Unlock instant, AI-driven research and patent intelligence for your innovation.

Vegetable food ningnanmycin residue measuring method

A Ningnanmycin residue technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of no Ningnanmycin residue detection method

Active Publication Date: 2017-12-12
PONY TESTING INT GRP SHANGHAI CO LTD
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But at present, there is no detection method for Ningnanmycin residues in food

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vegetable food ningnanmycin residue measuring method
  • Vegetable food ningnanmycin residue measuring method
  • Vegetable food ningnanmycin residue measuring method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Instruments and reagents

[0055] High performance liquid chromatography-tandem mass spectrometer: American Waters, UPLC-Xevo TQ;

[0056] Standard substance: Ningnanmycin, purity 98.5%;

[0057] Methanol, ammonium acetate: chromatographically pure;

[0058] Acetic acid is analytically pure;

[0059] Anhydrous magnesium sulfate is analytically pure;

[0060] PSA is analytically pure;

[0061] HLB solid phase extraction cartridge: 6mL, 200mg;

[0062] The water used in this method is primary water.

[0063] 2. Instrument analysis conditions

[0064] Liquid chromatography conditions:

[0065] Chromatographic column: Waters, BEH C18, 1.7μm, 2.1mm×50mm

[0066] Mobile phase: 5mmol / L ammonium acetate aqueous solution, acetonitrile

[0067] Flow rate: 0.4mL / min;

[0068] Injection volume: 5.0μL;

[0069] Column temperature: 40°C;

[0070] See Table 1 for gradient elution

[0071] Table 3 Gradient elution

[0072]

A: 5mmol / L ammonium acetate aqueous...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of food safety and relates to a vegetable food ningnanmycin residue measuring method. The vegetable food ningnanmycin residue measuring method comprises weighing out a uniform sample, performing homogenization extraction through acidic methanol solution at a pH of 4.5, absorbing interfering substance through anhydrous magnesium sulfate and PSA (N-propylethylene diamine), performing high-speed refrigerated centrifugation, rotatably evaporating supernatant liquid to an almost dry state, dissolving residues in water, performing purification through HLB (hydrophilic-lipophilic balance) purification columns, performing elution and liquid nitrogen blowing to a dried state, performing liquid-phase dissolved volume fixing, and then performing a filtering process through a 0.22 mu m organic filter membrane to obtain a material to be detected; detecting the material to be detected through a liquid chromatograph / mass spectrometer and performing quantitation through an external reference method. The vegetable food ningnanmycin residue measuring method is simple and novel in pretreatment processes, good in impurity removal effects, high in sensitivity and recovery rate, good in precision of measured results and capable of effectively detecting the ningnanmycin residue of various fruits, vegetables and crops.

Description

technical field [0001] The invention belongs to the technical field of food safety, and in particular relates to a method for determining Ningnanmycin residues in fruits, vegetables and grains by liquid chromatography tandem mass spectrometry. technical background [0002] Ningnanmycin is a new cytosine nucleoside peptide antibiotic. It is a white powder with a melting point of 195°C. It is easily soluble in water, soluble in methanol, slightly soluble in ethanol, and insoluble in acetone, ethyl ester, benzene and other organic compounds. Solvent, pH3.0-5.0 is relatively stable, easy to decompose and lose activity when alkaline. [0003] Ningnanmycin is a patented technology product successfully developed by the Chengdu Institute of Biology, Chinese Academy of Sciences, after the Seventh Five-Year, Eighth Five-Year, and Ninth Five-Year National Science and Technology Research. It is currently used in tobacco mosaic virus disease, tomato virus disease, pepper virus disease, r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06
CPCG01N30/06
Inventor 宋薇邹强王超李庆
Owner PONY TESTING INT GRP SHANGHAI CO LTD