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Application of triterpenoid saponins sourced from schima superba

A technology of triterpene saponins and compounds, applied in the direction of medical preparations containing active ingredients, cosmetics, cosmetic preparations, etc., can solve the problem of no antibacterial biological activity of Schima superba extract, and achieve good antibacterial effect

Inactive Publication Date: 2017-11-28
XIAMEN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no relevant report on the antibacterial biological activity of Schima superba extract

Method used

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  • Application of triterpenoid saponins sourced from schima superba
  • Application of triterpenoid saponins sourced from schima superba
  • Application of triterpenoid saponins sourced from schima superba

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Dry coarse powder 4.5kg of Schima superba stem, heat and reflux 3 times with 95% ethanol (8 times the amount of medicinal material), each time for 2 hours, combine the extracts for 3 times, and concentrate under reduced pressure to obtain 200g of total extract.

[0022] The total extract was dissolved and dispersed with 50% methanol water, extracted with petroleum ether, chloroform, ethyl acetate and n-butanol in sequence to obtain 50 g of petroleum ether layer, 35 g of chloroform layer, 22 g of ethyl acetate layer and 20 g of n-butanol And the water layer 45g. The ethyl acetate fraction was subjected to silica gel column chromatography in a chloroform-methanol system (100:0; 90:10; 80:20; 70:30; 60:40; 50:50; 30:70; 0:100) to obtain Fr.1 -20 fractions. Wherein fraction Fr.12 (3.3g) is subjected to ODS reverse column chromatography (180g, 3.5cm x 40cm) water-methanol system (80:20; 70:30; 60:40; 40:60; 20:80; 0 :100) to obtain fraction Fr.12-1~13. After preparative o...

Embodiment 2

[0027] 1. Structural identification of the compound sasanquasaponin III (SS-9)

[0028]

[0029] White amorphous powder, (c 0.30, MeOH). Both Liebermann-Burchard and Molish reactions were positive, and the color of concentrated sulfuric acid-vanillin was red (TLC), which was speculated to be triterpenoid saponins. ESI-MS m / z1241.6[M+Na] + , suggesting that the molecular weight may be 1218, combined with 1 H NMR and 13 C NMR spectrum speculates that the molecular formula of compound SS-9 is C 59 h 94 o 26 .

[0030] 1 H NMR spectrum shows 7 angular methyl hydrogen signals [δ H 0.81(3H,s,H-25),1.03(3H,s,H-26),1.08(3H,s,H-29),1.08(3H,s,H-24),1.30(3H,s, H-23), 1.43(3H,s,H-30), 1.85(3H,s,H-27)], 1 ene hydrogen signal (δ H 5.48,br.s), four terminal hydrogen signals [δ H 4.92 (d, J=7.1Hz, H-1′), 5.95 (d, J=7.6Hz, H-1″), 6.19 (d, J=7.0Hz, H-1″′) and 6.26 (br. s,H-1″’)], a set of characteristic angelica acyl hydrogen signals [δ H 1.88(s, H 3 -5″″′), 2.05(d, J=6.6H...

Embodiment 3

[0056] Antifungal Activity Test of Monomer Compounds in Vitro

[0057] In this experiment, sensitive strains of Candida albicans and resistant strains of Candida albicans were used as indicator bacteria, and the in vitro drug susceptibility test of the disk method was used to determine the active monomer compounds, and then the MIC value and MFC of the active monomer compounds were determined by direct visual turbidimetry. value.

[0058] 1. Instruments and experimental materials

[0059] 1.1 Main instruments and equipment

[0060] AIRTECH ultra-clean workbench (Suzhou Su Cleaning Equipment Co., Ltd.); BS200 electronic analytical balance (Sartorius, Germany); LDZX-40SC vertical self-control electric pressure steam sterilizer (Shanghai Shen'an Medical Instrument Factory); induction cooker (Zhongshan Nuojieshi Electric Co., Ltd.); 96-well cell culture plate (Coning Costar, USA); precision pipette (Gilson, France); BDS200 inverted biological microscope (Chongqing Aote Optical I...

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Abstract

The invention discloses an application of triterpenoid saponins sourced from schima superba. The triterpenoid saponins are extracted from schima superba and preferably from roots and / or stems of the schima superba. Preferably, ethyl alcohol is 95% ethyl alcohol. The triterpenoid saponins have good antibacterial function.

Description

technical field [0001] The invention relates to the field of medicinal materials, in particular to the application of a triterpene saponin compound derived from Schima superba. Background technique [0002] Fungal infection, especially deep fungal infection and fungal drug resistance, is a major problem facing human health and one of the main causes of human disease and death. In recent years, with the widespread use of immunosuppressants, the widespread development of tumor radiotherapy and chemotherapy, especially the increasing number of AIDS patients, the incidence of fungal infections, especially deep fungal infections, has increased sharply [1] . Statistics show that in a tertiary hospital in my country, from 2012 to 2014, the mortality rate due to fungal infection in the hospital reached 12.88%. [2] . Fungal infection has become one of the main causes of death in patients with the above-mentioned immunocompromised critical diseases [1-3] . While antifungal drugs ...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P31/10A61K8/60A61Q17/00
CPCA61K8/602A61K31/704A61Q17/005
Inventor 吴春李黎明王雅英
Owner XIAMEN MEDICAL COLLEGE
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