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Antibacterial giant salamander secretion biofilm and its preparation method and application

A technology of secretions and biofilms, applied in applications, pharmaceutical formulations, drug delivery, etc., can solve problems such as difficult to uniformly disperse antibacterial agents

Active Publication Date: 2020-09-01
武汉华联科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the insoluble properties of giant salamander secretions, researchers continue to try to prepare giant salamander secretions into films, but it is difficult to form giant salamander secretions without destroying the original properties of giant salamander secretions such as adhesion and biocompatibility. biofilm
In addition, due to the insolubility of giant salamander secretions, it is difficult to uniformly disperse antibacterial agents in giant salamander secretions, and it is difficult to form antibacterial agents with good antibacterial effects without destroying the original properties of giant salamander secretions such as adhesion and biocompatibility. giant salamander secretion

Method used

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  • Antibacterial giant salamander secretion biofilm and its preparation method and application
  • Antibacterial giant salamander secretion biofilm and its preparation method and application
  • Antibacterial giant salamander secretion biofilm and its preparation method and application

Examples

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preparation example Construction

[0034] see figure 1 According to one embodiment, the preparation method of antibacterial giant salamander secretion biofilm includes the following steps S110-S130.

[0035] S110, dissolving the giant salamander secretion in an acidic solution or a fluorine-containing polar solvent to obtain a giant salamander secretion solution, wherein the acidic solution is selected from vitamin C solution, hydrochloric acid solution, acetic acid solution, sulfuric acid solution, phosphoric acid solution and nitric acid solution At least one, the fluorine-containing polar solvent is a fluorine-containing organic solvent containing carboxyl or hydroxyl groups;

[0036] In one embodiment, the giant salamander secretion is dry powder of giant salamander mucus, and the concentration of the giant salamander secretion in the giant salamander secretion solution is 0.001 g / mL˜2 g / mL.

[0037] In one embodiment, the concentration of the giant salamander secretion in the giant salamander secretion so...

Embodiment 1

[0117] Clean the body surface of the fresh giant salamander with clean water and remove impurities such as molting, and then scrape the body surface of the giant salamander back and forth with a spoon. When the body surface of the giant salamander secretes a large amount of milky white mucus, scrape and collect the giant salamander mucus. Generally, about 5g of giant salamander mucus can be collected from about 5kg of adult giant salamander each time, and a total of 40g of giant salamander mucus can be obtained by scraping. The collected giant salamander mucus was frozen in a -20°C refrigerator for 0.5 h. The frozen giant salamander mucus was placed in a freeze dryer, and vacuum freeze-dried for 36 hours at a temperature of -40° C. and an absolute pressure of 10 Pa to obtain dry powder of the giant salamander mucus. The dry powder of giant salamander mucus was pulverized with a low-temperature ultrafine pulverizer, and liquid nitrogen was added while pulverizing to keep the te...

Embodiment 2

[0122] Giant salamander secretions were prepared according to the method in Example 1.

[0123] Take 0.5g of giant salamander secretion and add it to 20mL of hydrochloric acid solution, wherein the concentration of hydrogen chloride in the hydrochloric acid solution is 1mol / L. Stir at room temperature (20°C) for 4 hours to form a homogeneous giant salamander secretion solution.

[0124] Take 5mL giant salamander secretion solution and pour it into a molding mold with a length×width of 1cm×1cm, then put the molding mold into a freeze dryer, cool it down to -30°C at a rate of 0.5°C / min, and put it under the temperature condition of -30°C Place in the freezer for 0.5h. Subsequently, the forming mold was placed under the condition of an absolute pressure of 10 Pa for 12 hours to obtain a membrane-like giant salamander secretion biofilm with uniform texture and micropores.

[0125] At room temperature (20° C.), the giant salamander secretion biofilm was immersed in a solution of ...

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Abstract

The invention discloses an antibacterial andrias davidianus secretion biological membrane and a preparation method and the application thereof. The method comprises the following steps: dissolving andrias davidianus secretion into an acidic solution or a fluorine-containing polar solvent to obtain an andrias davidianus secretion solution; performing freeze-drying on the andrias davidianus secretion solution to obtain a membrane, so as to obtain the andrias davidianus secretion biological membrane; then wetting the andrias davidianus secretion biological membrane through an antibacterial agent so as to obtain the antibacterial andrias davidianus secretion biological membrane. The preparation method of the antibacterial andrias davidianus secretion biological membrane is simple and convenient; an original structure of the andrias davidianus secretion is not damaged; the prepared antibacterial andrias davidianus secretion biological membrane remains the advantages of high adhesiveness and biocompatibility of the andrias davidianus secretion; the antibacterial agent can be uniformly dispersed and absorbed in the andrias davidianus secretion biological membrane; the andrias davidianus secretion biological membrane is high in toughness and intensity and can be used as a haemostatic membrane, a tissue adhering membrane or a bandage and the like for covering or wrapping a wound, so that the membrane has a good application prospect.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to an antibacterial giant salamander secretion biofilm and its preparation method and application. Background technique [0002] The giant salamander, also known as the "Chinese giant salamander," belongs to the giant salamander family and is one of the largest living amphibian species. It shows great research progress and valuable potential in the fields of biodiversity, genetic evolution and biochemistry. Giant salamander mucus is a kind of giant salamander secretion after the body surface of the giant salamander is stimulated. Previous studies have found that the giant salamander secretion has the advantages of good adhesion, good biocompatibility, and degradability. It can be developed into hemostatic materials, adhesives and Soft tissue filling materials, etc. [0003] However, the traditional application forms of giant salamander secretions are mostly powder or gel. Clin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/40A61L15/42A61L24/00
CPCA61L15/40A61L15/42A61L24/0005A61L24/001A61L2300/104A61L2300/404A61L2300/412A61L2300/418A61L2400/04
Inventor 邢孟秋商海涛蒋坤魏泓
Owner 武汉华联科生物技术有限公司
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