillus plantarum fermentation broth; the fermenter culture medium components are: 5wt% glucose, 2wt% peptone, 1wt% ammonium chloride , potassium dihydrogen phosphate 0.2wt%, 0.05wt% sodium acetate, 0.05wt% magnesium sulfate, 0.01wt% manganese sulfate, the balance is water, and the pH is 6.2;

[0037] The Bacillus pumilus fermented liquid...

Embodiment 2

[0044] A microbial preparation for improving the quality of tobacco, which consists of Lactobacillus plantarum fermentation broth and Trichoderma viride fermentation broth mixed uniformly at a volume ratio of 5:3, then adding 2wt% cellulose, cultivating at 30°C for 12 hours, and then adding 2 times the volume The Bacillus pumilus fermented liquid, mix evenly, and get it.

[0045] The plantarum lactobacillus fermentation broth is prepared according to the following steps:

[0046] Lactobacillus plantarum seed solution (1 × 10 8cfu / ml) according to the inoculation amount of 8% volume ratio, transferred to the fermenter for 36 hours to obtain the Lactobacillus plantarum fermentation broth; the fermenter culture medium components are: 5wt% glucose, 2wt% peptone, 1wt% ammonium chloride , potassium dihydrogen phosphate 0.2wt%, 0.05wt% sodium acetate, 0.05wt% magnesium sulfate, 0.01wt% manganese sulfate, the balance is water, and the pH is 6.2;

[0047] The Bacillus pumilus ferment...

Embodiment 3

[0054] The impact of microbial preparation of the present invention on harmful substance content in tobacco:

[0055] Take Example 1 as an example; blank control group: no microbial preparation treatment; comparative example 1 group: only add Lactobacillus plantarum and Trichoderma viride, do not add Bacillus pumilus; comparative example 2: only add Lactobacillus plantarum and Bacillus pumilus Bacillus, without adding Trichoderma viride; Comparative example 3: only adding Trichoderma viride and Bacillus pumilus, without adding Lactobacillus plantarum; Comparative example 4: only adding Trichoderma viride. The method of treating tobacco with the microbial preparation of each comparative example is the same as that of Example 1, and the tobacco samples are the same, which are comparable.

[0056] 1. The degradation rates of cellulose, pectin and protein are shown in Table 1, and the degradation rate=(the content of components contained in the blank control sample-the content of ...

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PUM

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Abstract

The invention belongs to the technical field of microorganisms, and discloses a microbial preparation for improving tobacco quality. The microbial preparation is prepared according to the following process: mixing lactobacillus plantarum fermentation liquor with trichoderma viride fermentation liquor uniformly, then adding cellulose, culturing for a period of time at 25-30 DEG C, then adding bacillus pumilus fermentation liquor, and mixing uniformly to obtain the microbial preparation. The microbial preparation disclosed by the invention not only can improve the tobacco quality and increase fragrance but also can reduce the content of harmful substance in tobacco, thereby serving two purposes.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to microbial preparations for improving the quality of tobacco. Background technique [0002] Nicotiana is a tubular flower family, Solanaceae annual or limited perennial herb, slightly lignified base. Inflorescences terminal, paniculate, many-flowered; capsules ovate or oblong, about as long as persistent calyx. It blooms and bears fruit in summer and autumn. Mainly distributed in South America, South Asia and China. It is an annual or limited perennial herb, all covered with glandular hairs; roots are strong. The role of tobacco is cigarettes. [0003] As we all know, long-term smoking seriously threatens life and health. Smoking can induce lung cancer and laryngitis, bronchitis, emphysema and other respiratory diseases. Various conditions caused by smoking have become a global concern. How to improve the quality and aroma of tobacco while reducing the content of har...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A24B15/20C12R1/25C12R1/885C12R1/07
CPCA24B15/20C12N1/14C12N1/20
Inventor 鲍增明
Owner 湖北京晟生物科技股份有限公司
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