Novel method for producing balsampear polypeptide protein extract at low temperature in whole course, balsampear polypeptide protein extract and application thereof

Abstract

The invention provides a novel method for producing balsampear polypeptide protein extract at a low temperature in the whole course, the balsampear polypeptide protein extract and application thereof, and relates to the technical field of food deep processing. The method comprises the following steps: washing a balsampear raw material, pulping and grinding, putting into a temperature-controllable ultrasonic-assistant extraction tank, adding a neutral buffer solution for extracting, and centrifugally removing slag to obtain centrifugate; filtering the centrifugate through a microfiltration membrane system to obtain microfiltration membrane permeate; filtering the permeate through two roll-to-roll ultrafiltration membrane purification systems having different molecular weights to obtain ultrafiltration membrane trapped fluid; concentrating the trapped fluid with a roll-to-roll high-pressure reverse osmosis permeable membrane to obtain a concentrate having a solid content of more than or equal to 30 percent; and drying the concentrate. The method is operated at normal temperature, and natural activity and more than 20 percent of content of the balsampear polypeptide protein can be effectively guaranteed; the produced balsampear extract has the function of regulating blood glucose, and can be applied to special dietary foods, health foods or medicines for treating or preventing diabetes mellitus.

Description

technical field [0001] The invention relates to the technical field of deep food processing, and in particular to a new method for producing a bitter melon polypeptide protein extract at low temperature throughout the whole process, the bitter melon polypeptide protein extract and applications thereof. Background technique [0002] Bitter gourd, also known as bitter gourd, belongs to Cucurbitaceae. It is an annual climbing plant. The fruit is oblong and yellow when ripe. There are many irregular tumor-like protrusions on the surface, and the seeds are hidden in the fleshy fruit. Bitter gourd is bitter in taste and cold in nature. It is a vegetable with both medicinal and edible uses. According to "Compendium of Materia Medica" and "Southern Yunnan Materia Medica", bitter gourd has the functions of clearing away heat and relieving heat, nourishing blood and nourishing qi, nourishing kidney and spleen, nourishing liver and improving eyesight; bitter gourd contains trypsin inhi...

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Problems solved by technology

[0004] Although scientific and technical personnel have realized that the bitter melon polypeptide extract extracted from balsam pear can be used to prevent and treat diabetes, and there are many balsam pear extracts and corresponding products on the market, but there are some problems at the same time: first, the above-mentioned literature and other related The preparation method of bitter gourd polypeptide is mostly obtained by various enzymatic hydrolysis reactions, alkali extraction and acid precipitation, or combined with other sedimentation methods. The preparation time period is long or some steps such as enzyme inactivation operation temperature are high, and the bitter gourd pulp is prepared from the bitter gourd seeds. The peptide protein content of the extract is not high, rarely exceeding 10%, and the molecular weight distribution is not clear

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