Ellagic acid prepared through liquid-state fermentation of granatanine and preparation method thereof
A technology of liquid fermentation and ellagic acid, which is applied in the fields of cosmetics, health care products, and pharmaceuticals. It can solve the problems of high-concentration strong acid consumption, uneven fermentation, troublesome loading and discharging, etc., and achieves increased utilization times, mild fermentation conditions, The effect of improving utilization efficiency
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Embodiment 1
[0023] (1) Preparation of pomegranate peel tannin extract
[0024] Grind the dried pomegranate peel to 40 mesh, use 62% ethanol solution at 72°C to reflux extract three times, add ethanol solution for the first time which is 12 times the mass of pomegranate peel powder, extract for 2 hours, and centrifuge to separate the filtrate and filter residue; For the second time, add 10 times of ethanol solution to the filter residue, extract for 1.5 h, and centrifuge; for the third time, add 8 times of ethanol solution to the filter residue, extract for 1 h, and centrifuge; combine the filtrates from three times, recover ethanol by distillation, and obtain pomegranate The concentrated aqueous solution of leather tannin extraction can be directly used in fermentation production. The tannin content in the extracted concentrated aqueous solution is determined to be 1% to 1.5%.
[0025] (2) Cultivation and co-immobilization of fermentation strains
[0026] After Aspergillus niger, Penici...
Embodiment 2
[0032] (1) Preparation of pomegranate peel tannin extract
[0033] Grind the dried pomegranate peel to 40 mesh, use: 65% ethanol solution at 73°C for reflux extraction three times, add ethanol solution for the first time which is 14 times the mass of pomegranate peel powder, extract for 2 h, and centrifuge the filtrate and filter residue Add 9 times of ethanol solution to the filter residue for the second time, extract for 1.5 h, and centrifuge; add 7 times of ethanol solution to the filter residue for the third time, extract for 1 h, and centrifuge; combine the filtrates for three times, recover ethanol through distillation, and obtain The concentrated aqueous solution of pomegranate peel tannin extraction can be directly used in fermentation production. The tannin content in the extracted concentrated aqueous solution is determined to be 1% to 1.5%.
[0034] (2) Cultivation and co-immobilization of fermentation strains
[0035] Aspergillus niger, Penicillium and Escherichi...
Embodiment 3
[0041] (1) Preparation of pomegranate peel tannin extract
[0042] Grind the dried pomegranate peel to 40 mesh, use 70% volume fraction of ethanol solution to reflux extract at 75 ℃ for three times, add ethanol solution for the first time which is 15 times the mass of pomegranate peel powder, extract for 2 h, and centrifuge to separate the filtrate and filter residue; Add 8 times of ethanol solution to the filter residue for the second time, extract for 1.5 h, and centrifuge; add 6 times of ethanol solution to the filter residue for the third time, extract for 1 h, and centrifuge; combine the filtrates from three times, recover ethanol by distillation, and obtain pomegranate The concentrated aqueous solution of leather tannin extraction can be directly used in fermentation production. The tannin content in the extracted concentrated aqueous solution is determined to be 1% to 1.5%.
[0043] (2) Cultivation and co-immobilization of fermentation strains
[0044] After Aspergill...
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