A kind of chitosan-based composite nerve guide and its preparation method and application

A nerve conduit, chitosan technology, applied in prosthesis, tissue regeneration, medical science and other directions, can solve problems such as unfavorable nerve growth and repair, unfavorable nerve growth, etc. good support effect

Active Publication Date: 2020-10-02
QINGDAO HUISHENG HUIZHONG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently available materials are still unsatisfactory. For example, there may be risks of immunogenicity, and local acidity or local inflammation produced by degradation products is not conducive to nerve growth. It is difficult to provide a good environment for nerve growth and is not conducive to nerve growth and repair. No ideal nerve conduit to facilitate nerve repair enters practical application

Method used

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  • A kind of chitosan-based composite nerve guide and its preparation method and application
  • A kind of chitosan-based composite nerve guide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Preparation of acylated chitosan tube: take by weighing 2 g of acylated chitosan powder (90.5% acetyl group molar percentage), add 100 ml of 80% formic acid solution, stir and dissolve, and prepare a mass volume ratio of 2% acylated chitosan glue. Make a hollow acylated chitosan tube with a wall thickness of 0.5 mm and a diameter of 4 mm on a tube making machine, place the dry acylated chitosan tube in 2% NaOH aqueous solution for acid-base neutralization, wash with water until the pH is neutral, Dry to produce acylated chitosan tubes.

[0023] In the preparation of the acylated chitosan tube, the same effect can be achieved by using the following acylated chitosan, the acylated chitosan can be acetyl chitosan, propionyl chitosan, butyryl shell Polysaccharide, hexanoyl chitosan, octanoyl chitosan, caproyl chitosan, lauroyl chitosan, palmitoyl chitosan and other aliphatic or aromatic acyl chitosan or one or more Mixtures, or acylated shells containing one...

Embodiment 2

[0026] Embodiment 2: Preparation 1 of chitosan-based composite nerve guide

[0027]Weigh 2 g of acetylated chitosan powder (90.5% molar content of acyl groups), add 100 ml of 80% formic acid solution as a solvent, stir and dissolve, and prepare an acylated chitosan glue solution with a concentration of 2% by mass. Make a hollow acylated chitosan tube with a wall thickness of 0.05 mm, a lumen diameter of 2 mm, and a length of 10 cm on a tube making machine, dry it naturally, and place the dried acylated chitosan tube in 2% NaOH aqueous solution for acid-base Neutralize, wash with water until the pH is neutral, and dry to prepare the acylated chitosan tube 1.

[0028] The polysaccharide fiber calcium alginate fiber loaded with calcium is taken, and the calcium alginate fiber is loosely filled in the acylated chitosan tube 1 along the direction, and the chitosan-based composite nerve guide 1 is prepared.

Embodiment 3

[0029] Embodiment 3: Preparation 2 of chitosan-based composite nerve guide

[0030] Weigh 5 g of butyrylated chitosan powder (125.2% molar content of acyl groups), add 100 ml of acetone solution as a solvent, stir and dissolve, and prepare an acylated chitosan glue solution with a concentration of 5% by mass. A hollow acylated chitosan tube with a wall thickness of 0.5 mm, a lumen diameter of 4 mm, and a length of 10 cm was made on a tube making machine, and heated and dried at 50° C. to obtain an acylated chitosan tube 2 .

[0031] The calcium-loaded polysaccharide fiber carboxymethyl cellulose calcium fiber is taken, and the carboxymethyl cellulose calcium fiber is loosely filled in the acylated chitosan tube 2 along the direction to prepare the chitosan-based composite nerve guide 2 .

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Abstract

The invention discloses a chitosan-based composite nerve conduit as well as a preparation method and an application thereof. The chitosan-based composite nerve conduit is formed by compositing an acrylated chitosan conduit wall and internal filler of calcium-supported polysaccharide fiber. Acrylated chitosan is one of or a mixture of more of aliphatic or aromatic acrylated chitosans, and the mole percentage of acryl groups is larger than 0 and smaller than 300%; the calcium-supported polysaccharide fiber is calcium carboxylate, calcium sulfonate or calcium succinate supported polysaccharide fiber. The chitosan-based composite nerve conduit is used as a nerve conduit or a nerve bridging conduit for defective nerve repair. The degradation time of the acrylated chitosan conduit wall is longer than that of the chitosan conduit wall, the support effect of the nerve conduit is enhanced and prolonged, meanwhile, degradation inflammation reactions are slight, and the in-vivo biological tissue compatibility is good; the calcium-supported polysaccharide fiber is beneficial to guide of growth of nerve fiber and a good growth environment is provided.

Description

technical field [0001] The invention relates to a nerve repair medical material, in particular to a chitosan-based composite nerve guide and its preparation method and application, belonging to the field of biomedical materials. Background technique [0002] Peripheral nerve defects caused by mechanical trauma, traffic accidents, etc. are one of the common traumatic manifestations. After nerve injury, the distal limbs dominated by the affected nerves suffer from loss of sensory and motor functions, resulting in physical disabilities and serious quality of life for patients. decline. The defect of a small segment of nerve can be restored by the self-repair ability of the nerve, while the repair of a long segment of defective nerve cannot be repaired by itself. It needs to be modified by surgical autologous nerve transplantation or implantation of nerve bridges to guide the growth of the proximal regenerated nerve. . Nerve conduits are a hotspot in the research of nerve brid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/54A61L27/58
CPCA61L27/20A61L27/54A61L27/58A61L2300/102A61L2300/236A61L2300/41A61L2300/412A61L2300/604A61L2430/32C08L5/08
Inventor 刘万顺
Owner QINGDAO HUISHENG HUIZHONG BIOLOGICAL TECH CO LTD
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