Evaluation Method of Bioavailability of Fukeqianjin Tablets Combined with Antibiotics

Abstract

The invention discloses a method for evaluating the bioavailability in combined application of fuke qianjin tablets and antibiotics. According to the method, the concentration of a drug to be detected in a blood plasma sample is detected, the pharmacokinetic parameters are calculated by using an ADAPT 5.1 software inverse Gaussian model, and each group of the parameters are subjected to statistical analysis with SPSS software so as to complete the evaluation, wherein the blood plasma sample is obtained after the single administration of antibiotic drugs or the combined administration of fuke qianjin tablets and antibiotics, the concentration of the drug to be detected is the concentration of the antibiotic drug, and the method for detecting the concentration of the drug to be detected in the blood plasma sample is a HPLC-ECD method or HPLC-ESI-MS method. According to the present invention, the pharmacokinetic evaluation method for the multiple administration of fuke qianjin tablets and azithromycin in chronic pelvic inflammatory disease rats is established, and the reliable method for evaluating the rationality of the combined administration of fuke qianjin tablets and azithromycin is achieved; and with the evaluation method, the pharmacokinetic rule of fuke qianjin tablets and azithromycin in chronic pelvic inflammatory disease rats can be successfully obtained, the scientific basis can be provided for the reasonable clinical application of fuke qianjin tablets and azithromycin, and the reference can be provided for the subsequent related research.

Description

technical field [0001] The present invention relates to the technical field of pharmacokinetic evaluation, in particular to an evaluation method of bioavailability in the combined application of traditional Chinese medicine prescriptions and antibiotics, and more specifically, to an evaluation method of bioavailability in the combined application of Fuke Qianjin Tablets and antibiotics. Background technique [0002] Chronic pelvic inflammatory disease (CPID) is a common disease in gynecology. The main clinical manifestations are lower abdominal pain and lumbosacral pain. Its pathogenesis is relatively complicated. Modern medical research believes that the disease is mainly caused by pathogenic bacteria. Antibiotics are the main treatment. Traditional Chinese medicine believes that damp-heat stagnation and qi stagnation and blood stasis are the main causes of the disease. Fuke Qianjin Tablets are mainly composed of eight herbs such as Qianjinba, Andrographis paniculata, Rosa ...

AI Technical Summary

Problems solved by technology

[0003] At present, studies on the combined application of Fuke Qianjin Tablets and antibiotics mostly focus on the influence of the combination of the two on pharmacodynamics, while there are few reports on the influence of pharmacokinetics

The in vivo process of a drug has been shown to be significantly affected by gender, age, disease state, and drug combination, thereby affecting its bioavailability

In previous studies on the effect of drug combination on pharmacokinetics, a single dose of healthy animals was often used. This method has more sampling points and has a greater impact on the physiological state of the tested animals. The reliability and practicability of the evaluation cannot be guaranteed.

[0004] As a compound preparation, Fukeqianjin Tablets have a lot of medicinal flavors and complex ingredients. After a long period of compatible use with antibiotics (azithromycin), they may interact with each other, resulting in changes in the pharmacokinetic process. However, the existing single-dose evaluation method does not It cannot fully simulate and reflect the actual situation of long-term multiple administration in the clinical disease state, and the obtained results have little guiding significance for clinical medication

[0005] At present, there is a lack of a simple, feasible, long-term multiple-dose evaluation method that conforms to the clinical dosing regimen, so that it is possible to quantitatively study the effects of Fukeqianjin Tablets and azithromycin on the pharmacokinetics and bioavailability of the main antibacterial active ingredient azithromycin There is a lack of powerful reference research results and literature on the rationality of the clinical compatibility of the two, and the pharmacokinetic research model of the combination of Chinese and Western medicines in this field.

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